Direct sampling mass spectrometry for clinical analysis

Abstract

Direct sampling mass spectrometry enables high-performance clinical analysis, such as imaging, drug monitoring and point-of-care testing.

Single Cell Metabolomics: A Future Tool to Unmask Cellular Heterogeneity and Virus-Host Interaction in Context of Emerging Viral Diseases

“…Direct sampling through nano-electrospray ionization tips followed by mass spectrometric analyses method is gaining popularity in recent times due to its minimum sample requirement and processing along with high throughput data generation. Pipette tip column electrospray ionization (PTC-ESI) or ballpoint electrospray ionization (BP-ESI) followed by simultaneous mass spectrometry or live single-cell video-mass spectrometry are the SCM platforms where the technique has been used (Tsuyama et al, 2008;Huang et al, 2012;Ji et al, 2016;Pu et al, 2018).…”

One-step hexaplex immunoassays by on-line paper substrate-based electrospray ionization mass spectrometry for combined cancer biomarker screening

“… 20,21 The emergence of ambient ionization makes a simple, flexible and sample-oriented setup possible for MS detection. 22,23 Among these techniques, paper spray ionization (PSI) is recognized as one of the simplest ionization forms 24–27 and has been demonstrated to be suitable for multiplexed immunoassays carried out on paper, 28–30 but challenges remain for clinical detection. One big challenge is the paper-to-paper reproducibility because the paper tip shapes and the reactive areas are critical for accurate quantitative detection.…”

Solvent‐resistant microfluidic paper‐based analytical device/spray mass spectrometry for quantitative analysis of C18‐ceramide biomarker

“…A huge amount of attention has been devoted to the miniaturization of portable MS instrumentation and to the development of direct sampling ionization. 22 Soft ionization methods such as matrix-assisted laser desorption/ionization MS 23 and electrospray ionization MS 24 have been widely exploited for the analysis of biological samples 25,26 because of their high sensitivity and relatively fast operation time. Recently, desorption electrospray ionization and electrospray-assisted laser desorption/ionization methods have been reported for the analysis of biological samples.…”

“…MS is a dominant instrument for the analysis of complex mixtures because of its outstanding sensitivity. A huge amount of attention has been devoted to the miniaturization of portable MS instrumentation and to the development of direct sampling ionization 22 . Soft ionization methods such as matrix‐assisted laser desorption/ionization MS 23 and electrospray ionization MS 24 have been widely exploited for the analysis of biological samples 25,26 because of their high sensitivity and relatively fast operation time.…”