2020
DOI: 10.1021/acs.orglett.0c02656
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Discovery and Biosynthesis of Clostyrylpyrones from the Obligate Anaerobe Clostridium roseum

Abstract: Anaerobic bacteria are a promising new source for natural product discovery. Examination of extracts from the obligate anaerobe Clostridium roseum led to discovery of a new family of natural products, the clostyrylpyrones. The polyketide synthasebased biosynthetic mechanism of clostyrylpyrones is further proposed based on bioinformatic, gene knockout, biochemical analysis and heterologous expression studies.

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Cited by 10 publications
(6 citation statements)
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“…7 ). Clostyrylpyrones are a class of clostridial secondary metabolites recently discovered in C. roseum , where their biosynthesis has been mapped to the csp locus, and in particular to the CspD protein 29 . Although there are no C. roseum strains in the collection, BCGs undergo high rates of horizontal gene transfer 30 .…”
Section: Resultsmentioning
confidence: 99%
“…7 ). Clostyrylpyrones are a class of clostridial secondary metabolites recently discovered in C. roseum , where their biosynthesis has been mapped to the csp locus, and in particular to the CspD protein 29 . Although there are no C. roseum strains in the collection, BCGs undergo high rates of horizontal gene transfer 30 .…”
Section: Resultsmentioning
confidence: 99%
“…Respective genes for biosynthesis were identified as cspA–E in C. roseum DSM 6424, and no homologous gene clusters were identified from public databases [27]. The same chromosomal arrangement was found in C.…”
Section: Full-textmentioning
confidence: 99%
“…The eponymous pink colour of C. roseum is caused by clostyrylpyrones A–D, a novel polyketide family [27]. Respective genes for biosynthesis were identified as cspA–E in C.…”
Section: Full-textmentioning
confidence: 99%
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“…A novel family of polyketides, including clostyrylpyrone A 29 , have been isolated from the obligate anaerobe Clostridium roseum . 25 A combination of genome mining, targeted gene deletions and heterologous expression have identified a modular PKS with a trans -acting acyltransferase responsible for the biosynthesis of the clostyrylpyrones. The substrate binding, reactivity and reaction sequence of TamI, a multi-functional P450 monooxygenase from Streptomyces species 307-9, involved in the biosynthesis of tirandamycin B 30 , have been reported.…”
mentioning
confidence: 99%