Discovering conserved insect microRNAs from expressed sequence tags

Jia, Qidong; Lin, Kejian; Liang, Jingdong; Yu, Lun; Li, Fēi

doi:10.1016/j.jinsphys.2010.07.007

Cited by 24 publications

(19 citation statements)

References 37 publications

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“…This was consistent with previous reports that conserved miRNAs were generally more highly expressed than newly emergent miRNAs (Ason et al 2006). We used the mature sequences of these five miRNAs to identify their homologous in 10 available Lepidopteran genomes with previously reported methods (Wang et al 2005;Jia et al 2010). When an unrestricted cutoff was set as ≤3 mismatches, homologous sequences of Csu-novel-80 could be found in all ten lepidopterans ( Supplemental Fig.…”

Section: Modulating Ecdysone Biosynthesis Requires Varied Mirnas At Dsupporting

confidence: 82%

“…Homologous sequences of the three Chilo miRNAs Csu-novel-89, Csu-novel-80, and Csu-novel-257 were found in this insect. We used a computational pipeline to predict whether the homologous sequences in these lepidopteran insects were from actual miRNA precursors (Wang et al 2005;Jia et al 2010). The results indicated that only Mse-miR-89 in Manduca sexta was predicted to be a true miRNA (Supplemental Fig.…”

Section: Modulating Ecdysone Biosynthesis Requires Varied Mirnas At Dmentioning

confidence: 99%

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Multiple miRNAs jointly regulate the biosynthesis of ecdysteroid in the holometabolous insects, Chilo suppressalis

Sun

Xiao

et al. 2017

RNA

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The accurate rise and fall of active hormones is important for insect development. The ecdysteroids must be cleared in a timely manner. However, the mechanism of suppressing the ecdysteroid biosynthesis at the right time remains unclear. Here, we sequenced a small RNA library of Chilo suppressalis and identified 300 miRNAs in this notorious rice insect pest. Microarray analysis yielded 54 differentially expressed miRNAs during metamorphosis development. Target prediction and in vitro dualluciferase assays confirmed that seven miRNAs (two conserved and five novel miRNAs) jointly targeted three Halloween genes in the ecdysteroid biosynthesis pathway. Overexpression of these seven miRNAs reduced the titer of 20-hydroxyecdysone (20E), induced mortality, and retarded development, which could be rescued by treatment with 20E. Comparative analysis indicated that the miRNA regulation of metamorphosis development is a conserved process but that the miRNAs involved are highly divergent. In all, we present evidence that both conserved and lineage-specific miRNAs have crucial roles in regulating development in insects by controlling ecdysteroid biosynthesis, which is important for ensuring developmental convergence and evolutionary diversity.

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Section: Modulating Ecdysone Biosynthesis Requires Varied Mirnas At Dsupporting

confidence: 82%

Section: Modulating Ecdysone Biosynthesis Requires Varied Mirnas At Dmentioning

confidence: 99%

Multiple miRNAs jointly regulate the biosynthesis of ecdysteroid in the holometabolous insects, Chilo suppressalis

Sun

Xiao

et al. 2017

RNA

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“…C2MC is one of the largest miRNA clusters in mice and rats [26]. Additionally, a number of miRNAs homologous to C2MC miRNAs were also found in chicken and insects [20,21]. As stated above, mouse C2MC is a recently acquired miRNA cluster derived through retrotransposon-mediated gene duplication [26,49], which contains at least 71 closely-related miRNA genes [48].…”

Section: Discussionmentioning

confidence: 90%

“…The insertions of the resultant plasmids pmiR-466a and pmiR466g were confirmed by DNA sequencing. Plasmid pmiR-574 was created as described previously [20].…”

Section: Plasmidsmentioning

confidence: 99%

Sfmbt2 10th intron-hosted miR-466(a/e)-3p are important epigenetic regulators of Nfat5 signaling, osmoregulation and urine concentration in mice

Luo

Liu

et al. 2014

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanism

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Sfmbt2-hosted miR-466a-3p and its close relatives are often among the most significantly up-regulated or down-regulated miRNAs in responses to numerous deleterious environmental stimuli. The exact roles of these miRNAs in cellular stress responses, however, are not clear. Here we showed that many Sfmbt2-hosted miRNAs were highly hypertonic stress responsive in vitro and in vivo. In renal medulla, water deprivation induced alterations in the expression of miR-466(a/b/c/e/p)-3p in a pattern similar to that of miR-200b-3p, a known regulator of osmoresponsive transcription factor Nfat5. Remarkably, exposure of mIMCD3 cells to an arginine vasopressin analog time-dependently down-regulated the expression of miR-466(a/b/c/e/p)-3p and miR-200b-3p, which provides a novel regulatory mechanism for these osmoresponsive miRNAs. In cultured mIMCD3 cells we further demonstrated that miR-466a-3p and miR-466g were capable of targeting Nfat5 by interacting with its 3'UTR. In transgenic mice overexpressing miR-466a-3p, significant down-regulation of Nfat5 and many other osmoregulation-related genes was observed in both the renal cortex and medulla. Moreover, sustained transgenic over-expression of miR-466a-3p was found to be associated with polydipsia, polyuria and disturbed ion homeostasis and kidney morphology. Since the mature sequence of miR-466a-3p is completely equivalent to that of miR-466e-3p and that the seed sequence of miR-466a-3p is completely equivalent to that of miR-297(a/b/c)-3p, miR-466d-3p, miR-467g and miR-669d-3p, and that miR-466a-3p differs from miR-466(b/c/p)-3p only in a 5' nucleotide, we propose that miR-466a-3p and many of its close relatives are important epigenetic regulators of renal Nfat5 signaling, osmoregulation and urine concentration in mice.

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“…miRNAs are evolutionally conservative, non immunogenic RNAs with an approximate length of 22 nucleotides (nt) containing a seed region (positions 2-8nt from the 5'-end of miRNA) [5]; (2) Big mighty. miRNAs play vital roles in development, cell differentiation, apoptosis, innate immunity, molecular metabolism based on post-transcriptionally and dynamically negative regulation [2,6,7,8]; (3) High efficiency.…”

Section: Introductionmentioning

confidence: 99%

miRNAs: Biogenesis, Origin and Evolution, Functions on Virus-Host Interaction

Yang

Gao

Shi

et al. 2013

Cell Physiol Biochem

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MicroRNAs (miRNAs) are small endogenous non-coding functional RNAs. They can play vital roles in post-transcriptional regulating mRNAs transcripts in nearly all biological processes. More and more reports on miRNAs come from different species (animal, plant, bacteria, virus) in the researches in development, immunity, apoptosis, tumor, virus-host interaction. These recent findings provide new insights into the roles of miRNAs as well as their function. This review outlines the ever-deepening understanding of miRNAs (biogenesis, origin, evolution), and discusses functions from host and viral miRNAs in the context of virus-host interaction.

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Discovering conserved insect microRNAs from expressed sequence tags

Cited by 24 publications

References 37 publications

Multiple miRNAs jointly regulate the biosynthesis of ecdysteroid in the holometabolous insects, Chilo suppressalis

Multiple miRNAs jointly regulate the biosynthesis of ecdysteroid in the holometabolous insects, Chilo suppressalis

Sfmbt2 10th intron-hosted miR-466(a/e)-3p are important epigenetic regulators of Nfat5 signaling, osmoregulation and urine concentration in mice

miRNAs: Biogenesis, Origin and Evolution, Functions on Virus-Host Interaction

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