Discrimination between some species of Zygophyllaceae based on SEM and Start Codon Targeted (Scot) markers

Moawed, Mohamed M.; Ibrahim, Sherif

doi:10.5455/egyjebb.20160622105237

Cited by 3 publications

(5 citation statements)

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“…Hereditary characterizations of the halophyte Suaeda maritima in Thailand given by SCoT profiles ( Rittirongsakul et al, 2020 ), and cultivar characters based on hereditary solidness with SCoT molecular marker in modern new Polish lines of Chenopodium quinoa Willd ( Lema-Rumińska et al, 2018 ). Comparable discoveries were gotten with few molecular examinations of Zygophyllaceae ( Hammad and Qari, 2010 , Moawed and Ibrahim, 2016 , Sheahan and Chase, 2000 ). Comparable come about were gotten in which SCoT primers appeared particular unmistakable bands permitting the separation of appointed summer squash landraces ( Xanthopoulou et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

“…Molecular markers have been utilized within the development of molecular relation maps, hereditary differences investigations, cultivar identification, and molecular marker-assisted breeding ( Kalia et al, 2011 ). Molecular markers, such as inter simple sequence repeat (ISSR) and start codon targeted (SCoT) have been connected for genetic diversity investigations for a few species of Zygophyllaceae ( Morsy and El Sherbeny, 2015 , Moawed and Ibrahim, 2016 , Abd-Elgawad and Alotaibi, 2019 ). These molecular marker strategies are broadly utilized for hereditary differences examination, as they are basic and can deliver a gigantic number of DNA markers in any test.…”

Section: Introductionmentioning

confidence: 99%

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ISSR and SCoT for evaluation of hereditary differences of 29 wild plants in Al Jubail Saudi Arabian

Alotaibi

Abd-Elgawad

2022

Saudi Journal of Biological Sciences

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

ISSR and SCoT for evaluation of hereditary differences of 29 wild plants in Al Jubail Saudi Arabian

Alotaibi

Abd-Elgawad

2022

Saudi Journal of Biological Sciences

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“…For DNA, the DNA was extracted from stem sample of Ephedra Plant by using a DNAeasy Plant Mini Kit (QIAGEN, Santa Clarita, CA) according to (Moawed and Ibrahim, 2016). Also, the genomic DNA was isolated from harvested mycelia of A. flavus (25 mg) with or without the treatment with (ESE) following instruction of the protocol for DNA Tissue purification mini kit by grinding the liquid N2 frozen tissue in a mortar.…”

Section: Extraction Of Nucleic Acidsmentioning

confidence: 99%

The inhibitory Effect of different Ephedra plant extracts on the Aspergillus flavus growth and Aflatoxin B1 gene Expression

2022

JJBS

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This study was conducted to investigate the effects of Ephedra sinica extracts (ESE) on Aspergillus flavus growth, AFB 1 production and O-methyltransferase A (Omt-A) gene expression. The following various extracts such as Ethanol (EtOH), Ethanol: water (EtOH: H 2 O 1:1, v/v), Methanol (MeOH), and Methanol: water (MeOH: H 2 O 1:1, v/v) at concentration of 1.0, 2.0, 3.0, 4.0, and 5.0% have been used. AFB 1 production and gene expression were measured using HPLC and real time PCR, respectively. Our findings demonstrated that all ESE has significant inhibition (73.3-94.6 %) for Aspergillus flavus growth based on the dose. Production of AFB 1 was markedly decreased or totally inhibited within range of 10-99.3% depends on extract type. Supported the effect of ESE at concentrations of 1-5% were suppressed significantly (P<0.05) on aflatoxin (Omt-A) gene expression. The nucleotides sequence of Ephedra plant was submitted in GeneBank under accession number KY310531.1. These outcomes might be used to control the growth of toxigenic fungi and to inhibit the following contamination of feed, food, and agricultural supplies by carcinogenic aflatoxins.

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“…Start codon targeted (SCoT) technique conventional according to Collard and Mackill (2009) depends on short, conserved region in genes of plants surrounding the ATG translation start codon. This technique is used for amplifying multiple regions of the genome which could be used for refined between different plant species (Moawed and Ibrahim 2016, Satya et al 2015, Zhang et al 2015, Shahlaei et al 2014, Amirmoradi et al 2012, Xiong et al 2011. SCoT polymorphism was achieving reputation for its advantage over other dominant DNA marker techniques (Mulpuri et al 2013, Gorji et al 2011.…”

Section: Introductionmentioning

confidence: 99%

“…SCoT polymorphism was achieving reputation for its advantage over other dominant DNA marker techniques (Mulpuri et al 2013, Gorji et al 2011. In Egypt, Moawed and Ibrahim (2016) characterized and distinguished a number of 17 taxa of 13 species and five genera belonging to family Zygophyllaceae collected from Saudi Arabia and Egypt by SEM and SCoT technique.…”

Section: Introductionmentioning

confidence: 99%

Genetic Variation of Three BBTV-Infected Banana Cultivars Based on SCoT DNA Marker Technique

El-Masry,

Ibrahim,

Abdel-Razek

et al. 2024

Journal of Agricultural Chemistry and Biotechnology

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Several banana cultivars are grown in all types of farming systems, which Egyptians use in a mature way due to their nutritional value, and banana bunchy top virus (BBTV) contributive pathogen of Banana Bunchy Top Disease (BBTD) remains to be the furthermost devastating pathogen, which is threatening production in major banana growing areas. The current study was planned to determine the genetic variations of some banana cultivars using some molecular tools. Genetic variations of three BBTV-infected banana cultivars (Edward Cavendish, Maghrabi and Williams) collected from Qalyubia Governorate compared to healthy banana ones determined by using two molecular techniques, i.e., start codon targeted (SCoT) and/or SDS-PAGE. SCoT technique was also used to determine DNA fingerprinting of Maghrabi plant cultivar grown under different climate conditions. Results showed that, quantum of genetic variation in genome of similar variety whatsoever healthy or infected with BBTV was highest probable in Maghrabi cultivar (6-9%) followed by Williams cultivar (3-6%) and the lowest was Edward Cavendish cultivar (3-4%). It was proved that identities percentage between samples of two regions of Qalyubia Governorate exceeds 70% and that they both found in same cluster, while identities percentage between samples of both Qena and Aswan increased more than 80%. Results of SDS-PAGE technique showed twelve protein bands (five monomorphic and seven polymorphic bands) and there aren't any protein bands found that might be reflected as a distinctive marker protein. It was also noted that both healthy and BBTV-infected samples from the same region were found together in a separate cluster.

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Discrimination between some species of Zygophyllaceae based on SEM and Start Codon Targeted (Scot) markers

Cited by 3 publications

References 0 publications

ISSR and SCoT for evaluation of hereditary differences of 29 wild plants in Al Jubail Saudi Arabian

ISSR and SCoT for evaluation of hereditary differences of 29 wild plants in Al Jubail Saudi Arabian

The inhibitory Effect of different Ephedra plant extracts on the Aspergillus flavus growth and Aflatoxin B1 gene Expression

Genetic Variation of Three BBTV-Infected Banana Cultivars Based on SCoT DNA Marker Technique

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