Discovery of a functional Mycosphaerella teleomorph in the presumed asexual barley pathogen Septoria passerinii

Ware, S.B.; Verstappen, E.C.P.; Breeden, Jill; Cavaletto, Jessica R.; Goodwin, Stephen B.; Waalwijk, C.; Crous, Pedro W.; Kema, G.H.J.

doi:10.1016/j.fgb.2006.12.005

Cited by 22 publications

(15 citation statements)

References 39 publications

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“…We estimated that the genome size for the three M. graminicola isolates is 31-40 Mb, which fits with data from several other fungi that were derived from detailed PFGE studies and genome sequencing projects (Beadle et al 2003, Galagan et al 2003, Dean et al 2005. The difference in genome size between IPO323 and IPO94269 is approximately 17% but does not preclude the generation of viable sexual offspring (Kema et al 2000, Kema et al 2002, Ware et al 2007. This suggests a substantial redundancy in the genome of IPO323 not affecting development, sexual reproduction and pathogenicity and that possibly can be attributed to the larger number and size of chromosomes in this isolate, which might originate from duplication and translocation during meiosis (Wittenberg et al 2007).…”

Section: Discussionmentioning

confidence: 97%

“…The Dutch strains are mating type testers and are the parents of the first M. graminicola mapping population (Kema et al 2002, Goodwin et al 2007. The genome of IPO323 currently is sequenced at US Department of Energy Joint Genome Institute, and IPO94241 was included as a reference from earlier electrophoretic karyotypic analysis (McDonald and Martinez 1991).…”

Section: Methodsmentioning

confidence: 99%

“…In this study we combined PFGE and the germ tube burst method (GTBM; Shirane et al 1988, Taga et al 1994 to analyze the chromosomal complement of M. graminicola isolates that were used in genetic mapping and genomic sequencing programs (Kema et al 2002, Goodwin et al 2007. We conclude that M. graminicola has an unusually wide chromosome size range and the largest chromosome number observed in ascomycetes (see reviews by Beadle et al 2003, Walz 2004.…”

Section: Introductionmentioning

confidence: 99%

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Electrophoretic and cytological karyotyping of the foliar wheat pathogenMycosphaerella graminicolareveals many chromosomes with a large size range

Mehrabi

Taga

Kema³

2007

Mycologia

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The karyotypes of three isolates of Mycosphaerella graminicola, the septoria tritici blotch pathogen of wheat, were analyzed with both pulsed field gel electrophoresis (PFGE) and the cytological technique called germ tube burst method (GTBM). These analyses revealed a chromosome length polymorphism among these isolates. The estimated genome size was 31-40 Mb depending on the isolates, indicating 17-22% redundancy in the genome of the standard strain IPO323 because such differences do not affect development, pathogenicity and sexual reproduction of the other isolates. The chromosome numbers in the three isolates were 18-20 and the chromosome size was 0.3-6 Mb. These data show that M. graminicola has the highest chromosome number and the smallest autosomes (A chromosomes) in filamentous ascomycetes. Our data also confirmed a large ($6 Mb) chromosome that was assembled recently in the IPO323 genome sequence. GTBM analyses revealed the mitotic metaphase chromosomes, enabling chromosome quantification, which was fully congruent with the PFGE analyses. These data will be instrumental in the final assembly of the M. graminicola genome.

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Section: Discussionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Electrophoretic and cytological karyotyping of the foliar wheat pathogenMycosphaerella graminicolareveals many chromosomes with a large size range

Mehrabi

Taga

Kema³

2007

Mycologia

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“…The identification and experimental crossing of heterothallic fungi has resulted in the discovery of sexual stages in species that are pathogenic to humans (Hull et al, 2000;Magee and Magee, 2000) and that contaminate food and invade crops (Houbraken et al, 2008;Ware et al, 2007). Aspergillus parasiticus belongs to section Flavi (Peterson, 2008) and many of the species within the section produce sclerotia.…”

Section: Ascocarp Formationmentioning

confidence: 99%

Sexual reproduction and recombination in the aflatoxin-producing fungus Aspergillus parasiticus

Horn

Ramírez-Prado

Carbone

2009

Fungal Genetics and Biology

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“…However, one explanation is that some of the supposedly asexual species may have a cryptic sexual cycle (8). Another possibility is that sexual reproduction is a rare event that is hard to detect (74) and thus remains to be described. Nevertheless, the activation of a sex-ual cycle in A. chrysogenum would increase options for alternative strain improvement strategies.…”

Section: Vol 74 2008 Mating Type Locus Of Acremonium Chrysogenum 6011mentioning

confidence: 99%

Asexual Cephalosporin C Producer Acremonium chrysogenum Carries a Functional Mating Type Locus

Pöggeler

Hoff

Kück

2008

Appl Environ Microbiol

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Acremonium chrysogenum, the fungal producer of the pharmaceutically relevant ␤-lactam antibiotic cephalosporin C, is classified as asexual because no direct observation of mating or meiosis has yet been reported. To assess the potential of A. chrysogenum for sexual reproduction, we screened an expressed sequence tag library from A. chrysogenum for the expression of mating type (MAT) genes, which are the key regulators of sexual reproduction. We identified two putative mating type genes that are homologues of the ␣-box domain gene, MAT1-1-1 and MAT1-1-2, encoding an HPG domain protein defined by the presence of the three invariant amino acids histidine, proline, and glycine. In addition, cDNAs encoding a putative pheromone receptor and pheromone-processing enzymes, as well as components of a pheromone response pathway, were found. Moreover, the entire A. chrysogenum MAT1-1 (AcMAT1-1) gene and regions flanking the MAT region were obtained from a genomic cosmid library, and sequence analysis revealed that in addition to AcMAT1-1-1 and AcMAT1-1-2, the AcMAT1-1 locus comprises a third mating type gene, AcMAT1-1-3, encoding a high-mobility-group domain protein. The ␣-box domain sequence of AcMAT1-1-1 was used to determine the phylogenetic relationships of A. chrysogenum to other ascomycetes. To determine the functionality of the AcMAT1-1 locus, the entire MAT locus was transferred into a MAT deletion strain of the heterothallic ascomycete Podospora anserina (the Pa⌬MAT strain). After fertilization with a P. anserina MAT1-2 (MAT ؉ ) strain, the corresponding transformants developed fruiting bodies with mature ascospores. Thus, the results of our functional analysis of the AcMAT1-1 locus provide strong evidence to hypothesize a sexual cycle in A. chrysogenum.

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Discovery of a functional Mycosphaerella teleomorph in the presumed asexual barley pathogen Septoria passerinii

Cited by 22 publications

References 39 publications

Electrophoretic and cytological karyotyping of the foliar wheat pathogenMycosphaerella graminicolareveals many chromosomes with a large size range

Electrophoretic and cytological karyotyping of the foliar wheat pathogenMycosphaerella graminicolareveals many chromosomes with a large size range

Sexual reproduction and recombination in the aflatoxin-producing fungus Aspergillus parasiticus

Asexual Cephalosporin C Producer Acremonium chrysogenum Carries a Functional Mating Type Locus

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