Discordant Uptake of Leg Thrombi Versus Pulmonary Emboli on 68Ga-FAPI-46 PET/CT

Cho, Sang-Geon; Park, Chan; Kim, Jahae; Kim, Kang Bin; Kim, Hyung Yoon

doi:10.1097/rlu.0000000000004891

Cited by 3 publications

(2 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…68 Ga-FAP inhibitor-46 is a radiolabeled probe for the in vivo detection of FAP [7, 8, 34], and its positron emission tomography can be used to detect DVT but not pulmonary emboli in patients with venous thromboembolism [35]. Besides, our previous studies showed that DVT and pulmonary thromboembolism are composed of heterogeneous components of fresh and organizing thrombi [2, 4].…”

Section: Discussionmentioning

confidence: 99%

Expression of fibroblast activation protein-α in human deep venous thrombus

Oguri,

Gi,

Nakamura

et al. 2024

Preprint

View full text Add to dashboard Cite

BackgroundFibroblast activation protein-α (FAP), a type-II transmembrane serine protease, is expressed during wound healing, in cancer-associated fibroblasts, and in chronic fibrosing diseases. However, its expression in deep vein thrombus (DVT) remains unclear. Therefore, in this study, we investigated FAP expression and localization in DVT.MethodsFirst, we pathologically accessed aspirated thrombi of patients with DVT (n=14), classifying thrombotic areas as follows; fresh, cellular lysis, endothelialization, fibroblastic reaction. Endothelialization and fibroblastic reaction were defined as organizing reactions. We immunohistochemically examined FAP-expressed areas and expressed cells. Second, we analyzed FAP expression in cultured dermal fibroblasts.ResultsAll the aspirated thrombi showed a mixture of at least three of the four thrombotic areas. Specifically, 83 % of aspirated thrombi showed fresh and organizing reactions within each thrombus. Immunohistochemical expression of FAP was restricted in organizing area. Further, FAP expression in the thrombi was mainly found in vimentin-positive or α-smooth muscle actin-positive fibroblasts in double immunofluorescence. Some CD163-positive macrophages also showed FAP expression. FAP mRNA and protein levels significantly increased in cultured fibroblasts with low- proliferative activity under 0.1% fetal bovine serum (FBS) than that in fibroblasts under 10% FBS. Fibroblasts cultured in 10% FBS showed a significant decrease in FAP mRNA levels following supplementation with hemin, but not with thrombin.ConclusionsThe heterogeneous composition of the venous thrombi suggests the existence of a multistep thrombus formation process in human DVT. Further, fibroblasts or myofibroblasts may express FAP in organizing process in DVT. FAP expression may increase in fibroblasts with low-proliferative activity.

show abstract

Section: Discussionmentioning

confidence: 99%

Expression of fibroblast activation protein-α in human deep venous thrombus

Oguri,

Gi,

Nakamura

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…This may be due to the abnormal activity of fibroblasts in thrombosis and vascular occlusion 5,6 . In terms of cardiovascular disease, FAP is expressed in activated fibroblasts rather than quiescent fibroblasts 7–9 . This leads to a very low background signal in healthy tissue but intense activity in areas of activated fibroblasts with FAP expression.…”

mentioning

confidence: 99%

18F-FAPI-42 PET/CT Findings in a Patient With Left Ventricular Mural Thrombus

Wang,

Cheng,

Cui

et al. 2024

Clin Nucl Med

View full text Add to dashboard Cite

18F-FAPI-42 PET/CT is a novel imaging tool targeting fibroblast activation protein (FAP). We describe the 18F-FAPI-42 PET/CT findings of a left ventricular mural thrombus in a 50-year-old man who had chest tightness. The 18F-FAPI-42 PET/CT showed annular uptake at the apex of the left ventricle, but there was no uptake of 18F-FDG. This case showed that abnormal 18F-FAPI-42 uptake in the heart may be associated with mural thrombus and should be evaluated clinically.

show abstract