DIRECT STIMULATION OF BOVINE OVARIAN PROGESTERONE SECRETION BY LOW CONCENTRATIONS OF a-INTERFERON

Abstract

The ruminant conceptus secretes proteins during early pregnancy which maintain the corpus luteum. These trophoblast proteins are related to the alpha II-interferons and prevent luteolysis indirectly by disrupting the secretion of endometrial prostaglandin. Although trophoblast interferons appear to be largely confined to the uterine lumen, it remains possible that they also act peripherally. This report describes in vitro studies which suggest that interferon may influence hormone secretion by the ovary directly. The study employed i) a well defined serum-free culture model in which bovine granulosa cells secrete the luteal hormones progesterone and oxytocin, and ii) serum-free and serum-supplemented cultures of cells from early CL. Dose-response experiments were performed using bovine recombinant alpha-interferon (brIFN). Progesterone and oxytocin secretions were measured over 4-5 days of culture and DNA content was also determined. Low concentrations of brIFN (10(-15) mol/l to 10(-11) mol/l) stimulated progesterone secretion by granulosa cells by up to three fold, without significantly affecting oxytocin concentrations or culture DNA content. Concentrations of 10(-10) mol/l to 10(-1) mol/l suppressed progesterone secretion in a log dose-related manner (r = 0.97) with evidence of toxicity (lower oxytocin concentrations and significantly reduced DNA compared with controls). Progesterone secretion by luteal cells in serum-free culture was stimulated in the presence of 10(-15) mol/l brIFN, whilst high concentrations again caused inhibition. The data show that ovarian cells can respond directly to low concentrations of interferon-like proteins.(ABSTRACT TRUNCATED AT 250 WORDS)

Comparative effects of interferon alpha‐2b and pegylated interferon alpha‐2b on menstrual cycles and ovarian hormones in cynomolgus monkeys

“…Other reports also suggest that IFN interacts with the binding of a variety of compounds to their respective cell surface receptors, thereby modifying the function of different hormones (Kohn et al, 1976; Grollman et al, 1978; Blalock and Stanton, 1980). In ruminants, low doses of bovine IFNα (10 −15 mol/l) stimulated secretion of progesterone by granulosa and luteal cells; however, high doses (10 −10 mol/l) suppressed progesterone secretion (Luck et al, 1992). This suggests that ovarian cells can respond directly to IFN‐like proteins, and the mechanism by which bovine IFNα causes reduction in plasma progesterone is probably due to interference with pituitary support for luteal synthesis of progesterone (Barros et al, 1992).…”

Bovine Embryonic Mortality with Special Reference to Mineral Deficiency, Heat Stress and Endocrine Factors: A Review

“…The mechanism of action of IFN may involve suppression of both oestradiol and oxytocin receptors probably at the transcriptional level (Bazer et al, 1994). Bovine Interferonalpha (bIFNα) has been shown to stimulate progesterone production by luteal cells in vitro, without affecting oxytocin output (Luck et al, 1992). Imakawa et al (1993) reported that maternal granulocyte macrophage colony stimulating factor might be involved in stimulating embryo IFN production.…”