Disinfection of explants for saffron (Crocus sativus) tissue culture

Silva, J. A. T. da; Kulus, Dariusz; Zhang, Xinhua; Zeng, Songjun; Ma, Guohua; Piqueras, Abel

doi:10.22364/eeb.14.25

Cited by 41 publications

(30 citation statements)

References 71 publications

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“…Contamination in plant tissue cultures can be produced by various micro-arthropods (mites, trips, and their vectors), microorganisms (filamentous fungi, yeasts, bacteria), viruses, and viroids (Altan et al, 2010; Da Silva et al, 2016a; Hesami et al, 2018c). Therefore, sterilization step is of high paramount during establishing and maintaining plants in in vitro cultures.…”

Section: Introductionmentioning

confidence: 99%

Modeling and Optimizing in vitro Sterilization of Chrysanthemum via Multilayer Perceptron-Non-dominated Sorting Genetic Algorithm-II (MLP-NSGAII)

2019

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In vitro sterilization is a primary step of plant tissue culture which the ultimate results of in vitro culture are directly depended on the efficiency of the sterilization. Artificial intelligence models in a combination of optimization algorithms could be beneficial computational approaches for modeling and optimizing in vitro culture. The aim of this study was modeling and optimizing in vitro sterilization of chrysanthemum, as a case study, through Multilayer Perceptron- Non-dominated Sorting Genetic Algorithm-II (MLP-NSGAII). MLP was used for modeling two outputs including contamination frequency (CF), and explant viability (EV) based on seven variables including HgCl2, Ca(ClO)2, Nano-silver, H2O2, NaOCl, AgNO3, and immersion times. Subsequently, models were linked to NSGAII for optimizing the process, and the importance of each input was evaluated by sensitivity analysis. Results showed all of the R2 of training and testing data were over 94%. According to MLP-NSGAII, optimal CF (0%), and EV (99.98%) can be obtained from 1.62% NaOCl at 13.96 min immersion time. The results of sensitivity analysis showed that CF and EV were more sensitive to immersion time and less sensitive to AgNO3. Subsequently, the performance of predicted and optimized sterilants × immersion times combination were tested, and results indicated that the differences between the MLP predicted and validation data were negligible. Generally, MLP-NSGAII as a powerful methodology may pave the way for establishing new computational strategies in plant tissue culture.

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Section: Introductionmentioning

confidence: 99%

Modeling and Optimizing in vitro Sterilization of Chrysanthemum via Multilayer Perceptron-Non-dominated Sorting Genetic Algorithm-II (MLP-NSGAII)

2019

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“…The explant source (i.e., mother plant) and the procedure to surface disinfect explants are important aspects underlying the success of a tissue culture protocol (Leifert et al 1994;Teixeira da Silva et al 2016c). Information about the explants used for the in vitro propagation of P. santalinus, as well as surface disinfection protocols, are summarised in Table 1.…”

Section: Micropropagation Explantsmentioning

confidence: 99%

Red sandalwood (Pterocarpus santalinus L. f.): biology, importance, propagation and micropropagation

Silva

Kher

Soner³

et al. 2018

J. For. Res.

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Pterocarpus santalinus L. f. (Fabaceae; red sanders) is prized for its wood whose colour and fragrance is due to the presence of santalins that have pharmaceutical and industrial uses. Red sanders is listed as an endangered plant species on the IUCN red data list as a result of the exploitation of its wood and essential oil. This review emphasizes the pollination biology, seed germination, vegetative propagation and micropropagation of P. santalinus. Excessive use of P. santalinus has also caused the emergence of various adulterants, so accurate identification is essential. Keywords Conservation Á Fabaceae Á IUCN red data list Á Medicinal plant Á Micropropagation Á Red sanders Historical, cultural, medicinal, and economic importance of red sanders as a basis for conservation Pterocarpus santalinus L. f. (Fabaceae) is most commonly known as red sandalwood in English, but it also has other common names in several languages (Table S1). Pterocarpus is derived from the Greek words pteron (wing) and karpos (fruit), referring to the winged pod, while santalinus originates from the Latin sandal and inus (meaning similar to), i.e., a plant with characteristics similar to Indian sandalwood, Santalum album L. (Botanical Survey of India 2012). Like African or Nepalese sandalwood (Teixeira da Silva et al. 2016a) and Indian sandalwood (Teixeira da Silva et al. 2016b), P. santalinus is also prized for its hard, darkpurple, bitter heartwood (Navada and Vittal 2014). In India, the natural range of P. santalinus used to be a very restricted area of 15,540 km 2 in the southeast (Sarma 1993). Currently, P. santalinus is found exclusively in a well-defined forest tract of Andhra Pradesh in Southern India (Raju and Nagaraju 1999; Prakash et al. 2006; Balaraju et al. 2011), but is also found in the Chinese provinces of Yunnan, Guangdong and Guangxi, and on Hainan Island, where it is referred to as zitan (Kaner et al. 2013). The colour and fragrance of P. santalinus heartwood are derived from santalins while the pleasent aroma is caused by the presence of terpenoids (Kumar et al. 1974). A dye prepared from the heartwood of P. santalinus is used as a stain in light microscopy (Banerjee and Mukherjee 1981; Sen Gupta and Mukherjee 1981), as a coloring agent in pharmaceutical preparations, in food, leather and textile industries

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“…The choice of an explant and its disinfection process can affect the success of micropropagation (Teixeira da Silva et al, 2016b), including the date palm. Shoot tips and adventitious shoots in lateral buds contain more meristematic tissues than other organs, and therefore are frequently used in date palm tissue cultures (Rashid and Quraishi, 1994;Mazri and Meziani, 2015).…”

Section: Explant Selectionmentioning

confidence: 99%

Date palm ( Phoenix dactylifera L.) biotechnology: a mini-review

Khokhar¹,

Silva²

2017

bta

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Date palm (Phoenix dactylifera L.) is the source of fruit and palm oil, which is broadly used in the food industry.The regeneration of recalcitrant date palm genotypes through somatic embryogenesis or organogenesis is difficult to achieve. Micropropagation, however, provides a way to obtain a sufficient number of true-to-type elite, healthy (disease-free) and vigorous plants to satisfy local and international markets. This mini-review highlights some of the main achievements in the tissue culture of date palm. In particular, explant selection and disinfection, shoot induction, multiplication and elongation, root induction and acclimatization are highlighted. In addition to using the in vitro tissue culture as the basis for an in vitro gene banking, a mid-term low-temperature storage of germplasm is possible by careful selection of the osmotic agent. A long-term storage of date palm using cryopreservation, with or without synthetic seeds is also possible. Molecular markers, as well as sex-specific markers, have been useful to discriminate germplasms and to identify somaclonal variants derived from tissue cultures. The genetic transformation of date palm can be achieved by either particle bombardment or Agrobacterium -based protocols. Thus, biotechnology is an important element of date palm germplasm development and its sustainable improvement.

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Disinfection of explants for saffron (Crocus sativus) tissue culture

Cited by 41 publications

References 71 publications

Modeling and Optimizing in vitro Sterilization of Chrysanthemum via Multilayer Perceptron-Non-dominated Sorting Genetic Algorithm-II (MLP-NSGAII)

Modeling and Optimizing in vitro Sterilization of Chrysanthemum via Multilayer Perceptron-Non-dominated Sorting Genetic Algorithm-II (MLP-NSGAII)

Red sandalwood (Pterocarpus santalinus L. f.): biology, importance, propagation and micropropagation

Date palm ( Phoenix dactylifera L.) biotechnology: a mini-review

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