Discovery of loci determining pre-harvest sprouting and dormancy in wheat and barley applying segregation and association mapping

Lohwasser, U.; Arif, Mian Abdur Rehman; Börner, Andreas

doi:10.1007/s10535-013-0332-2

Cited by 25 publications

(20 citation statements)

References 60 publications

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“…As with the red seed coat genes of the group three chromosomes, one may hypothesize the overlap as being related in function. Lohwasser et al (2013) also reported dormancy QTL QD20.ipk-1D on chromosome 1D just 2.2 cM from QPhs.spa-1D. The minor effect QPhs.spa-1A1 QTL at cfa2135 coincides with a QTL reported by Knox et al (2012) and Jaiswal et al (2012), and is near to QTL reported in many other studies (Glein et al 2006;Munkvold et al 2009;Mohan et al 2009;Singh et al 2010;Rehman et al 2012;Singh et al 2014).…”

Section: Discussionsupporting

confidence: 72%

“…Chromosomes of homoeologous group 4 are considered to have an effective role in pre-harvest sprouting resistance in wheat through dormancy control [Kulwal et al 2010 (review);Lohwasser et al 2013]. Noda et al (2002) investigated another ABA responsive gene located on the long arm of chromosome 4A.…”

Section: Discussionmentioning

confidence: 99%

“…Noda et al (2002) investigated another ABA responsive gene located on the long arm of chromosome 4A. QPhs.spa-4A, located in interval barc170-gwm397-wmc617a on 4A, is in a region associated with dormancy and preharvest sprouting resistance in numerous other QTL studies of hexaploid wheat (Flintham et al 2002;Torada et al 2005;Mares et al 2005;Tan et al 2006;Ogbonnaya et al 2008;Imtiaz et al 2008;Munkvold et al 2009;Rasul et al 2009;Kulwal et al 2010;Singh et al 2010Singh et al , 2012Lohwasser et al 2013). The locus was also identified in tetraploid ) and diploid (Nakamura et al 2007) wheat populations, and in an association mapping study (Kulwal et al 2012).…”

Section: Discussionmentioning

confidence: 99%

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Maximizing the identification of QTL for pre-harvest sprouting resistance using seed dormancy measures in a white-grained hexaploid wheat population

et al. 2015

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Pre-harvest sprouting in spring wheat causes significant financial loss to growers throughout the world and sprouting damage can be reduced by growing resistant genotypes. Several genetic factors, especially those related to seed dormancy, are involved in the control of pre-harvest sprouting resistance. The objective of this study was to identify quantitative trait loci (QTL) influencing pre-harvest sprouting resistance from multiple measures of dormancy at multiple germination intervals on seed harvested across multiple environments. A doubled haploid mapping population of 91 individuals derived from a cross of two Canadian white-seeded spring wheat genotypes, SC8021-V2 (pre-harvest sprouting resistant) and AC Karma (moderately susceptible to pre-harvest sprouting) was used for QTL mapping. Daily germination counts were analysed using germination index, germination resistance and percent germination at intervals of 3, 5, 7, 10, 14 and 21 days from spike samples collected from six field and one greenhouse environments in Saskatchewan, Canada. Continuous frequency distributions at certain measure-durations indicated genetic complexity of dormancy segregation in the SC8021-V2/AC Karma cross. Composite interval mapping detected significant (p B 0.05) QTL associated with resistance to pre-harvest sprouting on all 21 wheat chromosomes. Of the 26 total QTL, six were novel and the rest were detected either at the same marker or overlapping a marker interval reported in other studies. QTL expressed consistently for germination index, germination resistance and percent germination at different germination durations on chromosomes 2B, 4A, 5D and 6D. QTL identified on homoeologous chromosomes 4A, 4B and 4D with chromosome specific molecular variants of SSR marker wmc617 suggest a conserved region for controlling dormancy on group four. The majority of QTL mapped in regions known to contain factors affecting different components of pre-harvest sprouting resistance like seed dormancy, seed coat colour, ABA responsiveness and alpha-amylase activity. This study demonstrated that using multiple measures of seed dormancy Electronic supplementary material The online version of this article (doi:10.1007/s10681-015-1460-x) contains supplementary material, which is available to authorized users. Euphytica (2015) 205:287-309 DOI 10.1007/s10681-015-1460 at multiple intervals of germination enhanced identification of QTL affecting dormancy in white-seeded hexaploid wheat.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Maximizing the identification of QTL for pre-harvest sprouting resistance using seed dormancy measures in a white-grained hexaploid wheat population

et al. 2015

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“…By linkage mapping analysis, four were validated as major dormancy loci on 4H, 5H, and 7H (Han et al, 1996). The QTLs in the centromere (SD1) and in the telomeric region of the long arm (SD2) of 5H are considered as the most important dormancy QTLs (Lohwasser, Arif, & Börner, 2013;Romagosa, Han, Clancy, & Ullrich, 1999;Sato, Matsumoto, Ooe, & Takeda, 2009 3) is directly located at this telomeric region in barley. MKK3 is a key signalling module for plant growth and development processes (Xu & Zhang, 2015) and was identified as a strong candidate for barley seed dormancy (Nakamura et al, 2016) and for PHS in an orthologue region of the wheat chromosome 4A (Torada et al, 2016).…”

Section: Marker-trait Associationsmentioning

confidence: 99%

Novel loci and a role for nitric oxide for seed dormancy and preharvest sprouting in barley

Nagel

Alqudah

Bailly

et al. 2019

Plant Cell & Environment

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Barley is used for food and feed, and brewing. Nondormant seeds are required for malting, but the lack of dormancy can lead to preharvest sprouting (PHS), which is also undesired. Here, we report several new loci that modulate barley seed dormancy and PHS. Using genome‐wide association mapping of 184 spring barley genotypes, we identified four new, highly significant associations on chromosomes 1H, 3H, and 5H previously not associated with barley seed dormancy or PHS. A total of 71 responsible genes were found mostly related to flowering time and hormone signalling. A homolog of the well‐known Arabidopsis Delay of Germination 1 (DOG1) gene was annotated on the barley chromosome 3H. Unexpectedly, DOG1 appears to play only a minor role in barley seed dormancy. However, the gibberellin oxidase gene HvGA20ox1 contributed to dormancy alleviation, and another seven important loci changed significantly during after‐ripening. Furthermore, nitric oxide release correlated negatively with dormancy and shared 27 associations. Origin and growth environment affected seed dormancy and PHS more than did agronomic traits. Days to anthesis and maturity were shorter when seeds were produced under drier conditions, seeds were less dormant, and PHS increased, with a heritability of 0.57–0.80. The results are expected to be useful for crop improvement.

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“…Therefore, grain dormancy is deterrent to preharvest sprouting (PHS) and the level of grain dormancy can be used for the assessment of PHS tolerance (Rehman-Arif et al 2012;Lohwasser et al 2013). Grain colour of wheat, influence the flour brightness by contaminating the red pigment in the milling process (Warner et al 2000;Groos et al 2002;Himi et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Molecular survey of Tamyb10-1 genes and their association with grain colour and germinability in Chinese wheat and Aegilops tauschii

Dong

Chen

et al. 2015

J Genet

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To investigate allelic variation of Myb10-1 genes in Chinese wheat and to examine its association with germination level in wheat, a total of 582 Chinese bread wheat cultivars and 110 Aegilops tauschii accessions were used to identify allelic variations of three Myb10-1 genes. Identification results indicated that there is a novel Tamyb10-B1 allele, designated Tamyb10-B1c, in the five Chinese landraces. The Tamyb10-B1c possibly has a large deletion including Tamyb10-B1 gene. There are three novel Tamyb10-D1 alleles (Aetmyb10-D1c, Aetmyb10-D1d and Aetmyb10-D1e) that were discovered in Aegilops tauschii. Of them, Aetmyb10-D1c allele possessed a 104-bp deletion and this resulted in a frame shift in the open reading frame of the Aetmyb10-D1 gene. AETMYB10-D1d and AETMYB10-D1e proteins possessed three and two different amino acids when compared with TAMYB10-D1b protein, respectively. Association of Tamyb10-1 allelic variation with grain germination level indicated that all five allelic combinations with red grains showed a significantly higher GP (germination percentage) and GI (germination index) values than those of white-grained Tamyb10-A1a/Tamyb10-B1a/Tamyb10-D1a genotype after storing it for one year. Moreover, the Tamyb10-A1b/Tamyb10-B1c/Tamyb10-D1b genotype possesses the significantly highest GP and GI among the six different Tamyb10-1 combinations. This study could provide useful information for wheat breeding programme in terms of grain colour and germination level.

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Discovery of loci determining pre-harvest sprouting and dormancy in wheat and barley applying segregation and association mapping

Cited by 25 publications

References 60 publications

Maximizing the identification of QTL for pre-harvest sprouting resistance using seed dormancy measures in a white-grained hexaploid wheat population

Maximizing the identification of QTL for pre-harvest sprouting resistance using seed dormancy measures in a white-grained hexaploid wheat population

Novel loci and a role for nitric oxide for seed dormancy and preharvest sprouting in barley

Molecular survey of Tamyb10-1 genes and their association with grain colour and germinability in Chinese wheat and Aegilops tauschii

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