Characterization of a C-Type Lectin Domain-Containing Protein with Antibacterial Activity from Pacific Abalone (Haliotis discus hannai)

Abstract

Genes that influence the growth of Pacific abalone (Haliotis discus hannai) may improve the productivity of the aquaculture industry. Previous research demonstrated that the differential expression of a gene encoding a C-type lectin domain-containing protein (CTLD) was associated with a faster growth in Pacific abalone. We analyzed this gene and identified an open reading frame that consisted of 145 amino acids. The sequence showed a significant homology to other genes that encode CTLDs in the genus Haliotis. Expression profiling analysis at different developmental stages and from various tissues showed that the gene was first expressed at approximately 50 days after fertilization (shell length of 2.47 ± 0.13 mm). In adult Pacific abalone, the gene was strongly expressed in the epipodium, gill, and mantle. Recombinant Pacific abalone CTLD purified from Escherichia coli exhibited antimicrobial activity against several Gram-positive bacteria (Bacillus subtilis, Streptococcus iniae, and Lactococcus garvieae) and Gram-negative bacteria (Vibrio alginolyticus and Vibrio harveyi). We also performed bacterial agglutination assays in the presence of Ca2+, as well as bacterial binding assays in the presence of the detergent dodecyl maltoside. Incubation with E. coli and B. subtilis cells suggested that the CTLD stimulated Ca2+-dependent bacterial agglutination. Our results suggest that this novel Pacific abalone CTLD is important for the pathogen recognition in the gastropod host defense mechanism.

Transcriptomic response of Mytilus coruscus mantle to acute sea water acidification and shell damage

“…This indicates different functions of different perlucin transcripts under different stressors. Perlucin has been reported to be involved in the innate immune defense in shrimp and abalones (Bi et al, 2020;Choi et al, 2022). Considering the multiple functions of perlucin in both biomineralization and innate immunity, we speculate that some perlucins may participate in innate immune defense, while others are involved in shell formation in Mytilus, and different responses of different perlucin transcripts to acute acidification and shell damage may reflect the complex roles of this molecule.…”

Systemic Lectin-Glycan Interaction of Pathogenic Enteric Bacteria in the Gastrointestinal Tract

“…These are evolutionarily conserved in pathogenic bacteria, viruses, fungi, and protists [6]. PAMPs interact at the molecular level with lectin proteins, such as C-type lectins, galectins, and Siglecs, which are distributed among plants, invertebrates, and vertebrates [1,8].…”