Direct visualization of the protein corona using carbon nanodots as a specific contrasting agent

Rao, Chethana; Yadav, Aditya; Kaur, Rimanpreet; Prasad, Amit; Nandi, Chayan Kanti

doi:10.1039/d0cc06333a

Cited by 3 publications

(3 citation statements)

References 22 publications

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“…This is because the use of nonspecific heavy metals to increase protein contrast in the TEM method may disrupt the secondary structure of the proteins, leading to artificial and erroneous conclusions. In recent years, a new strategy using a small (size 4 nm) nontoxic biocompatible fluorescent cariogenic nanodot (FND) was developed [ 97 ] for more precise quantification of nanoparticle size and visualization of the protein corona at a higher resolution (Figure 7b).…”

Section: Detection and Characterization Of Protein Coronasmentioning

confidence: 99%

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Characterization and Biomedical Application Opportunities of the Nanoparticle's Protein Corona

Jiang

2022

Adv Materials Inter

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with all three external dimensions in the nanoscale, whose longest and shortest axes do not differ significantly, with a significant difference typically being a factor of at least 3." This size allows nanoscale materials to be integrated into biomedical equipment since most biological systems are also nanoscale. [2] In addition to the definition of size, NPs used in biomedical research have a high degree of diversity in structure, chemical composition, morphology, hydrophobicity, surface chemistry, electrostatic charge, and other properties. Figure 1 shows several typical NPs (magnetic NPs, carbon nanotubes, liposomes, micelles, etc.) and the related phenomena in biomedicine. [3][4][5][6][7] Various nanotechnology platforms based on these NPs have been well developed. [8][9][10][11][12] The first-generation nanocarriers, e.g., polymeric NPs, polyethylene glycol (PEG)-modified liposomes, can improve the pharmacokinetics and tolerability of nanoformulated drugs. [13,14] Magnetic materials are attractive for applications in biomedicine, such as directly focusing the nanocarriers on the target area by an external magnetic field [15][16][17] or killing the cancerous cells through magnetically induced hyperthermia. [18,19] Moreover, the drugs for central nervous system diseases must cross the blood-brain barrier. The nanocarriers can specifically deliver the payloads to affected regions and accumulate in selected tissues, [20][21][22] assisting in solubilizing the compounds with low solubility (e.g., paclitaxel and atovaquone) [23] and protecting the molecules of proteins and/or nucleic acids from enzymatic degradation. The next-generation nanocarriers take advantage of active targeting methods to ensure drug delivery to specific areas. These strategies depend on the surface functionalization of nanocarriers with active targeting molecules, [24] for example, hyaluronic acid, [25] folic acid, [26] antibodies, [27] or aptamers. [28] For example, Chan and collaborators developed the DNA-assembled gold nanorod "core-satellite" structures for programmable drug loading and controllable release. [29] Moreover, researchers explored the functionality of DNA-assembled inorganic NPs for operating in the complicated biological systems. It was revealed that altering the targeting ligands on the surface increased the targeting efficiency by 2.5 times. [30] Therefore, NPs have a strong potential to increase the stability and solubility of the encapsulated drugs, and promote drug transport across membranes, as well as prolong the drug circulation times and halflife to improve the safety and efficacy of drugs. [31,32] Nanoparticles (NPs) are widely used in a variety of biomedical fields, such as drug delivery systems, novel theragnostic strategies, bioimaging, and biosensing. The protein corona formed by the contact between NPs and biological liquids changes the physicochemical nanoparticle characteristics, including size, shape, and surface statement. Moreover, it affects the biological fate of NPs in organisms, for instance, pharmac...

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Section: Detection and Characterization Of Protein Coronasmentioning

confidence: 99%

Section: Detection and Characterization Of Protein Coronasmentioning

confidence: 99%

Characterization and Biomedical Application Opportunities of the Nanoparticle's Protein Corona

Jiang

2022

Adv Materials Inter

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“…23 Based on the AI-Cz platform, we recently developed various of TADF probes and successfully utilized them as imaging tools for the understanding of bacterial biology. 24–26 In this work, we expanded the thionation approach to TADF molecules on the basis of the AI-Cz platform to provide a novel strategy for developing potential PSs for bacterial imaging and disinfection (Scheme 1). This new class of thio-based and heavy-atom free PSs could be obtained by performing a simple sulfur substitution of carbonyl oxygen atoms of the biocompatible TADF emitters.…”

Section: Introductionmentioning

confidence: 99%

Thermally activated delayed fluorescence emitters: a thionation approach toward next-generation photosensitizers

et al. 2023

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Complexation of turmeric and curcumin mediated silver nanoparticles with human serum albumin: Further investigation into the protein-corona formation, anti-bacterial effects and cell cytotoxicity studies

Baruah

Konthoujam

Lyndem

et al. 2023

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Direct visualization of the protein corona using carbon nanodots as a specific contrasting agent

Abstract: A specific labeling strategy using carbon dots helped in the direct visualization of protein corona under TEM.

Cited by 3 publications

References 22 publications

Characterization and Biomedical Application Opportunities of the Nanoparticle's Protein Corona

Characterization and Biomedical Application Opportunities of the Nanoparticle's Protein Corona

Thermally activated delayed fluorescence emitters: a thionation approach toward next-generation photosensitizers

Complexation of turmeric and curcumin mediated silver nanoparticles with human serum albumin: Further investigation into the protein-corona formation, anti-bacterial effects and cell cytotoxicity studies

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