2016 International Congress of Entomology 2016
DOI: 10.1603/ice.2016.111203
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Discovery of midgut genes for the RNA interference control of corn rootworm

Abstract: RNA interference (RNAi) is a promising new technology for corn rootworm control. This paper presents the discovery of new gene targets -dvssj1 and dvssj2, in western corn rootworm (WCR). Dvssj1 and dvssj2 are orthologs of the Drosophila genes snakeskin (ssk) and mesh, respectively. These genes encode membrane proteins associated with smooth septate junctions (SSJ) which are required for intestinal barrier function. Based on bioinformatics analysis, dvssj1 appears to be an arthropod-specific gene. Diet based in… Show more

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Cited by 10 publications
(23 citation statements)
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“…RNAi has become well established as an effective technology to control WCR with several lethal targets documented. 17,23,24,26,27 The first commercial maize product expressing insecticidal dsRNA for WCR management will have three modes of action, dvSnf7 dsRNA and two Bt proteins Cry3Bb1 and Cry34/35Ab1. 27,70 The The root damage rating data estimated on 0-1.0 NIS scale was converted into binomial categorical data and the pass frequency (≤0.50 root rating) for each construct was calculated using a generalized linear model (JMP ® Pro 12.2.0).…”
Section: Discussionmentioning
confidence: 99%
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“…RNAi has become well established as an effective technology to control WCR with several lethal targets documented. 17,23,24,26,27 The first commercial maize product expressing insecticidal dsRNA for WCR management will have three modes of action, dvSnf7 dsRNA and two Bt proteins Cry3Bb1 and Cry34/35Ab1. 27,70 The The root damage rating data estimated on 0-1.0 NIS scale was converted into binomial categorical data and the pass frequency (≤0.50 root rating) for each construct was calculated using a generalized linear model (JMP ® Pro 12.2.0).…”
Section: Discussionmentioning
confidence: 99%
“…One should also note that known potent RNAi targets such as v-ATPase A are enriched in the midgut epithelium. 26,75 The loss of Sec23 in Drosophila perturbs epidermal adherents and septate junctions. 73 As described by Hu et al, 26 the depletion of adherent junction transcripts, mesh, and snakeskin, via dsRNA feeding in WCR also leads to strong lethal phenotypes.…”
Section: Discussionmentioning
confidence: 99%
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“…For aquatic larvae, dissolving dsRNA in solution and bathing the larvae within it, is the most common method of effecting gene silencing via eRNAi (Figueira-Mansur et al, 2013;Singh et al, 2013;Whyard et al, 2015;Bona et al, 2016). dsRNA can be delivered to non-aquatic larvae: (1) topically via droplet feeding (Toprak et al, 2013), (2) by inducing the larvae to feed upon dsRNA-expressing transgenic plants (Xiong et al, 2013;Mamta et al, 2015;Tian et al, 2015;Hu et al, 2016), (3) by feeding larvae dsRNA-expressing transgenic bacteria (Zhu et al, 2011;Yang & Han, 2014;Li et al, 2015c), and (4) by feeding larvae naked dsRNA overlaid onto an artificial diet (Asokan et al, 2014;Yang & Han, 2014;Hu et al, 2016). Non-aquatic larvae, or those that develop in relatively anoxic conditions, can also be bathed in dsRNA solution, but the timing of exposure is critical to avoid drowning (Whyard et al, 2009).…”
Section: Ernai Delivery Methods: Larvaementioning
confidence: 99%