Direct Tension Recording From Smooth Muscle of Resistance Vessels From Various Organs

Bohr, David F.; Goulet, Patricia L.; Taquini, Alberto C.

doi:10.1177/000331976101201009

Cited by 132 publications

(38 citation statements)

References 5 publications

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“…In canine basilar arteries, 5-HT induced the strongest vasoconstriction, and norepinephrine did not show significant constriction which has been already reported by lots of investigators (Bohr et al 1961;Ersparmer 1966 ;Nielsen and Owman 1971). Swank and Hissen (1964) reported that 5-HT induced an increase of blood flow rate in the canine brain in vivo.…”

Section: Monkey Basilar Arterysupporting

confidence: 56%

Vascular responsiveness of isolated, perfused basilar arteries in dogs and monkeys.

Chiba

Tsuji

1985

Tohoku J. Exp. Med.

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A new model of isolated basilar arterial preparation was developed in the dog and monkey. Isolated arteries were perfused at a constant flow rate with Krebs solution and suspended in a bath at 3TC. By inserting the steel cannula into the artery, the space between the luminal wall of the artery and the cannula was narrowed enough to obtain a suitable perfusion pressure during the perfusion. The resting perfusion pressure was maintained at a constant level of 50-100 mmHg. The drug solution was intraluminally administered, and the response was obtained as changes in the perfusion pressure. Responses to 6 vasoactive substances (norepinephrine, 5-HT, PGF2a, histamine, ATP and KCl) were compared between simian and canine basilar arteries. It was demonstrated that in simian arteries, PGF2a was the strongest but 5-HT and norepinephrine induced slight vasoconstriction to the same degree, and in canine basilar arteries, 5-HT induced the strongest constriction but norepinephrine did not produce significant vasoconstriction, and KC1 induced marked constriction to the same degree at extremely large doses in both species. It was demonstrated that the cannula inserting method is useful to observe the responses of isolated basilar arteries. cannula inserting method ; isolated basilar artery ; simian cerebral artery ; dog cerebral artery Recently, a new model of isolated and perfused vascular preparation was developed by Hongo and Chiba (1983) and modified by Tsuji and Chiba (1984). In their preparation, the resting perfusion pressure was maintained between 50-100 mm Hg which was close to physiological arterial blood pressure throughout the experiment. The drug administration was performed on the endothelial side of the artery. By using this method, vascular responses to vasoactive substances were studied in several parts of relatively large arteries isolated (Hongo and Chiba 1983;Tsuji and Chiba 1984; Chiba and Tsukada 1984a, b ; Ito and Chiba 1984a, b). In the present experiments, we made an attempt to apply this method to the isolated cerebral artery in the dog and monkey, by using a modified cannula inserting method, and to compare effects of 6 different vasoactive substances

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Section: Monkey Basilar Arterysupporting

confidence: 56%

Vascular responsiveness of isolated, perfused basilar arteries in dogs and monkeys.

Chiba

Tsuji

1985

Tohoku J. Exp. Med.

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“…Regional differences in the response of isolated canine arteries of the dog to vasoconstrictor and dilator agents have been demonstrated (Bohr, Goulet & Taquini, 1961;Toda & Fujita, 1973;Toda, 1974). The relaxant effect of dopamine in different arteries under the same experimental conditions are summarized in Figure 5.…”

Section: Discussionmentioning

confidence: 99%

Influence of Dopamine and Noradrenaline on Isolated Cerebral Arteries of the Dog

Toda

1976

British J Pharmacology

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I Effects of dopamine and noradrenaline were compared in helically-cut strips of canine cerebral arteries.2 Dopamine caused a greater maximal contraction than noradrenaline, although the ED50 for noradrenaline was appreciably less. The contraction induced by these amines was reversed to a relaxation by treatment with phenoxybenzamine. 3 Relaxation induced by dopamine in phenoxybenzamine-treated and prostaglandin-contracted cerebral arteries was not influenced by 1 gM propranolol, while relaxation induced by noradrenaline at low concentrations (2 gM and 10 gM) was significantly attenuated. Neither aminophylline nor atropine affected the relaxant effect of dopamine.4 A mechanism other than P-adrenergic, cholinergic or adenosine-related appears to be involved in the relaxation elicited by dopamine in cerebral arterial strips.

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“…A detailed knowledge of the adrenergic receptor mechanisms mediating the vasomotor responses of the brain vessels is a prerequisite for a proper understanding of the physiological role of sympathetic nerves and circulating catecholamines in the regulation of cerebral hemodynamics. Although such information is available for several peripheral vascular beds (9), little is known about cerebrovascular adrenoceptors, perhaps partly because early investigations failed to obtain catecholamine-induced vasomotor responses in isolated cerebral resistance vessels (10). However, using a sensitive in vitro system (11), Nielsen and Owman (12) have been able to record strong contractions induced by 835…”

mentioning

confidence: 99%

Pharmacological Characterization of Adrenergic Alpha and Beta Receptors Mediating the Vasomotor Responses of Cerebral Arteries Ir Vitro

Edvinsson

Owman

1974

Circulation Research

215

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The adrenergic receptors in the isolated feline middle cerebral artery were characterized pharmacologically using a sensitive system for recording circular contractions in vitro. Epinephrine, norepinephrine, isoproterenol. and phenylephrine contracted the vessel in the mentioned order of potency. Together with the inhibitory patterns obtained with graded doses of piperoxan (reversible competitive inhibition) and dibenamine or phenoxybenzamine (irreversible competitive inhibition), these results showed that the contraction was mediated by alpha receptors. With piperoxan and norepinephrine, the mean value for pA, was 7.06 and for K H 1.24 > 10 7 M. The mean value for K A calculated for norepinephrine before and after partial irreversible blockade of the alpha receptors with phenoxybenzamine was 1.73 x 10"M. The norepinephrine response was not directly proportional to the amount of receptors occupied; ED 60 was reached when only about 11*7 of the receptors were occupied and the E Am response was obtained when 75 r 7 of the receptors were occupied. Dilation was achieved only after an active tonic contraction had been induced (with 5-hydroxytryptamine) in the vessels, and the order of potency was isoproterenol > norepinephrine = epinephrine > terbutaline. Inhibition with INPEA and propranolol was competitive, as confirmed by Arunlakshana-Schild plots, showing that the dilatory response was a beta-receptor effect. The values for pA, (8.78 and 9.17) and K B (2.31 x 10~"M and 1.77 x 10~*M) for propranolol were indistinguishable in tests with terbutaline and isoproterenol, respectively. Comparison of the relative potencies of norepinephrine and epinephrine as well as isoproterenol and terbutaline suggested that the receptors were of the beta, type.

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Direct Tension Recording From Smooth Muscle of Resistance Vessels From Various Organs

Cited by 132 publications

References 5 publications

Vascular responsiveness of isolated, perfused basilar arteries in dogs and monkeys.

Vascular responsiveness of isolated, perfused basilar arteries in dogs and monkeys.

Influence of Dopamine and Noradrenaline on Isolated Cerebral Arteries of the Dog

Pharmacological Characterization of Adrenergic Alpha and Beta Receptors Mediating the Vasomotor Responses of Cerebral Arteries Ir Vitro

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