Disadaptive genetic-evolutionary processes in human populations of industrial cities

Artemenkov, Aleksey A.

doi:10.23888/pavlovj2020282234-248

“…Numerous studies have indicated that the atmospheric air of large industrial cities annually receives about 500 thousand tons of harmful substances (about 450 names) with dust and gas emissions, while the volume of emissions of pollutants from stationary sources of pollution and vehicle exhaust gases is comparable to each other [16].…”

Section: Introductionmentioning

confidence: 99%

Chromosomal anomalies of the fetus of pregnant women from settlements prone to technogenic pollution

Kalimagambetov

¹

,

Sarsenovа

²

,

Жумагулова

³

et al. 2021

EJE

0

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The article presents the analysis results of the spectrum and frequency of distribution of the fetus chromosomal set of 590 pregnant women living in the city of Aktobe and various districts of the Aktobe region in Kazakhstan. Biological material was obtained by biopsy of the chorion and placenta, amniocentesis and cordocentesis. For the preparation of chromosome formations, the conventional "direct" method and the classical method of cell cultivation were used. Metaphase cells were stained with GTG and FISH methods. In total, 64 (10.9%) cases of fetal karyotype abnormalities were detected. Of these, genomic abnormalities accounted for 59 (10.0%), and structural -5 (8.5%). Among the genomic abnormalities in 39 (66.1%) cases, the fetus had a Down syndrome karyotype. The frequency of chromosomal abnormalities in the fetus of pregnant women in Aktobe, where industrial facilities of ferrous and nonferrous metallurgy are located, was 7.9%. The frequency of fetal chromosomal abnormalities in 3 districts of Aktobe region (fetal karyotypes of 39 pregnant women were studied) without large industrial facilities was 12.8%, and with the presence of large industrial complexes in 5 districts (fetal karyotypes of 131 pregnant women were studied) -19,8%, which is 1.5 times more. Thus, it can be assumed that technogenic pollution by large industrial complexes (mining, cement, chemical, oil and gas processing industries) of places of residence contributes to the appearance of genomic and structural disorders of the genetic apparatus of human cells.

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“…It should be noted that polymorphisms of candidate genes responsible for the immune regulation make a significant contribution to formation of a specific immune profile, which plays an important role in adaptation to changed environmental conditions [6,7]. Changes in an immune profile associated with polymorphisms of the tumor suppressing gene р53 TP53 (rs1042522) can be related to dysregulation of the cell cycle, DNA repair and apoptosis and growing risks of oncoproliferative states.…”

mentioning

confidence: 99%

Polymorphism of TP53 (rs1042522) gene and peculiarities of the immune profile in children exposed to airborne benzo(a)pyrene

Dolgikh,

Nikonoshina

2024

Health Risk Analysis

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Examining peculiarities of an immune profile and genetic polymorphisms is especially relevant when identifying markers of effect and sensitivity to exposure to benzo(a)pyrene in northern areas. We examined 1253 children who lived in industrial centers and on conditionally clean territories in the north and south of Eastern Siberia. Levels of benzo(a)pyrene in ambient air and in children’s blood were determined by using HPLC. TP53 (rs1042522) polymorphism was examined by using real-time PCR; p53 levels were identified with flow cytofluorometry; IgG to benzo(a)pyrene, by the radioallergosorbent tests. Exposure of children in Northern Siberia to airborne benzo(a)pyrene at the dose of 7.11•10-3 µg/(kg•day) causes ben-zo(a)pyrene contamination in blood, activates apoptosis (p53) and stimulates occurrence of specific sensitization (IgG to benzo(a)pyrene) (p < 0.05). Similar disorders were established in children in Southern Siberia under exposure to airborne benzo(a)pyrene at the dose of 86.46•10-3 µg/(kg•day). The detected changes in immune profiles of children living in Northern Siberia are associated with G-allele and GG-genotype (rs1042522) of the TP53 gene (OR = 1.37–1.83, p < 0.05); children in Southern Siberia, С-allele and СС-genotype of the said gene (OR = 1.55–2.38, p < 0.05). Therefore, the immune profile of children exposed to airborne benzo(a)pyrene at the dose of 7.11•10-3 µg/(kg•day) in Northern Siberia bears some signs of activated apoptosis (p53) and specific sensitization (IgG to benzo(a)pyrene) associated with G-allele and GG-genotype of the TP53 gene (rs1042522) (OR = 1.37–1.83, p < 0.05). These identified changes in the immune profile are comparable with effects produced by exposure to airborne benzo(a)pyrene at the dose of 86.46•10-3 µg/(kg•day) in Southern Siberia, which are associated with С-allele and СС-genotype of the same gene (OR = 1.55–2.38, p < 0.05). This confirms a hypothesis that effects of technogenic chemical factors can be modulated by specific climatic conditions in northern areas and a contribution made by genetic predisposition. Their combined effect creates a serious risk of developing impairments in an immune profile (OR = 1.37–1.83, p < 0.05; RR = 1.17; 95 % CI: 1.07–1.27) even under low-dose exposure to airborne benzo(a)pyrene.

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“…Важно отметить, что полиморфизм кандидатных генов иммунной регуляции вносит весомый вклад в формирование особого иммунного профиля, играющего важную роль в адаптации к измененным условиям окружающей среды [6,7]. Изменения иммунного профиля, ассоциированные с полиморфными вариантами гена белка-онкосупрессора р53 TP53 (rs1042522), могут быть ассоциированы с нарушениями регуляции клеточного цикла, репарации ДНК и апоптоза и повышением риска развития онкопролиферативных состояний, что особо актуально в условиях воздействия бенз(а)пирена как канцерогенного химического фактора [8,9].…”

unclassified

Polymorphism of TP53 (rs1042522) gene and peculiarities of the immune profile in children exposed to airborne benzo(a)pyrene

Dolgikh,

Nikonoshina

2024

Health Risk Analysis

0

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Examining peculiarities of an immune profile and genetic polymorphisms is especially relevant when identifying markers of effect and sensitivity to exposure to benzo(a)pyrene in northern areas. We examined 1253 children who lived in industrial centers and on conditionally clean territories in the north and south of Eastern Siberia. Levels of benzo(a)pyrene in ambient air and in children’s blood were determined by using HPLC. TP53 (rs1042522) polymorphism was examined by using real-time PCR; p53 levels were identified with flow cytofluorometry; IgG to benzo(a)pyrene, by the radioallergosorbent tests. Exposure of children in Northern Siberia to airborne benzo(a)pyrene at the dose of 7.11•10-3 µg/(kg•day) causes ben-zo(a)pyrene contamination in blood, activates apoptosis (p53) and stimulates occurrence of specific sensitization (IgG to benzo(a)pyrene) (p < 0.05). Similar disorders were established in children in Southern Siberia under exposure to airborne benzo(a)pyrene at the dose of 86.46•10-3 µg/(kg•day). The detected changes in immune profiles of children living in Northern Siberia are associated with G-allele and GG-genotype (rs1042522) of the TP53 gene (OR = 1.37–1.83, p < 0.05); children in Southern Siberia, С-allele and СС-genotype of the said gene (OR = 1.55–2.38, p < 0.05). Therefore, the immune profile of children exposed to airborne benzo(a)pyrene at the dose of 7.11•10-3 µg/(kg•day) in Northern Siberia bears some signs of activated apoptosis (p53) and specific sensitization (IgG to benzo(a)pyrene) associated with G-allele and GG-genotype of the TP53 gene (rs1042522) (OR = 1.37–1.83, p < 0.05). These identified changes in the immune profile are comparable with effects produced by exposure to airborne benzo(a)pyrene at the dose of 86.46•10-3 µg/(kg•day) in Southern Siberia, which are associated with С-allele and СС-genotype of the same gene (OR = 1.55–2.38, p < 0.05). This confirms a hypothesis that effects of technogenic chemical factors can be modulated by specific climatic conditions in northern areas and a contribution made by genetic predisposition. Their combined effect creates a serious risk of developing impairments in an immune profile (OR = 1.37–1.83, p < 0.05; RR = 1.17; 95 % CI: 1.07–1.27) even under low-dose exposure to airborne benzo(a)pyrene.

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Disadaptive genetic-evolutionary processes in human populations of industrial cities

Cited by 6 publications

References 24 publications

Chromosomal anomalies of the fetus of pregnant women from settlements prone to technogenic pollution

Chromosomal anomalies of the fetus of pregnant women from settlements prone to technogenic pollution

Polymorphism of TP53 (rs1042522) gene and peculiarities of the immune profile in children exposed to airborne benzo(a)pyrene

Polymorphism of TP53 (rs1042522) gene and peculiarities of the immune profile in children exposed to airborne benzo(a)pyrene

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