Direct visualization and measurements of wound neovascularization: Application in microsurgery research

Kjølseth, Dorthe; Mk, Kim; Lh, Andresen; Jm, Frank; Schuschke, Dale A.; Gl, Anderson; Jc, Banis; Gr, Tobin; Lj, Weiner

doi:10.1002/micr.1920150606

Cited by 17 publications

(17 citation statements)

References 35 publications

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“…As these results suggested that the MEST-encoded protein may be involved in the process of neoangiogenesis that occurs during tumour formation, Mest expression was assessed during wound healing of murine skin. Although neoangiogenesis occurs during wound healing (Kjolseth et al, 1994;Thompson et al, 1991), Mest transcripts could not be detected in sections of skin undergoing healing (not shown).…”

Section: Mest/mest Expression In Choriocarcinoma Grown In Nude Micementioning

confidence: 57%

Expression of the imprinted genesMEST/Mest in human and murine placenta suggests a role in angiogenesis

et al. 2000

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Section: Mest/mest Expression In Choriocarcinoma Grown In Nude Micementioning

confidence: 57%

Expression of the imprinted genesMEST/Mest in human and murine placenta suggests a role in angiogenesis

et al. 2000

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“…Consequently, the effects of many factors on angiogenesis still remain unknown. [6][7][8] The increase in total blood vessel length at 32% and 63% after fibroblast growth factor (FGF) and TGF-injections applied for 20 days indicates that FGF and TGF-assume the main role as the progenitor of endothelial cells in angiogenesis. 16,17 This study has demonstrated the epidermal growth factor increases the degree of healing in connective tissue but it does not affect angiogenesis, as previously assumed.…”

Section: Resultsmentioning

confidence: 98%

The Effects of Melatonin on Angiogenesis and Wound Healing

et al. 2003

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“…The ear of the homozygous hairless mouse measures about 13 mm 2 . The total thickness is approximately 300 µm with a central cartilage layer between two layers of skin [3,4,9,11,18,19]. All handling of the animals was performed under anesthesia (ketamine 50 mg/kg and xylazine hydrochloride 5 mg/kg i.p.).…”

Section: Animal Modelmentioning

confidence: 99%

In Vivo Effect of Tumor Necrosis Factor Alpha on Wound Angiogenesis and Epithelialization

Frank

Born²,

Barker

et al. 2003

European Journal of Trauma

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Background: The role of tumor necrosis factor alpha (TNF-␣) in wound healing is unclear and the results are contradictory. In vivo, TNF-␣ induces vessel growth, an important step in promoting wound healing. However, a reduced amount of collagen, hydroxyproline, and granulation tissue was found after TNF-␣ treatment. It is also unknown if this is a direct effect, by influencing cells involved in wound healing, or an indirect effect due to a negative or positive effect on cells such as macrophages. Material and Methods: The current study was undertaken to test the effect of TNF-␣ on wound epithelialization and neovascularization in vivo. In the first experiment, standardized full-thickness dermal wounds (2.25 mm diameter, 0.125 mm depth) were created on the dorsum of the ears of male hairless mice. The wounds were treated either with TNF-␣ (100 ng/ml, 1 µg/ml, 5 µg/ml; n = 10 per group), monoclonal TNF-␣ antibody (10 µg/ml; n = 10), or vehicle (n = 10). Wound epithelialization and neovascularization were analyzed every 3rd day by intravital microscopy until complete healing. In a second experiment, the same wound model was used, but in order to impair the healing process, macrophages were depleted. To reduce macrophages, two out of four groups (n = 10 per group) were pretreated with iota-carrageenan (MR groups), and the other two groups received only saline (N groups). One N group and one MR group were treated with TNF-␣ (1 µg/ml). The other N group and MR group received vehicle only (carboxymethylcellulose). Using intravital microscopy and computerized planimetry, wound epithelialization and neovascularization were measured every 3rd day until complete healing. Immunohistochemistry was performed to detect TNF-␣, macrophages, fibronectin, and vitronectin receptors. Results: In the first experiment the wounds treated with 1 µg/ml healed significantly earlier than controls (13.9 ± 2 vs. 17.3 ± 2.8 days, respectively; p < 0.05). Epithelialization in the antibody group was significantly slower compared to controls (20.1 ± 2 days; p < 0.05). Neovascularization was significantly enhanced in the group treated with 1 µg/ml TNF-␣ (p < 0.05). In the second experiment the wounds treated with TNF-␣ were significantly earlier epithelialized (13.1 ± 0.6) and vascularized (16.0 ± 0.5) compared to controls (16.8 ± 0.4 vs. 17.6 ± 0.5; p < 0.05). Wound closure was significantly delayed in the MR group treated with vehicle only (20.4 ± 1) and equal to controls in the MR group treated with TNF-␣ (16.8 ± 0.6). Conclusion:The results demonstrate the TNF-␣ accelerates wound epithelialization and neovascularization in this in vivo model. TNF-␣ is able to compensate for the negative effect of macrophage reduction and seems to have a direct effect on the wound-healing process.

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Direct visualization and measurements of wound neovascularization: Application in microsurgery research

Cited by 17 publications

References 35 publications

Expression of the imprinted genesMEST/Mest in human and murine placenta suggests a role in angiogenesis

Expression of the imprinted genesMEST/Mest in human and murine placenta suggests a role in angiogenesis

The Effects of Melatonin on Angiogenesis and Wound Healing

In Vivo Effect of Tumor Necrosis Factor Alpha on Wound Angiogenesis and Epithelialization

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