Direct structural evidence supporting a revolving mechanism in DNA packaging motors

Shu, Yeqiang; Cheng, Xiaolin

doi:10.1007/s41048-020-00115-w

Cited by 1 publication

(1 citation statement)

References 25 publications

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“…As a result, the molecules are inevitably exposed to the air at a high surface-to-volume ratio. The air-water interface (AWI) has been considered unfavorable for proteins to maintain their native conformation [70][71][72][73]. Protein denaturation due to the AWI can happen within milliseconds or even less [71,72,[74][75][76], which can be summarized in three stages [77]: first, protein adsorbs to the AWI, followed by shearing stress disrupts the protein structure and the polypeptide chains unfolding to form inactive aggregates [78].…”

Section: Prevention Of Protein Denaturation Caused By Air-water Interfacementioning

confidence: 99%

Application of Monolayer Graphene and Its Derivative in Cryo-EM Sample Preparation

Zhu

et al. 2021

IJMS

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Cryo-electron microscopy (Cryo-EM) has become a routine technology for resolving the structure of biological macromolecules due to the resolution revolution in recent years. The specimens are typically prepared in a very thin layer of vitrified ice suspending in the holes of the perforated amorphous carbon film. However, the samples prepared by directly applying to the conventional support membranes may suffer from partial or complete denaturation caused by sticking to the air–water interface (AWI). With the application in materials, graphene has also been used recently to improve frozen sample preparation instead of a suspended conventional amorphous thin carbon. It has been proven that graphene or graphene oxide and various chemical modifications on its surface can effectively prevent particles from adsorbing to the AWI, which improves the dispersion, adsorbed number, and orientation preference of frozen particles in the ice layer. Their excellent properties and thinner thickness can significantly reduce the background noise, allowing high-resolution three-dimensional reconstructions using a minimum data set.

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Section: Prevention Of Protein Denaturation Caused By Air-water Interfacementioning

confidence: 99%

Application of Monolayer Graphene and Its Derivative in Cryo-EM Sample Preparation

Zhu

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

Direct structural evidence supporting a revolving mechanism in DNA packaging motors

Cited by 1 publication

References 25 publications

Application of Monolayer Graphene and Its Derivative in Cryo-EM Sample Preparation

Application of Monolayer Graphene and Its Derivative in Cryo-EM Sample Preparation

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