Discovery of Pantoea rodasii Strain ND03 that Produces N-(3-Oxo-hexanoyl)-L-homoserine Lactone

Abstract

Proteobacteria use quorum sensing to regulate target gene expression in response to population density. Quorum sensing (QS) is achieved via so-called signalling molecules and the best-studied QS signalling system uses N-acyl homoserine lactones (AHLs). This study aimed to identify and characterize the production of AHLs by a bacterium ND03 isolated from a Malaysian tropical rainforest waterfall. Molecular identification showed that ND03 is a Pantoea sp. closely related to Pantoea rodasii. We used Chromobacterium violaceum CV026, an AHL biosensor for preliminary AHL production screening and then used high resolution triple quadrupole liquid chromatography-mass spectrometry, to confirm that P. rodasii strain ND03 produced N-(3-oxo-hexanoyl)-l-homoserine lactone (3-oxo-C6-HSL). To the best of our knowledge, this is the first report for such a discovery in P. rodasii strain ND03.

Pseudomonas cremoricolorata Strain ND07 Produces N-acyl Homoserine Lactones as Quorum Sensing Molecules

“…The temperatures and pH of the samples were recorded. Water samples were kept in an icebox and brought to the laboratory for further analysis [ 12 ].…”

Occurrence of Biofilm Formation in Serratia fonticola and Pantoea sp. Isolates among Urinary Catheterized Patients

“…It was pointed out that Pantoea members had been used to synthesize QS which regulated many phenotypes, such as the production of virulence factor, the aggregation of cells and biofilm formation [30] Pantoea sp. showed QS activity by increasing cell density-dependent exopolysaccharide substance (EPS) synthesis [17,31]. Exopolysaccharide (EPS) was a major component of biofilm bacterial matrix and a powerful virulence factor which protected the bacterial cell from antibiotics action and hosted immune defense [32].…”

Understanding the Quorum-Sensing Bacterium Pantoea stewartii Strain M009 with Whole-Genome Sequencing Analysis

“…The communication occurs when small diffusible molecules in a given bacterial population synchronize and stimulate a series of gene expressions that could drive different responses, such as the production of virulence factors ( 2 , 3 ). Since QS plays a vital role for bacteria, it is therefore important to study the freshwater-inhabiting bacteria that exhibit QS properties, because freshwater can serve as a reservoir for microorganisms ( 3 , 4 ). Pantoea stewartii has long been known for causing Stewart’s wilt in sweet corn, and epidemics in the 1990s led to significant economic losses for the corn seed industry ( 5 ).…”