Discovery and Characterization of 1-Aminocyclopropane-1-carboxylic Acid Synthase of Bacterial Origin

Xu, Zhengren; Pan, Guohui; Zhou, Hao; Shen, Ben

doi:10.1021/jacs.8b11463

Cited by 29 publications

(35 citation statements)

References 28 publications

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“…Phylogenetic analysis suggested that Mur24 occupies a separate clade, and hence might harbor a distinct activity, when compared to plant ACC synthases or the recently characterized bacterial ACC synthase, GnmY ( Supplementary Fig. 9 ) 22 . In contrast to mur23 , genes homologous to mur24 were identified within the 2 ( cpz13 ) and 3 ( lipJ ) gene clusters, suggesting that this enzymatic step is shared in these pathways 12 , 23 .…”

Section: Resultsmentioning

confidence: 99%

Pyridoxal-5′-phosphate-dependent alkyl transfer in nucleoside antibiotic biosynthesis

et al. 2020

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Several nucleoside antibiotics are structurally characterized by a 5′′-amino-5′′-deoxyribose (ADR) appended via a glycosidic bond to a high-carbon sugar nucleoside, (5′ S ,6′ S )-5′- C -glycyluridine (GlyU). GlyU is further modified with an N -alkylamine linker, the biosynthetic origins of which have yet to be established. By using a combination of feeding experiments with isotopically labeled precursors and characterization of recombinant proteins from multiple pathways, the biosynthetic mechanism for N -alkylamine installation for ADR-GlyU-containing nucleoside antibiotics has been uncovered. The data reveal S -adenosyl- l -methionine (AdoMet) as the direct precursor of the N -alkylamine, but unlike conventional AdoMet- or decarboxylated AdoMet-dependent alkyltransferases, the reaction is catalyzed by a pyridoxal-5′-phophosate (PLP)-dependent aminobutyryltransferase (ABTase) using a stepwise γ-replacement mechanism that couples γ-elimination of AdoMet with aza-γ-addition onto the disaccharide alkyl acceptor. In addition to utilizing a conceptually different strategy for AdoMet-dependent alkylation, the newly discovered ABTases require a phosphorylated disaccharide alkyl acceptor, revealing a cryptic intermediate in the biosynthetic pathway.

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Section: Resultsmentioning

confidence: 99%

Pyridoxal-5′-phosphate-dependent alkyl transfer in nucleoside antibiotic biosynthesis

et al. 2020

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“…In another study, inoculation of soybean plants with Pseudomonas was shown to increase fresh weight and stem height under water deficit stress, with parallel increases in concentrations of Chl., SA and ABA, as compared to control plants [179]. Furthermore, application of H. seropedicae and A. brasilense in wheat cultivars led to maintenance of relative water contents, improved membrane stability as well as increased drought tolerance [180] associated with multiple mechanisms, including activation of antioxidant systems and osmolyte accumulation [181], as well as ACC-deaminase and hormonal activity [182]. It has also been stated that the useful effects of PGPB on plants such as barley (i.e., increased stress tolerance) are mediated by the accumulation of proline and several compatible solutes (osmolytes) [183].…”

Section: The Role Of Plant Growth-promoting Bacteria In Drought Stress Tolerancementioning

confidence: 91%

The Role of Plant Growth-Promoting Bacteria in Alleviating the Adverse Effects of Drought on Plants

Abdelaal

AlKahtani

Attia

et al. 2021

Biology

175

100

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Plant growth-promoting bacteria play an essential role in enhancing the physical, chemical and biological characters of soils by facilitating nutrient uptake and water flow, especially under abiotic stress conditions, which are major constrains to agricultural development and production. Drought is one of the most harmful abiotic stress and perhaps the most severe problem facing agricultural sustainability, leading to a severe shortage in crop productivity. Drought affects plant growth by causing hormonal and membrane stability perturbations, nutrient imbalance and physiological disorders. Furthermore, drought causes a remarkable decrease in leaf numbers, relative water content, sugar yield, root yield, chlorophyll a and b and ascorbic acid concentrations. However, the concentrations of total phenolic compounds, electrolyte leakage, lipid peroxidation, amounts of proline, and reactive oxygen species are considerably increased because of drought stress. This negative impact of drought can be eliminated by using plant growth-promoting bacteria (PGPB). Under drought conditions, application of PGPB can improve plant growth by adjusting hormonal balance, maintaining nutrient status and producing plant growth regulators. This role of PGPB positively affects physiological and biochemical characteristics, resulting in increased leaf numbers, sugar yield, relative water content, amounts of photosynthetic pigments and ascorbic acid. Conversely, lipid peroxidation, electrolyte leakage and amounts of proline, total phenolic compounds and reactive oxygen species are decreased under drought in the presence of PGPB. The current review gives an overview on the impact of drought on plants and the pivotal role of PGPB in mitigating the negative effects of drought by enhancing antioxidant defense systems and increasing plant growth and yield to improve sustainable agriculture.

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“…Potentially, the Fusarium ACC synthases may be active only in a complex with other proteins and could play a role in the synthesis of secondary metabolites containing ACC or ACC-like substances. Recently, a first bacterial ACC synthase was described (Xu et al, 2018), which is involved in synthesis of guangnanmycin. Coronatine, a well-known Pseudomonas metabolite interfering with defense signaling (Geng et al, 2014), contains coronamic acid, which has strong structural similarity to ACC and induces the synthesis of ethylene from methionine (Kenyon and Turner, 1992) by activating JA signaling (Uppalapati et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Biochemical Characterization of the Fusarium graminearum Candidate ACC-Deaminases and Virulence Testing of Knockout Mutant Strains

Svoboda

Parich²,

Güldener

et al. 2019

Front. Plant Sci.

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Fusarium graminearum is a plant pathogenic fungus which is able to infect wheat and other economically important cereal crop species. The role of ethylene in the interaction with host plants is unclear and controversial. We have analyzed the inventory of genes with a putative function in ethylene production or degradation of the ethylene precursor 1-aminocyclopropane carboxylic acid (ACC). F. graminearum, in contrast to other species, does not contain a candidate gene encoding ethylene-forming enzyme. Three genes with similarity to ACC synthases exist; heterologous expression of these did not reveal enzymatic activity. The F. graminearum genome contains in addition two ACC deaminase candidate genes. We have expressed both genes in E. coli and characterized the enzymatic properties of the affinity-purified products. One of the proteins had indeed ACC deaminase activity, with kinetic properties similar to ethylene-stress reducing enzymes of plant growth promoting bacteria. The other candidate was inactive with ACC but turned out to be a d-cysteine desulfhydrase. Since it had been reported that ethylene insensitivity in transgenic wheat increased Fusarium resistance and reduced the content of the mycotoxin deoxynivalenol (DON) in infected wheat, we generated single and double knockout mutants of both genes in the F. graminearum strain PH-1. No statistically significant effect of the gene disruptions on fungal spread or mycotoxin content was detected, indicating that the ability of the fungus to manipulate the production of the gaseous plant hormones ethylene and H2S is dispensable for full virulence.

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Discovery and Characterization of 1-Aminocyclopropane-1-carboxylic Acid Synthase of Bacterial Origin

Cited by 29 publications

References 28 publications

Pyridoxal-5′-phosphate-dependent alkyl transfer in nucleoside antibiotic biosynthesis

Pyridoxal-5′-phosphate-dependent alkyl transfer in nucleoside antibiotic biosynthesis

The Role of Plant Growth-Promoting Bacteria in Alleviating the Adverse Effects of Drought on Plants

Biochemical Characterization of the Fusarium graminearum Candidate ACC-Deaminases and Virulence Testing of Knockout Mutant Strains

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