Disease Resistance Conferred by Constitutive Expression of a Fungal Glucose Oxidase Gene in Transgenic Tobacco Plants

Selvakumar, Palaniappan; Arvinth, S.; Maruthasal, Subbiyan; Lin, Chin-Ho

doi:10.3923/ajps.2013.128.136

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…Endogenous H 2 O 2 levels of tobacco plants ( Nicotiana tobaccum L. cv. SR1) were enhanced by constitutively expressing a GOD gene isolated from A. niger , and transgenic tobacco plants exhibited resistance to leaf spot fungal disease and bacterial wilt disease (Selvakumar et al, 2013 ). Similarly, the GOD gene from A. niger was inserted into potato plants, which resulted in leaves and tubers that produced high amounts of H 2 O 2 constitutively and acquired resistance to the bacterium Pectobacterium carotovorum sub sp.…”

Section: Applications Of God In Various Sectorsmentioning

confidence: 99%

Improvement Strategies, Cost Effective Production, and Potential Applications of Fungal Glucose Oxidase (GOD): Current Updates

et al. 2017

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Fungal glucose oxidase (GOD) is widely employed in the different sectors of food industries for use in baking products, dry egg powder, beverages, and gluconic acid production. GOD also has several other novel applications in chemical, pharmaceutical, textile, and other biotechnological industries. The electrochemical suitability of GOD catalyzed reactions has enabled its successful use in bioelectronic devices, particularly biofuel cells, and biosensors. Other crucial aspects of GOD such as improved feeding efficiency in response to GOD supplemental diet, roles in antimicrobial activities, and enhancing pathogen defense response, thereby providing induced resistance in plants have also been reported. Moreover, the medical science, another emerging branch where GOD was recently reported to induce several apoptosis characteristics as well as cellular senescence by downregulating Klotho gene expression. These widespread applications of GOD have led to increased demand for more extensive research to improve its production, characterization, and enhanced stability to enable long term usages. Currently, GOD is mainly produced and purified from Aspergillus niger and Penicillium species, but the yield is relatively low and the purification process is troublesome. It is practical to build an excellent GOD-producing strain. Therefore, the present review describes innovative methods of enhancing fungal GOD production by using genetic and non-genetic approaches in-depth along with purification techniques. The review also highlights current research progress in the cost effective production of GOD, including key advances, potential applications and limitations. Therefore, there is an extensive need to commercialize these processes by developing and optimizing novel strategies for cost effective GOD production.

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Section: Applications Of God In Various Sectorsmentioning

confidence: 99%

Improvement Strategies, Cost Effective Production, and Potential Applications of Fungal Glucose Oxidase (GOD): Current Updates

et al. 2017

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“…Такими организмами являются некоторые грибы, у которых для этого существует фермент -глюкозооксидаза и соответствующий ген для ее синтеза (ген GOX) (https://ru.wikipedia.org/wiki/). Этот ген под контролем конститутивных промоторов (CaMV S35, figwort mosaic virus 35S), включенный в геном растений, заставлял их продуцировать H 2 O 2 в количествах, значительно превышающих собственные возможности растений [Wu et al, 1995[Wu et al, , 1997Савчин и др., 2012;Selvakumar et al, 2013]. Эти авторы показали, что трансформированные растения картофеля лучше противостояли грибным и бактериальным патогенам.…”

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EFFECTS OF POTATO (SKARB VARIETY) TRANSFORMED WITH GOX GENE FROM PENICILLIUM FISTULOSUM ON THE GROWTH OF NONPATHOGENIC ESHERICHIA COLI AND PHYTOPATHOGENIC PECTOBACTERIUM CAROTOVORUM AND CLAVIBACTER MICHIGANENSIS BACTERIA

Gamburg

Быбин

Боровский

et al. 2018

Book of Proceedings of the All-Russian Scientific Conference With International Participation and Schools of Young Scientists

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Федеральное государственное бюджетное учреждение науки Сибирский институт физиологии и биохимии растений Сибирского отделения Российской академии наук, Иркутск, Россия, gamburg@sifibr.irk.ru Аннотация. Трансгенные растения картофеля c геном GOX ингибировали размножение клеток непатогенной бактерии (Esherichia coli) и фитопатогенных бактерий (Pectobacterium carotovorum и Clavibacter michiganensis). Патогенные бактерии были способны использовать вещества растения -хозяина в качестве дополнительного источника питания, что ускоряло размножение их клеток, в отличие от непатогенной бактерии, а наличие гена GOX у трансгенов препятствовало этому. C. michiganensis не передвигалась из части стебля с корнями, погруженной в бактериальную суспензию, в ту часть стебля, которая с ней не контактировала.Abstract. Transgenic potato plants with GOX gene inhibited cell propagation of the nonpathogenic E. coli and phytopathogenic P. carotovorum and C. michiganensis bacteria. Propagation of pathogenic bacteria was stimulated with some substances of the host plants, but the presence of GOX gene decreased this effect. C. michiganensis did not move from lower rooted stem part immersed in bacterium suspension into the upper part which did not contacted with it.

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Manipulation of Reactive Oxygen Species, Redox and Nitric Oxide Signaling Systems to Activate Plant Innate Immunity for Crop Disease Management

Vidhyasekaran

2020

Signaling and Communication in Plants

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Disease Resistance Conferred by Constitutive Expression of a Fungal Glucose Oxidase Gene in Transgenic Tobacco Plants

Cited by 3 publications

References 19 publications

Improvement Strategies, Cost Effective Production, and Potential Applications of Fungal Glucose Oxidase (GOD): Current Updates

Improvement Strategies, Cost Effective Production, and Potential Applications of Fungal Glucose Oxidase (GOD): Current Updates

EFFECTS OF POTATO (SKARB VARIETY) TRANSFORMED WITH GOX GENE FROM PENICILLIUM FISTULOSUM ON THE GROWTH OF NONPATHOGENIC ESHERICHIA COLI AND PHYTOPATHOGENIC PECTOBACTERIUM CAROTOVORUM AND CLAVIBACTER MICHIGANENSIS BACTERIA

Manipulation of Reactive Oxygen Species, Redox and Nitric Oxide Signaling Systems to Activate Plant Innate Immunity for Crop Disease Management

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