Discovery and characterization of tyrosinases from sea anemone pedal disc

Wang, Lulu; Teng, Luyao; Zhang, Xiaokang; Liu, Xiaohua; Lyu, Qianqian; Yang, Yan; Liu, Weizhi

doi:10.1080/01694243.2020.1731271

Cited by 4 publications

(3 citation statements)

References 38 publications

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“…For example, by performing a homology search against the mussel foot transcriptome using tyrosinase sequences from a variety of species, Guerette et al (2013) identified several tyrosinase candidates that could be involved in the conversion of tyrosine to DOPA in the mussel P. viridis. Tyrosinases have since been detected in transcriptomes and proteomes of other mussels (Qin et al, 2016), tubeworms (Buffet et al, 2018), and sea anemones (Wang et al, 2020; see also Table 3). Using a similar approach, Wang, Suhre & Scheibel (2019) retrieved the sequence of a polyphenol oxidase in MytiBase (Venier et al, 2009).…”

Section: Challenges: How Can Bioadhesion Research Continue To Benefit From 'Omics?mentioning

confidence: 99%

Omics‐based molecular analyses of adhesion by aquatic invertebrates

et al. 2021

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Many aquatic invertebrates are associated with surfaces, using adhesives to attach to the substratum for locomotion, prey capture, reproduction, building or defence. Their intriguing and sophisticated biological glues have been the focus of study for decades. In all but a couple of specific taxa, however, the precise mechanisms by which the bioadhesives stick to surfaces underwater and (in many cases) harden have proved to be elusive. Since the bulk components are known to be based on proteins in most organisms, the opportunities provided by advancing 'omics technologies have revolutionised bioadhesion research. Time-consuming isolation and analysis of single molecules has been either replaced or augmented by the generation of massive data sets that describe the organism's translated genes and proteins. While these new approaches have provided resources and opportunities that have enabled physiological insights and taxonomic comparisons that were not previously possible, they do not provide the complete picture and continued multi-disciplinarity is essential. This review covers the various ways in which 'omics have contributed to our understanding of adhesion by aquatic invertebrates, with new data to illustrate key points. The associated challenges are highlighted and priorities are suggested for future research.

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Section: Challenges: How Can Bioadhesion Research Continue To Benefit From 'Omics?mentioning

confidence: 99%

Omics‐based molecular analyses of adhesion by aquatic invertebrates

et al. 2021

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“…Sea anemones are typical sessile marine invertebrates that live in intertidal zones. Sea anemones secrete adhesives from their pedal discs to firmly adhere to the substratum [ 20 ]. In this study, we applied combined omics to efficiently discover and produce an antioxidant protein.…”

Section: Introductionmentioning

confidence: 99%

Exploration of sea anemone-inspired high-performance biomaterials with enhanced antioxidant activity

Wang

Zhang

et al. 2022

Bioactive Materials

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Antioxidant biomaterials have attracted much attention in various biomedical fields because of their effective inhibition and elimination of reactive oxygen species (ROS) in pathological tissues. However, the difficulty in ensuring biocompatibility, biodegradability and bioavailability of antioxidant materials has limited their further development. Novel bioavailable antioxidant materials that are derived from natural resources are urgently needed. Here, an integrated multi-omics method was applied to fabricate antioxidant biomaterials. A key cysteine-rich thrombospondin-1 type I repeat-like (TSRL) protein was efficiently discovered from among 1262 adhesive components and then used to create a recombinant protein with a yield of 500 mg L −1 . The biocompatible TSRL protein was able to self-assemble into either a water-resistant coating through Ca 2+ -mediated coordination or redox-responsive hydrogels with tunable physical properties. The TSRL-based hydrogels showed stronger 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rates than glutathione (GSH) and ascorbic acid (Aa) and protected cells against external oxidative stress significantly more effectively. When topically applied to mice skin, TSRL alleviated epidermal hyperplasia and suppressed the degradation of collagen and elastic fibers caused by ultraviolet radiation B (UVB) irradiation, confirming that it enhanced antioxidant activity in vivo . This is the first study to successfully characterize natural antioxidant biomaterials created from marine invertebrate adhesives, and the findings indicate the excellent prospects of these biomaterials for great applications in tissue regeneration and cosmeceuticals.

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“…The cylindrical bodies are attached to the shells of other animals and to rocks on the seafloor by powerful suction cups at the base. This bonding mechanism is related to the strong adhesive secreted by the anemone's pedal discs [62] In a study, Wang et al [63] (Figure 8B) applied multi-omics techniques and genetic engineering to effectively discover and transcribe an antioxidant protein. A key cysteine-rich antioxidant protein (thrombospondin-1 type I repeat-like, TSRL) was filtrated from 1262 adhesive components.…”

Section: Bioinspired Hydrogels With Other Responsive Characteristicsmentioning

confidence: 99%

Recent Advances in Bioinspired Hydrogels with Environment‐Responsive Characteristics for Biomedical Applications

Ran

Wang

et al. 2022

Macromolecular Bioscience

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The development of hydrogel‐integrated soft materials via the incorporation of therapeutic medicines into biocompatible hydrogels, serving as host, will significantly contribute to advances in medical diagnosis and treatment. Furthermore, intelligent hydrogels having the ability to respond to local environmental conditions offer a promising approach for the development of novel solutions in the biomedical field. Bioinspired intelligent hydrogels are now becoming a potentially powerful biomaterial class for tissue engineering, drug delivery, and medical device. Recent advances include bioinspired intelligent hydrogels that possess unique mechanical and optical properties as a result of their nature‐inspired complex‐structured design. This review highlights the latest advances in intelligent bionic hydrogels, as well as strategies targeting smart response of their characteristics across multiple dimensions (such as temperature, light, pH, among others). Finally, the potential development and prospective application of mimicking the natural intelligence of multifunctional medical hydrogels are also discussed.

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Discovery and characterization of tyrosinases from sea anemone pedal disc

Cited by 4 publications

References 38 publications

Omics‐based molecular analyses of adhesion by aquatic invertebrates

Omics‐based molecular analyses of adhesion by aquatic invertebrates

Exploration of sea anemone-inspired high-performance biomaterials with enhanced antioxidant activity

Recent Advances in Bioinspired Hydrogels with Environment‐Responsive Characteristics for Biomedical Applications

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