2016
DOI: 10.1021/acsbiomaterials.6b00226
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Direct Production of Human Cardiac Tissues by Pluripotent Stem Cell Encapsulation in Gelatin Methacryloyl

Abstract: Direct stem cell encapsulation and cardiac differentiation within supporting biomaterial scaffolds are critical for reproducible and scalable production of the functional human tissues needed in regenerative medicine and drug-testing applications. Producing cardiac tissues directly from pluripotent stem cells rather than assembling tissues using pre-differentiated cells can eliminate multiple cell-handling steps that otherwise limit the potential for process automation and production scale-up. Here we asked wh… Show more

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Cited by 47 publications
(27 citation statements)
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“…3D scaffold of GelMA hydrogels can guide the formation of a desired tissue due to their attachment sites and signaling cues. A variety of applications of GelMA hydrogels had been reported in tissue engineering, such as bones [44,45,46,47,48,49,50,51,52,53,54,55,56,57], endochondral bone [58,59], skin [60,61,62,63,64,65], myocardium [66], cardiac tissues [67,68,69,70,71,72], cartilage [42,73,74,75,76,77,78,79,80,81], vascular networks [82,83,84,85,86,87,88,89], skeletal muscle [90,91,92,93], cornea [94,95], interface [96] and so on. Ovsianikov A et al [44] prepared 3D CAD scaffolds for tissue engineering applications using two-photon polymerization (2PP).…”
Section: Applications Of Gelma Hydrogelsmentioning
confidence: 99%
“…3D scaffold of GelMA hydrogels can guide the formation of a desired tissue due to their attachment sites and signaling cues. A variety of applications of GelMA hydrogels had been reported in tissue engineering, such as bones [44,45,46,47,48,49,50,51,52,53,54,55,56,57], endochondral bone [58,59], skin [60,61,62,63,64,65], myocardium [66], cardiac tissues [67,68,69,70,71,72], cartilage [42,73,74,75,76,77,78,79,80,81], vascular networks [82,83,84,85,86,87,88,89], skeletal muscle [90,91,92,93], cornea [94,95], interface [96] and so on. Ovsianikov A et al [44] prepared 3D CAD scaffolds for tissue engineering applications using two-photon polymerization (2PP).…”
Section: Applications Of Gelma Hydrogelsmentioning
confidence: 99%
“…Using the established microfluidic encapsulation platform, microspheres have been produced using multiple photocrosslinkable polymers that have been widely employed for various tissue engineering applications . Here, we examined the photocrosslinkable hydrogel materials PF, GelMA, and PEGDA; these materials have been used for cancer tissue engineering, cardiac tissue engineering, and bone regeneration . These polymers were selected to demonstrate the compatibility of this setup with various polymers.…”
Section: Resultsmentioning
confidence: 99%
“…For instance, using fluorescent bead density as a readout for stiffness gradients in gels instead of using expensive techniques like AFM (Barber-Pérez et al, 2020). Recent technology also allows assessment of mechanical properties of materials with extremely precise force (resolution down to 10 nN claimed by the manufacturer) through micro-scale compression testing, particularly for soft biomaterials (Kerscher et al, 2017).…”
Section: Conclusion and Outlooksmentioning
confidence: 99%