One-step hexaplex immunoassays by on-line paper substrate-based electrospray ionization mass spectrometry for combined cancer biomarker screening

Xu, Shuting; Liu, Mingxia; Feng, Jie; Yan, Gaoliang; Bai, Yu; Liu, Huwei

doi:10.1039/d0sc06784a

Cited by 19 publications

(24 citation statements)

References 48 publications

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“…It was possible to make the gold nanoparticles by reducing Au 3+ ions in aqueous glucose dispersions, and the efficient strategy was used according to the published. A 0.05 M solution of HAuCl 4 •3 H 2 O was added to a 0.03 M glucose solution to fabricate a conventional formulation [41][42][43]. To confirm that the reduction reaction had failed, the solution was agitated for more than 30 min and showed no sign of colour change.…”

Section: Fabrication Of Glucose-capped Gnps Loaded With Antimicrobial...mentioning

confidence: 99%

Facile construction of antibiotics-loaded glucose-capped gold nanoparticles for in vitro antimicrobial and treatment and care of severe Pneumonia

Shi

Liu

2023

Mater. Res. Express

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From the perspective of gold nanoparticles (GNPs)' potential antibacterial applications, we demonstrate the fabrication, characterization, and effective antimicrobial activity of gentamicin (GM) and kanamycin (KAN) dynamically loaded to glucose-capped gold nanoparticles (g-GNPs). Infra-red (FT-IR) spectroscopy analyzed the synthesized GNPs and g-GNPs with encapsulated antibiotic drugs. Various spectroscopical methods characterized g-GNPs and drug-loaded nanoparticles. A minimum inhibitory concentration (MIC) and active curves in the Klebsiella pneumonia strain were used to evaluate the antibacterial activity of aminoglycoside-loaded g-GNPs, and the results showed that the nanoparticles had an appropriate antimicrobial effect on the clinical strain of the bacteria. There were bacteriostatic effects and an inhibiting effect on the growth of bacteria at doses of 33 to 65 µg/ml for each GM@g-GNPs and KAN@g-GNPs. With zones of Inhibition (ZOI) of 27 and 29 mm, g-GNPs-loaded antimicrobial medications demonstrated more antibacterial activity in well diffusion experiments than free nanomaterials and antimicrobial drugs, with ZOI of 21.00 and 10.00 mm, respectively. GM and KAN-loaded g-GNPs were examined using crystal violet assay for their antibiofilm activity. Findings suggested that the concentration of nanoparticles and anti-biofilm activity were directly linked. The cell membrane integrity was assessed in g-GNPs loaded with GM and KAN, and the results showed that nucleic acids and proteins released into the environment were significant. The inhibitory effects of GM@g-GNPs and KAN@g-GNPs on bacterial efflux pump (EP) was assessed, and the result displayed that all strains were sensitive to moderate concentration of NPs and highly sensitive to concentrations of 0.6 and 0.9 µg/ml of ethidium bromide (EtBr) and 64 to 128 µg/ml of GM@g-GNPs and KAN@g-GNPs. The findings demonstrate that all strains were responsive to moderate nanoparticle concentrations.

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Section: Fabrication Of Glucose-capped Gnps Loaded With Antimicrobial...mentioning

confidence: 99%

Facile construction of antibiotics-loaded glucose-capped gold nanoparticles for in vitro antimicrobial and treatment and care of severe Pneumonia

Shi

Liu

2023

Mater. Res. Express

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“…Besides the mass tags of metal ions, nanoparticles, DNA barcodes, and peptides mentioned above, scientists have also tried to design, synthesize, or exploit mass-tag molecules of other species for mass spectrometric biosensing. This section summarizes the application examples of small molecules, − polyethylene glycol molecules (PEGs), − and phospholipids , as potential mass barcodes.…”

Section: Structure Of Mass-tag Probesmentioning

confidence: 99%

“…91 The LOD for thrombin was at zeptomole (Figure 7E), while three protein biomarkers (CA125, carcinoembryonic antigen, and epithelial cell adhesion molecule) could be simultaneously detected at cell numbers as low as 25 (Figure 7F). Owing to the satisfactory applicability of RMTs, a simple paper-based hexaplex immunoassay with an online MS readout was proposed, 92 and a multidimensional organic mass cytometry system was also established with a microfluidic chip connecting to MS, 93 both of which were proved to be promising for clinical diagnosis and therapy.…”

Section: ■ Structure Of Mass-tag Probesmentioning

confidence: 99%

Mass Spectrometric Biosensing: A Powerful Approach for Multiplexed Analysis of Clinical Biomolecules

Liu

Chen

et al. 2021

ACS Sens.

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Rapid and sensitive detection of clinical biomolecules in a multiplexed fashion is of great importance for accurate diagnosis of diseases. Mass spectrometric (MS) approaches are exceptionally suitable for clinical analysis due to its high throughput, high sensitivity, and reliable qualitative and quantitative capabilities. To break through the bottleneck of MS technique for detecting high-molecular-weight substances with low ionization efficiency, the concept of mass spectrometric biosensing has been put forward by adopting mass spectrometric chips to recognize the targets and mass spectrometry to detect the signals switched by the recognition. In this review, the principle of mass spectrometric sensing, the construction of different mass tags used for biosensing, and the typical combination mode of mass spectrometric imaging (MSI) technique are summarized. Future perspectives including the design of portable matching platforms, exploitation of novel mass tags, development of effective signal amplification strategies, and standardization of MSI methodologies are proposed to promote the advancements and practical applications of mass spectrometric biosensing.

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“…Alternatively, utilization of mass barcoded gold nanoparticles (AuNPs) affords the possibility to easily achieve multiplex assessment of targets by selecting mass tags (MTs) from the portfolio of commercially available chemicals (e.g., thiolated polyethylene glycol, single strand DNA, etc. ), − which possess a distinguishable mass fingerprint and high ionization efficiency for multiplex detection. , Furthermore, high-density MTs grafted on AuNPs facilitate signal amplification, which also allows wider compatibility to ionization techniques, typically laser desorption/ionization (LDI) and electrospray ionization (ESI). − Up until now, mass barcoded AuNPs have demonstrated prominent advantages in multiplex allergy diagnosis, in situ glycan detection, and protease activity assessment. − …”

mentioning

confidence: 99%

“…), 32−34 which possess a distinguishable mass fingerprint and high ionization efficiency for multiplex detection. 35,36 Furthermore, high-density MTs grafted on AuNPs facilitate signal amplification, which also allows wider compatibility to ionization techniques, typically laser desorption/ionization (LDI) and electrospray ionization (ESI). 37−40 Up until now, mass barcoded AuNPs have demonstrated prominent advantages in multiplex allergy diagnosis, in situ glycan detection, and protease activity assessment.…”

mentioning

confidence: 99%

Sense and Validate: Fluorophore/Mass Dual-Encoded Nanoprobes for Fluorescence Imaging and MS Quantification of Intracellular Multiple MicroRNAs

Zhang

Wang

et al. 2022

Anal. Chem.

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Simultaneously monitoring and quantifying intracellular multiple microRNAs (miRNAs) is highly essential to clinical diagnosis and pathological research. However, revealing the intracellular distribution of multiple miRNAs while determining their content in a multiplex and quantitative format remains challenging. Considering the respective technical merit of fluorescence imaging and mass spectrometry (MS) in in situ detection and multiplex assaying, we herein propose fluorophore/ mass dual-encoded nanoprobes (FMNPs) that can execute targettriggered hairpin self-assembly to enable in situ amplified imaging and follow-up MS quantification of intracellular multiple miRNAs. The FMNPs responsive to the target miRNA were constructed by codecorating gold nanoparticles (AuNPs) with locked hairpin DNA probes (LH1) and corresponding mass tags (MTs) for fluorescent and mass spectrometric dual-modal readout. Cellular miRNAs can separately trigger recycled hairpin self-assembly, leading to the continuous liberation of fluorophore-labeled bolt DNA (bDNA) for fluorescence imaging in cells. Moreover, the postreaction FMNPs afford an extra chance to validate the fluorescence output of miRNA-21 and miRNA-141 by accurate MS quantification relying on the ion signal of the barcoded MTs. Fluorescence imaging and MS quantification of miRNA-21 and miRNA-141 have also been successfully accomplished in different cell lines, highlighting its potential in cell subtyping. This "senseand-validate" strategy creates a new modality for assaying multiple intracellular miRNAs and holds great promise in unveiling multicomponent-involved events in cellular processes and determining multiple biomarkers in accurate clinical diagnosis.

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One-step hexaplex immunoassays by on-line paper substrate-based electrospray ionization mass spectrometry for combined cancer biomarker screening

Abstract: Mass spectrometry (MS) has been attractive for multiplexed immunoassay readout benefitting from its high sensitivity, speed and mass resolution. Here, a simple paper-based hexaplex immunoassay with on-line MS reatout was...

Cited by 19 publications

References 48 publications

Facile construction of antibiotics-loaded glucose-capped gold nanoparticles for in vitro antimicrobial and treatment and care of severe Pneumonia

Facile construction of antibiotics-loaded glucose-capped gold nanoparticles for in vitro antimicrobial and treatment and care of severe Pneumonia

Mass Spectrometric Biosensing: A Powerful Approach for Multiplexed Analysis of Clinical Biomolecules

Sense and Validate: Fluorophore/Mass Dual-Encoded Nanoprobes for Fluorescence Imaging and MS Quantification of Intracellular Multiple MicroRNAs

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