Discerning different and opposite effects of hydrogenase on the corrosion of mild steel in the presence of phosphate species

Mehanna, Maha; Rouvre, Ingrid; Délia, Marie-Line; Féron, Damien; Bergel, Alain; Basseguy, Régine

doi:10.1016/j.bioelechem.2016.04.005

Cited by 9 publications

(6 citation statements)

References 59 publications

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“…The latter is plausible because several acetogens can grow on electrodes poised at potentials that do not generate abiotic H 2 [60–62]. On the other hand, previous studies illustrated efficient enzymatic-mediated electron uptake from Fe 0 using a purified Clostridium [FeFe]-hydrogenase, which retrieves electrons directly from Fe 0 for proton oxidation to H 2 [36, 37]. Unlike [NiFe]-hydrogenases from methanogens, the [FeFe]-hydrogenases of Clostridium are effective at oxidizing H + [63] and quickly evolving H 2 that could serve as an electron donor for Baltic-acetogens.…”

Section: Resultsmentioning

confidence: 99%

“…It is not clear whether an extracellular enzyme-dependent strategy would be competitive in corrosive environments. Moribund cells may release enzymes like hydrogenases into their extracellular milieu [19] that can capture electrons freed during Fe 0 -oxidation to reduce protons from solution to H 2 [36, 37]. H 2 could then be used non-specifically by a variety of H 2 -utilizers.…”

Section: Introductionmentioning

confidence: 99%

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Baltic Sea methanogens compete with acetogens for electrons from metallic iron

et al. 2019

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Microbially induced corrosion of metallic iron (Fe0)-containing structures is an environmental and economic hazard. Methanogens are abundant in low-sulfide environments and yet their specific role in Fe0 corrosion is poorly understood. In this study, Sporomusa and Methanosarcina dominated enrichments from Baltic Sea methanogenic sediments that were established with Fe0 as the sole electron donor and CO2 as the electron acceptor. The Baltic-Sporomusa was phylogenetically affiliated to the electroactive acetogen S. silvacetica. Baltic-Sporomusa adjusted rapidly to growth on H2. On Fe0, spent filtrate enhanced growth of this acetogen suggesting that it was using endogenous enzymes to retrieve electrons and produce acetate. Previous studies have proposed that acetate produced by acetogens can feed commensal acetoclastic methanogens such as Methanosarcina. However, Baltic-methanogens could not generate methane from acetate, plus the decrease or absence of acetogens stimulated their growth. The decrease in numbers of Sporomusa was concurrent with an upsurge in Methanosarcina and increased methane production, suggesting that methanogens compete with acetogens for electrons from Fe0. Furthermore, Baltic-methanogens were unable to use H2 (1.5 atm) for methanogenesis and were inhibited by spent filtrate additions, indicating that enzymatically produced H2 is not a favorable electron donor. We hypothesize that Baltic-methanogens retrieve electrons from Fe0 via a yet enigmatic direct electron uptake mechanism.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Baltic Sea methanogens compete with acetogens for electrons from metallic iron

et al. 2019

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“…Acetogens often dominate corrosive communities, outcompeting methanogens when concentrations of H 2 are high and temperatures are low, presumably due to the higher kinetics ( V max ) of their hydrogenases ( Kotsyurbenko et al, 2001 ). Unlike methanogens, acetogens contain [FeFe]-hydrogenases ( Peters et al, 2015 ), which could retrieve electrons directly from Fe 0 for proton reduction to H 2 ( Mehanna et al, 2008 , 2016 ; Rouvre and Basseguy, 2016 ). In this study, we were interested to understand the interspecies dynamics on Fe 0 between acetogens and methanogens from a climate lake.…”

Section: Introductionmentioning

confidence: 99%

A Win–Loss Interaction on Fe0 Between Methanogens and Acetogens From a Climate Lake

2021

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Diverse physiological groups congregate into environmental corrosive biofilms, yet the interspecies interactions between these corrosive physiological groups are seldom examined. We, therefore, explored Fe0-dependent cross-group interactions between acetogens and methanogens from lake sediments. On Fe0, acetogens were more corrosive and metabolically active when decoupled from methanogens, whereas methanogens were more metabolically active when coupled with acetogens. This suggests an opportunistic (win–loss) interaction on Fe0 between acetogens (loss) and methanogens (win). Clostridia and Methanobacterium were the major candidates doing acetogenesis and methanogenesis after four transfers (metagenome sequencing) and the only groups detected after 11 transfers (amplicon sequencing) on Fe0. Since abiotic H2 failed to explain the high metabolic rates on Fe0, we examined whether cell exudates (spent media filtrate) promoted the H2-evolving reaction on Fe0 above abiotic controls. Undeniably, spent media filtrate generated three- to four-fold more H2 than abiotic controls, which could be partly explained by thermolabile enzymes and partly by non-thermolabile constituents released by cells. Next, we examined the metagenome for candidate enzymes/shuttles that could catalyze H2 evolution from Fe0 and found candidate H2-evolving hydrogenases and an almost complete pathway for flavin biosynthesis in Clostridium. Clostridial ferredoxin-dependent [FeFe]-hydrogenases may be catalyzing the H2-evolving reaction on Fe0, explaining the significant H2 evolved by spent media exposed to Fe0. It is typical of Clostridia to secrete enzymes and other small molecules for lytic purposes. Here, they may secrete such molecules to enhance their own electron uptake from extracellular electron donors but indirectly make their H2-consuming neighbors—Methanobacterium—fare five times better in their presence. The particular enzymes and constituents promoting H2 evolution from Fe0 remain to be determined. However, we postulate that in a static environment like corrosive crust biofilms in lake sediments, less corrosive methanogens like Methanobacterium could extend corrosion long after acetogenesis ceased, by exploiting the constituents secreted by acetogens.

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“…In the Baltic corrosive community Clostridium -species are likely to play the role of acetogens, since several species have been shown to produce acetate either by electrosynthesis of autotrophically on H 2 [50, 58, 59]. Besides, clostridial enzymes, for example [Fe]-hydrogenases from Clostridium pasteurianium, were shown capable of corrosion on their own, assumedly by direct retrieval of electrons coupled to proton reduction to H 2 [60,61]. If a sacrificial population exuded enzymes, then the addition of spent-media filtrate, expected to contain exuded enzymes, would significantly stimulate H 2 -production and subsequent H 2 -uptake by Baltic-acetogens.…”

Section: Resultsmentioning

confidence: 99%

BalticMethanosarcinaandClostridiumcompete for electrons from metallic iron

Snoeyenbos-West

Löscher

Thamdrup

et al. 2019

Preprint

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13Microbial induced corrosion of steel structures, used for transport or storage of fuels, 14 chemical weapons or waste radionuclides, is an environmental and economic threat. 15In non-sulfidic environments, the exact role of methanogens in steel corrosion is 16 poorly understood. From the non-sulfidic, methanogenic sediments of the Baltic Sea 17 corrosive communities were enriched using exclusively Fe 0 as electron donor and 18 CO 2 as electron acceptor. Methane and acetate production were persistent for three 19 years of successive transfers. Methanosarcina and Clostridium were attached to the 20 Fe 0 , and dominated metagenome libraries. Since prior reports indicated 21Methanosarcina were merely commensals, consuming the acetate produced by 22 acetogens, we investigated whether these methanogens were capable of Fe 0 corrosion 23 without bacterial partners (inhibited by an antibiotic cocktail). Unassisted, 24 methanogens corroded Fe 0 to Fe 2+ at similar rates to the mixed community. 25 Surprisingly, in the absence of competitive bacteria, Baltic-Methanosarcina produced 26 six times more methane than they did in the mixed community. This signifies that 27Baltic-Methanosarcina achieved better corrosion alone, exclusive of an operative 28 bacterial partner. Our results also show that together with acetogens, Methanosarcina 29 interact competitively to retrieve electrons from Fe 0 rather than as commensals as 30 previously assumed. 31 32

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Discerning different and opposite effects of hydrogenase on the corrosion of mild steel in the presence of phosphate species

Cited by 9 publications

References 59 publications

Baltic Sea methanogens compete with acetogens for electrons from metallic iron

Baltic Sea methanogens compete with acetogens for electrons from metallic iron

A Win–Loss Interaction on Fe0 Between Methanogens and Acetogens From a Climate Lake

BalticMethanosarcinaandClostridiumcompete for electrons from metallic iron

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