Discrimination of major and minor streptococci incriminated in bovine mastitis by MALDI-TOF MS fingerprinting and 16S rRNA gene sequencing

Alnakip, Mohamed E. A.; Rhouma, Nasreddin R.; Abdelfatah, Eman Nabil; Quintela-Baluja, Marcos; Böhme, Karola; Fernández-No, Inmaculada C.; Bayoumi, Mohamed A.; Abdelhafez, Mostafa M.; Taboada-Rodríguez, Amaury; Calo-Mata, Pilar; Barros‐Velázquez, Jorge

doi:10.1016/j.rvsc.2020.07.027

Cited by 23 publications

(23 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To extrapolate the activity of PlySs2 and PlySs9 from the S. uberis 0140J reference strain to the same pathogen but currently occurring in infected dairy cattle, a panel of recently isolated subclinical and clinical S. uberis was composed and challenged by TRA with both endolysins at 0.5 µM during 1 h. The strains originated from various dairy farms and were freshly isolated from milk samples by the Milk Control Center of Flanders (Belgium). They were complementary verified by MALDI-TOF (data not shown) [38]. The reference strain S. uberis ATCC 27,985 was additionally included.…”

Section: Uberis Strainsmentioning

confidence: 99%

Characterization of the Bacteriophage-Derived Endolysins PlySs2 and PlySs9 with In Vitro Lytic Activity against Bovine Mastitis Streptococcus uberis

et al. 2020

View full text Add to dashboard Cite

Bovine mastitis, an infection of the cow’s mammary gland, is frequently caused by Streptococcus uberis and causes major economic losses in the dairy industry. The intramammary administration of antibiotics currently remains the predominant preventive and therapeutic measure. These antimicrobial compounds, of which some are considered critical in human health care, are frequently applied as dry therapy resulting in their consistent overuse. Therefore, the use of antibiotics in the dairy sector is being questioned. We here identified two endolysins, i.e., PlySs2 and PlySs9, respectively derived from Streptococcus suis serotype-2 and -9 prophages, with lytic activity against S. uberis in an in vitro setting. Both endolysins gave clear lysis zones in spot-on-plate assays and caused a reduction of the optical density in a turbidity reduction assay. In depth characterization identified PlySs9 as the more potent endolysin over PlySs2 with a lower MIC value and about one additional log of killing. PlySs2 and PlySs9 were challenged to a panel of subclinical and clinical S. uberis milk isolates and were both able to lyse all strains tested. Molecular dissection of these endolysins in catalytic and cell wall binding subdomains resulted in major loss of killing and binding activity, respectively. Taken together, we here propose PlySs2 and PlySs9 as candidate compounds to the current antimicrobial arsenal known against bovine mastitis-causing S. uberis as future add-on or replacement strategy to the currently used intramammary antibiotics.

show abstract

Section: Uberis Strainsmentioning

confidence: 99%

Characterization of the Bacteriophage-Derived Endolysins PlySs2 and PlySs9 with In Vitro Lytic Activity against Bovine Mastitis Streptococcus uberis

et al. 2020

View full text Add to dashboard Cite

show abstract

“…S. canis, S. equinus , and S. lutetiensis are rarely associated with bovine mastitis and were diagnosed in approximately <1% of all streptococcal infections [ 27 , 28 , 29 ]. For the streptococcal species rarely inducing bovine mastitis, reservoirs and risk factors are largely unknown.…”

Section: Reservoirs Occurrence and Frequency Of Streptococcal Mastitismentioning

confidence: 99%

“…Protein-based mass spectrometry is the optical identification of pathogen-specific peptides by their weight and correlated time of flight. A mass spectrometry study of [ 27 ] identified three biomarkers specific for bovine streptococci with peaks at m/z values of 2550, 2634, and 2650. The identification of the causative streptococcal species was possible with specific other peaks as well ( S. agalactiae : 2112, S. dysgalactiae : 5955, and S. uberis : 4452).…”

Section: Diagnosis Of Streptococcal Mastitismentioning

confidence: 99%

The Role of Streptococcus spp. in Bovine Mastitis

et al. 2021

View full text Add to dashboard Cite

The Streptococcus genus belongs to one of the major pathogen groups inducing bovine mastitis. In the dairy industry, mastitis is the most common and costly disease. It not only negatively impacts economic profit due to milk losses and therapy costs, but it is an important animal health and welfare issue as well. This review describes a classification, reservoirs, and frequencies of the most relevant Streptococcus species inducing bovine mastitis (S. agalactiae, S. dysgalactiae and S. uberis). Host and environmental factors influencing mastitis susceptibility and infection rates will be discussed, because it has been indicated that Streptococcus herd prevalence is much higher than mastitis rates. After infection, we report the sequence of cow immune reactions and differences in virulence factors of the main Streptococcus species. Different mastitis detection techniques together with possible conventional and alternative therapies are described. The standard approach treating streptococcal mastitis is the application of ß-lactam antibiotics. In streptococci, increased antimicrobial resistance rates were identified against enrofloxacin, tetracycline, and erythromycin. At the end, control and prevention measures will be considered, including vaccination, hygiene plan, and further interventions. It is the aim of this review to estimate the contribution and to provide detailed knowledge about the role of the Streptococcus genus in bovine mastitis.

show abstract

“…Alnakip et al . (2020), however, achieved differentiation between S. dysgalactiae subspecies isolated from bovine mastitis using MALDI-TOF MS fingerprinting with a detailed spectral analysis of characteristic genus and species-specific biomarkers. The three isolates identified as S. equinus / S. lutetiensis by MALDI-TOF MS had identification scores below 2.0.…”

Section: Discussionmentioning

confidence: 99%

“…(2016) also reported low discrimination of S. equinus / S. lutetiensis and S. gallolyticus using MALDI-TOF MS. On the other hand, Alnakip et al . (2020) reported successful differentiation of all streptococcal isolates at the species and subspecies level, including S. equinus and S. gallolyticus , using MALDI-TOF MS fingerprinting and Data Explorer ® analysis software (v. 4.0).…”

Section: Discussionmentioning

confidence: 99%

Identification of bovine mastitis pathogens using MALDI-TOF mass spectrometry in Brazil

Oliveira

Brito

Marval

et al. 2021

Journal of Dairy Research

View full text Add to dashboard Cite

In this Research Communication we evaluate the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify 380 bacteria isolated from cases of bovine mastitis in Brazil. MALDI-TOF MS identifications were compared to previous identifications by biochemical tests and 16S rRNA sequencing. MALDI-TOF MS achieved a typeability of 95.5%. The accuracy of MALDI-TOF MS for the identification of Staphylococcus isolates was 93.2%. The agreement between MALDI-TOF MS and biochemical identification of Streptococcus agalactiae was 96%, however, the agreement between these techniques for identifying other catalase-negative, Gram-positive cocci was lower. Agreement in identifying Gram-negative bacteria at the genus level was 90.5%. Our findings corroborate that MALDI-TOF MS is an accurate, rapid and simple technique for identifying bovine mastitis pathogens. The availability of this methodology in some research institutions would represent a significant step toward increasing the diagnosis and epidemiological studies of bovine mastitis and other animal infectious diseases in Brazil.

show abstract

Discrimination of major and minor streptococci incriminated in bovine mastitis by MALDI-TOF MS fingerprinting and 16S rRNA gene sequencing

Cited by 23 publications

References 64 publications

Characterization of the Bacteriophage-Derived Endolysins PlySs2 and PlySs9 with In Vitro Lytic Activity against Bovine Mastitis Streptococcus uberis

Characterization of the Bacteriophage-Derived Endolysins PlySs2 and PlySs9 with In Vitro Lytic Activity against Bovine Mastitis Streptococcus uberis

The Role of Streptococcus spp. in Bovine Mastitis

Identification of bovine mastitis pathogens using MALDI-TOF mass spectrometry in Brazil

Contact Info

Product

Resources

About