The drag reduction design of underwater vehicles is of great significance to saving energy and enhancing speed. In this paper, the drag reduction characteristics of Paramisgurnusdabryanus loach was explored using 3D ultra-depth field microscopy to observe the arrangement of the scales. Then, a geometric model was established and parameterized. A simulated sample was processed by computer numerical control (CNC) machining and tested through using a flow channel bench. The pressure drop data were collected by sensors, and the drag reduction rate was consequently calculated. The test results showed that the drag reduction rate of a single sample could reach 23% at a speed of 1.683 m/s. Finally, the experimental results were verified by numerical simulation and the drag reduction mechanism was explored. The boundary layer theory and RNG k-ε turbulence model were adopted to analyze the velocity contour, pressure contour and shear force contour diagrams. The numerical simulation results showed that a drag reduction effect could be achieved by simulating the microstructure of scales of the Paramisgurnusdabryanus loach, showing that the results are consistent with the flow channel experiment and can reveal the drag reduction mechanism. The bionic surface can increase the thickness of boundary layer, reduce the Reynolds number and wall resistance. The scales disposition of Paramisgurnusdabryanus loach can effectively reduce the surface friction, providing a reference for future research on drag reduction of underwater vehicles such as ships and submarines.