Discriminating nevus and melanoma on paraffin-embedded skin biopsies using FTIR microspectroscopy

Tfayli, Ali; Piot, Olivier; Durlach, A.; Bernard, Philippe; Manfait, Michel

doi:10.1016/j.bbagen.2005.04.020

Cited by 106 publications

(104 citation statements)

References 22 publications

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“…Spectral range for the acquired spectra was from 620 to 1810 cm 21 , acquisition time of each spectrum is two times 10 s, and the confocal pinhole was adjusted at 150 lm.…”

Section: Instrumentation Raman Spectrometermentioning

confidence: 99%

“…The phenylalanine also contributes to the 1175 and 1339 cm 21 vibrations. 19,25,[38][39][40][41][42][43][44] The presence of tyrosine (Tyr) is highlighted by the 643 cm 21 band (ring breathing) and the Raman doublet at 829 and 851 cm 21 , this doublet is due to Fermi resonance between the ring breathing vibration, and the overtone of the nonplanar ring vibration at 413 cm 21 . The intensity ratio I 829 /I 851 is related to the state of the phenolic hydroxyl group.…”

Section: Proteins Of the Episkin Modelmentioning

confidence: 99%

“…Here, it is difficult to have such information because the contribution of cysteines, major amino acids of the epidermis, presenting a band at 642 cm 21 assigned to the CÀ ÀS stretching and hiding the tyrosine vibration. 8,[38][39][40][41][42] The tryptophane (Trp) vibrational bands are present at 758, 1340, and 1550 cm 21 . The 883 cm 21 peak could also be assigned to the tryptophane; this peak is shifted in frequency (from 873 to 883 cm 21 ) when the residue is in aqueous liquid.…”

Section: Proteins Of the Episkin Modelmentioning

confidence: 99%

“…The 883 cm 21 peak could also be assigned to the tryptophane; this peak is shifted in frequency (from 873 to 883 cm 21 ) when the residue is in aqueous liquid. The lack of the vibration peak at 1363 cm 21 shows that the tryptophane ring is exposed and therefore accessible to molecules of water. 8,25,38,40,41 At 1602 cm 21 , a low-intensity vibration highlights the glutamine and glutamic acid presence.…”

Section: Proteins Of the Episkin Modelmentioning

confidence: 99%

“…15 The potential of Raman spectroscopy to discriminate pathologic from healthy skin structures and to classify different types and grades of cutaneous lesions has been demonstrated by several studies. [16][17][18][19][20][21][22][23][24] Other research works studied the molecular composition of the skin, 25,26 followed the effects induced by different types of drugs, 25,27 and established in depth concentration profiles (starting from the surface and going deeper into the skin) of different molecules (water and endogenous molecules). [27][28][29][30][31][32][33][34] The follow-up, in real-time analysis, of the diffusion of the exogenous products through skin layers has also been performed.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of reconstructed skin model by Raman microspectroscopy: Comparison with excised human skin

et al. 2007

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Human skin is directly exposed to different exogenous agents. Many research works have studied the diffusion, interactions, absorption mechanisms, and/or toxicity of these agents toward different cutaneous structures. With the use of living animals for such tests being more and more rejected; and the number of human volunteers being limited; different types of skin models are used. In the last few years, reconstructed epidermis from cell cultures has been frequently employed, and recent changes in the European chemical policy have approved and encouraged the use of these reconstructed models for skin‐related research works and assessments. Among the techniques used actually to study the skin, Raman microspectroscopy is a rising and powerful nondestructive technique that detects characteristic molecular vibrations. In this study, we created a spectral database to index the vibration peaks and bands of a well‐known reconstructed epidermis model, the Episkin®. The comparison with a native epidermis signal enabled us to put in evidence several spectral differences associated with molecular and structural differences between the skin and the reconstructed model, both maintained in living conditions. In addition to that, we have showed the feasibility of tracking the penetration of a pharmaceutical molecule through the Episkin model. © 2007 Wiley Periodicals, Inc. Biopolymers 87: 261–274, 2007.This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

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“…Spectral range for the acquired spectra was from 620 to 1810 cm 21 , acquisition time of each spectrum is two times 10 s, and the confocal pinhole was adjusted at 150 lm.…”

Section: Instrumentation Raman Spectrometermentioning

confidence: 99%

Section: Proteins Of the Episkin Modelmentioning

confidence: 99%

Section: Proteins Of the Episkin Modelmentioning

confidence: 99%

Section: Proteins Of the Episkin Modelmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of reconstructed skin model by Raman microspectroscopy: Comparison with excised human skin

et al. 2007

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Introduction to Spectral Imaging, and Applications to Diagnosis of Lymph Nodes

Romeo¹,

Dukor²,

Diem³

2001

Handbook of Vibrational Spectroscopy

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This chapter introduces the field of vibrational spectroscopic imaging and discusses the advances made to date in terms of biomedical diagnosis. The reader is introduced to the spectral signatures of typical cellular components, and the need for an objective method for cancer diagnosis is discussed. Besides an overview of typical instrumentation and components, this chapter provides a working application, namely the diagnosis of metastatic cancer in lymph nodes. Through this application methods of sample preparation, data acquisition and data preprocessing and analysis are discussed.

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Infrared and Raman Spectroscopic Imaging

Steiner

2012

Biomedical Imaging

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Discriminating nevus and melanoma on paraffin-embedded skin biopsies using FTIR microspectroscopy

Cited by 106 publications

References 22 publications

Molecular characterization of reconstructed skin model by Raman microspectroscopy: Comparison with excised human skin

Molecular characterization of reconstructed skin model by Raman microspectroscopy: Comparison with excised human skin

Introduction to Spectral Imaging, and Applications to Diagnosis of Lymph Nodes

Infrared and Raman Spectroscopic Imaging

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