Discrimination of living or dead cells of diatom based on the stained images - Its method and significance.

Kosugi, Masato

doi:10.4116/jaqua.24.139

Cited by 10 publications

(2 citation statements)

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“…To assess the diatoms in the samples (MS1-MS5), the formalin fixed samples were rinsed in pure water in the laboratory, pre-treated with haematoxylin-eosin stain (Kosugi 1985;Sawai 2001) to distinguish between living and dead cells, and mounted on slides with Pleurax medium (Mount Media; Wako, Osaka, Japan). The sample of the N. subamphioxoides type material was cleaned by H 2 O 2 treatment.…”

Section: Methodsmentioning

confidence: 99%

Nitzschia taikiensis sp. nov. (Bacillariophyta) – a new brackish diatom from the Toberi River marsh in the eastern Hokkaido, Japan

Chiba

Nishimura

Horie

et al. 2020

Biodiversity Research and Conservation

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Nitzschia taikiensis sp. nov. is a brackish diatom species found in the Toberi River marsh, eastern Hokkaido, Japan. This species has characteristics similar to Nitzschia subamphioxoides Hustedt, which was originally described by Hustedt in 1959. In the present study, we conducted a comparative morphological analysis of N. taikiensis and N. subamphioxoides. The obtained results showed that they are different species. Sampling sites of N. taikiensis were located in salt marsh environments with very low salinity (1-5‰), acidic pH (5.2-5.9) and high mud content (95.0-97.5%). Identification of this species was relatively easy based on LM and SEM image analysis of its frustule features, such as the external form and stria density. This species has not been previously reported in Japan, which could be attributed to the reduction or loss of Japanese freshwater and salt marsh environments in the coastal areas, resulting from urban and industrial development.

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Section: Methodsmentioning

confidence: 99%

Nitzschia taikiensis sp. nov. (Bacillariophyta) – a new brackish diatom from the Toberi River marsh in the eastern Hokkaido, Japan

Chiba

Nishimura

Horie

et al. 2020

Biodiversity Research and Conservation

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show abstract

“…However, it is not suitable for the evaluation of living diatoms because the simultaneous observation of nuclear fluorescence and the fine structures of valves is impossible. Other staining methods such as Acetocarmine staining (Mayama & Kobayasi 1982), Hematoxylin and Eosin staining (Kosugi 1985), and Hematoxylin staining (Gotoh 1988) enable the observation of fine valve structures and therefore are suitable for both living and dead diatoms. Of these methods, the simplest and most practical is the Hematoxylin staining method developed by Gotoh (1988).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of three preparation methods for living diatoms at a sandy river-mouth tidal flat: conventional acid-cleaning, nuclear staining, and sieving

Yamamoto¹,

Ohtsuka²

2020

Fottea

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Three preparation methods for the identification of diatom flora at a river mouth tidal flat were evaluated in terms of species richness, diversity, and composition using a conventional acid-cleaning method, a nuclear staining method, and a newly introduced sieving method. Twenty-five diatom samples were collected from Fujimae Tidal Flat sediments, Nagoya, Japan, in July 2014. Three methods respectively showed different aspects of the diatom flora. The acid-cleaning method indicated the highest species diversity, but it extracted dead diatoms with living ones indiscriminately. In comparison with the acid-cleaning method, the staining method effectively extracted diatoms with fragile and small-sized cells. The sieving method scraped the sessile diatoms selectively and supplemented the staining method in which sessile diatoms were mostly neglected. A combination of these three methods enables to describe precise diatom flora of a tidal flat.

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Distinguishing reworked diatoms derived from Neogene marine strata in modern coastal assemblages for understanding taphonomic processes and reconstructing Holocene paleoenvironments in the Tokachi coastal area, Hokkaido, Japan

Chiba

Nishimura

Yanagisawa

2021

Marine Micropaleontology

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Discrimination of living or dead cells of diatom based on the stained images - Its method and significance.

Cited by 10 publications

References 0 publications

Nitzschia taikiensis sp. nov. (Bacillariophyta) – a new brackish diatom from the Toberi River marsh in the eastern Hokkaido, Japan

Nitzschia taikiensis sp. nov. (Bacillariophyta) – a new brackish diatom from the Toberi River marsh in the eastern Hokkaido, Japan

Evaluation of three preparation methods for living diatoms at a sandy river-mouth tidal flat: conventional acid-cleaning, nuclear staining, and sieving

Distinguishing reworked diatoms derived from Neogene marine strata in modern coastal assemblages for understanding taphonomic processes and reconstructing Holocene paleoenvironments in the Tokachi coastal area, Hokkaido, Japan

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