Discovery of Neuropeptides in the Ganglia of the Pacific Abalone Haliotis discus hannai Using Long-Read Transcriptome Analysis

Oh, Young Dae; Choi, Mi‐Jin; Cho, Youn Su; Jeong, Tae Hyug; Kim, Jong‐Myoung; Lim, Han Kyu

doi:10.2983/035.039.0110

Cited by 4 publications

(3 citation statements)

References 92 publications

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“…The Pacific abalone Haliotis discus hannai is one of the most commercially valuable shellfish species in northeast Asia [ 1 ]. Various methods have been implemented to improve its productivity and foster its beneficial traits; these methods include selective breeding, hybridization, and transcriptomic approaches [ 2 , 3 , 4 , 5 , 6 ]. Researchers have also screened for faster growth in Pacific abalone to reduce the time necessary for the shellfish to reach a marketable size.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a C-Type Lectin Domain-Containing Protein with Antibacterial Activity from Pacific Abalone (Haliotis discus hannai)

Choi

Kim

Park

et al. 2022

IJMS

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Genes that influence the growth of Pacific abalone (Haliotis discus hannai) may improve the productivity of the aquaculture industry. Previous research demonstrated that the differential expression of a gene encoding a C-type lectin domain-containing protein (CTLD) was associated with a faster growth in Pacific abalone. We analyzed this gene and identified an open reading frame that consisted of 145 amino acids. The sequence showed a significant homology to other genes that encode CTLDs in the genus Haliotis. Expression profiling analysis at different developmental stages and from various tissues showed that the gene was first expressed at approximately 50 days after fertilization (shell length of 2.47 ± 0.13 mm). In adult Pacific abalone, the gene was strongly expressed in the epipodium, gill, and mantle. Recombinant Pacific abalone CTLD purified from Escherichia coli exhibited antimicrobial activity against several Gram-positive bacteria (Bacillus subtilis, Streptococcus iniae, and Lactococcus garvieae) and Gram-negative bacteria (Vibrio alginolyticus and Vibrio harveyi). We also performed bacterial agglutination assays in the presence of Ca2+, as well as bacterial binding assays in the presence of the detergent dodecyl maltoside. Incubation with E. coli and B. subtilis cells suggested that the CTLD stimulated Ca2+-dependent bacterial agglutination. Our results suggest that this novel Pacific abalone CTLD is important for the pathogen recognition in the gastropod host defense mechanism.

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Section: Introductionmentioning

confidence: 99%

“…Researchers have also screened for faster growth in Pacific abalone to reduce the time necessary for the shellfish to reach a marketable size. In a previous study, we screened differentially expressed genes associated with faster growth in Pacific abalone and identified three genes involved in immune-related responses [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of a C-Type Lectin Domain-Containing Protein with Antibacterial Activity from Pacific Abalone (Haliotis discus hannai)

Choi

Kim

Park

et al. 2022

IJMS

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“…Nonetheless, the biomolecular coverage of the commercially important Pacific abalone (H. discus hannai) remains limited [15]. Genomic and transcriptomic analyses have been used to identify meaningful markers for abalone growth [19,20,22,[28][29][30]. A transcriptomic study of sexual maturation in H. discus hannai has uncovered certain pathways related to maturation, with lipid pathways being crucial in this process [19].…”

Section: Introductionmentioning

confidence: 99%

Lipid Profiling of Pacific Abalone (Haliotis discus hannai) at Different Developmental Stages Using Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry

Lee

Joo

Park³

et al. 2022

Journal of Analytical Methods in Chemistry

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Pacific abalone (Haliotis discus hannai) is a commercially important mollusk; therefore, improvement of its growth performance and quality has been emphasized. During embryonic development, abalones undergo a series of distinct larval stages, including swimming veliger larvae, juveniles, and mature individuals, and their biomolecular composition varies depending on the developmental stage. Therefore, in the present study, we performed untargeted lipid profiling of abalone tissues at different developmental stages as well as the hemolymph of mature female and male abalones using ultrahigh-performance liquid chromatography-tandem mass spectrometry. These profiles can provide meaningful information to understand compositional changes in lipids through abalone metamorphosis and development. A total of 132 lipids belonging to 15 classes were identified from abalone tissues at different developmental stages. Moreover, 21 lipids belonging to 8 classes were identified from the hemolymph of mature abalones. All data were processed following strict criteria to provide accurate information. Triglycerides and phosphatidylcholines were the major lipid components identified in both tissues and hemolymph, accounting for, respectively, 27% and 15% of all lipids in tissues and, respectively, 24% and 38% of all lipids in the hemolymph. Of note, lysophosphatidylcholine was only detected in the tissues of mature abalones, paving the way for further analyses of abalone lipids based on developmental stages. The present findings offer novel insights into the lipidome of abalone tissues and hemolymph at different developmental stages, building a foundation for improving the efficiency and quality of abalone aquaculture.

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Molecular characterization of four genes encoding abalone insulin-related peptides and their roles in regulation of hemolymph glucose in the Pacific abalone Haliotis discus hannai

Choi,

Oh,

Chung

et al. 2023

Aquaculture

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Discovery of Neuropeptides in the Ganglia of the Pacific Abalone Haliotis discus hannai Using Long-Read Transcriptome Analysis

Cited by 4 publications

References 92 publications

Characterization of a C-Type Lectin Domain-Containing Protein with Antibacterial Activity from Pacific Abalone (Haliotis discus hannai)

Characterization of a C-Type Lectin Domain-Containing Protein with Antibacterial Activity from Pacific Abalone (Haliotis discus hannai)

Lipid Profiling of Pacific Abalone (Haliotis discus hannai) at Different Developmental Stages Using Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry

Molecular characterization of four genes encoding abalone insulin-related peptides and their roles in regulation of hemolymph glucose in the Pacific abalone Haliotis discus hannai

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