Direct radioimmunoassay of estradiol in serum extracts

Zucchelli, G.C.; Piro, M.A.; Simonini, N.; Giannessi, Daniela; Malvano, R.

doi:10.1007/bf00257280

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…In fact, the precision level observed for unconjugated and total E4 agrees with that of other RIA measurements routinely carried out at our laboratory for steroid determinations (8,9,12). Moreover, the accuracy of these methods has been widely proved by recovery and serial dilution tests, by evaluation of steroidal interferences and by evaluation of blank effects (serum, solvent, ANS); where necessary, the blank effects have been acceptably cor rected by using a compensated standard.…”

Section: Discussionsupporting

confidence: 84%

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Radioimmunoassay of Unconjugated and Total Serum Estetrol Using a <sup>125</sup>I-Iodinated Tracer

Zucchelli¹,

Giannessi²,

Piro³

et al. 1979

Horm Res

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A radioimmunoassay (RIA) for the measurement of both unconjugated and total serum estetrol has been developed, using an antiserum to an E₄-3-conjugate and a ¹²⁵I-radioiodinated E₄ tracer. Assay of dried ethyl ether extracts was used for the determination of unconjugated E₄, while a direct measurement of unextracted hydrolyzed serum in the presence of 0.3% 8-anilino-1-naphthalene sulphonic acid (ANS) proved adequate for total E₄. Assay reliability was evaluated and the procedure standardized through a series of tests aimed at assessing accuracy, sensitivity and precision. No steroidal interference was found to practically affect the assay (0.3% estriol cross-reactivity), nor were solvent and sample blanks observed in the case of unconjugated E₄. For total E₄ assay, the sample blank effects were acceptably overcome by using hydrolyzed male serum and 0.3% ANS, as a standard diluent. An interassay variability amounting to approximately 10 and 6% resulted for unconjugated E₄ and total E₄ RIA, respectively. A number of serum samples (285 for unconjugated E₄, 147 for total E₄) randomly collected throughout normal pregnancy were assayed. The unconjugated E₄ levels at 15 th week and at term were 62.7 ± 22.6 and 766.5 ± 208.2 (SD) pg/ml, respectively. Total E₄ was about 6-7 times higher than the levels of free E₄ and increased 7 times from the 15th week to term.

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Section: Discussionsupporting

confidence: 84%

“…The procedure was the same as that already employed in our laboratory for the assay of other steroidal hormones (8,9). 0.20-ml aliquots of serum samples were extracted with 2 ml of diethyl ether in polyethylene-capped glass tubes by rotatory agitation for 30 min.…”

Section: Assay O F Unconjugated /:'4mentioning

confidence: 99%

Radioimmunoassay of Unconjugated and Total Serum Estetrol Using a <sup>125</sup>I-Iodinated Tracer

Zucchelli¹,

Giannessi²,

Piro³

et al. 1979

Horm Res

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Differences in Insulin Receptors between Men and Menstruating Women and Influence of Sex Hormones on Insulin Binding during the Menstrual Cycle*

Bertoli

Fusco

Greco

et al. 1980

The Journal of Clinical Endocrinology & Metabolism

114

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Specific binding of [125I]insulin to circulating monocytes and erythrocytes from nine normal menstruating women and nine normal men was determined during a 28-day period (one sample every 7 days). In women, insulin binding was higher to both monocytes (P less than 0.001) and erythrocytes (P less than 0.02) in the follicular phase than in the luteal phase. In men, insulin binding to monocytes was similar to the follicular phase values for women; however, insulin binding to erythrocytes from men showed higher values than insulin binding to erythrocytes from women in both the follicular (P less than 0.001) and luteal (P less than 0.001) phases. These differences were due primarily to changes in receptor concentration rather than receptor affinity. An inverse relationship was found between insulin binding to monocytes and levels of 17 beta-estradiol, progesterone, and 17 alpha-hydroxyprogesterone; this relationship was not observed in insulin binding to erythrocytes. The present data, therefore, suggest that sex hormones may play a role in the control of insulin receptors. Furthermore, it appears that other factors exist during the follicular phase that lower insulin binding to erythrocyte insulin receptors. If insulin receptors on circulating cells reflect the behavior of the main insulin target tissues, the present data might in part explain the reduction in glucose tolerance reported by various authors in the second half of the menstrual cycle.

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Study on Clinical Features and the Reproductive Hormonal Profile in Normal, Overweight and Obese Polycystic Ovarian Syndrome Women of Selected Age Group

Velvizhi

Annapurani

2021

IJND

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The objectives of the present study are to compare the clinical features among normal and PCOS women and to assess the reproductive hormonal profile of normal and PCOS women of the overweight and obese group. The normal and PCOS women were subdivided into three groups, group 1-healthy women with BMI 18.5- 24.99 (n=10), group 2 PCOS with BMI 25-29.9 (n=11) and group 3 PCOS with BMI >29.9 (n=10). The prevalence of menstrual irregularity, acne, hirsutism and obesity are the major clinical manifestations of PCOS women than the normal women of the same age group. The levels of testosterone increased and estradiol level decreased in the PCOS subjects of both overweight and obese groups. These observations in the study strongly support the presence of hyperandrogenism in PCOS women and favors all the associated clinical features. The results also clearly demonstrated the increase in body mass index reflecting a negative impact on the reproductive outcome in PCOS.

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Direct radioimmunoassay of estradiol in serum extracts

Cited by 3 publications

References 15 publications

Radioimmunoassay of Unconjugated and Total Serum Estetrol Using a <sup>125</sup>I-Iodinated Tracer

Radioimmunoassay of Unconjugated and Total Serum Estetrol Using a <sup>125</sup>I-Iodinated Tracer

Differences in Insulin Receptors between Men and Menstruating Women and Influence of Sex Hormones on Insulin Binding during the Menstrual Cycle*

Study on Clinical Features and the Reproductive Hormonal Profile in Normal, Overweight and Obese Polycystic Ovarian Syndrome Women of Selected Age Group

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