Disinfection of Seed Surfaces with Sodium Hypochlorite

Sauer, David; Burroughs, R.

doi:10.1094/phyto-76-745

Cited by 191 publications

(108 citation statements)

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“…Healthy and uniform common bean seeds of each genotype were surface-sterilized with 7% sodium hypochlorite (NaOCl) for 20 min (Sauer and Burroughs 1986) and washed twice with distilled water for 10 min. Seeds were then germinated in 120 mm diameter sterilized Petri dishes.…”

Section: Germination Trialmentioning

confidence: 99%

Genotypic variation in tolerance to salinity of common beans cultivated in Western Cameroon as assessed at germination and during early seedling growth

et al. 2017

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Open Agriculture. 2017; 2: 600-610 bean genotypes and most of their interactions. Significant correlations were found between all germination variables and between most growth parameters. From the effect of salt application, the common bean genotypes namely KEB-CB049, KEB-CB053 and Mac-33 were the most tolerant while KEB-CB055 and KEB-CB050 were the most sensitive. The results confirm that there is genotypic variation in salinity tolerance and that the most tolerant genotypes should be further explored in selection programs, with the aim that they should be promoted for cultivation in tropical zones affected by salinity.

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Section: Germination Trialmentioning

confidence: 99%

Genotypic variation in tolerance to salinity of common beans cultivated in Western Cameroon as assessed at germination and during early seedling growth

et al. 2017

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“…Healthy and uniform cowpea seeds of each accession were surface-sterilized with 7% sodium hypochlorite (NaOCl) for 20 min (Sauer and Burroughs, 1986) and washed twice with distilled water for 10 min. Seeds were then germinated in 90 mm diameter sterilized Petri dishes.…”

Section: Nacl Solutions and Seed Treatmentsmentioning

confidence: 99%

Salinity tolerance at germination of some main cultivated cowpea (Vigna unguiculata) genotypes from Western Cameroon

2017

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This study investigates the effect of salt (NaCl) on the germination of seeds of 10 genotypes of cowpea (Vigna unguiculata). The experiment carried out on petri dishes was done using a completely randomized design comprising three replications. The germination of the cultivars was studied using distilled water as control and under osmotic potentials of 50, 100, 150 and 200mM NaCl. The results indicated that the Mean Germination Time (MGT), Time to 50% Germination (T50), Germination Index (GI), Coefficient of Velocity of Germination (CVG), and Germination Percentage (GP), varied between 2.33 and 5.51 days; 2.83 and 14.87 days; 16.66 and 1.63; 0.53 and 0.11; 87.11 and 27.98% respectively. The variables GI, CVG and GP tended to decrease with increasing NaCl concentration while MGT and T50 tended to increase with increasing salt concentrations. For all the germination variables, significant differences at 0.001 probability levels were found among salinity treatments, cowpea genotypes and their interaction. Four genotypes (KEB-CP118, OU59A, OU100 and ICV12) were tolerant to salt stress and performed well under salinity conditions. Four other genotypes were found to be moderately tolerant (ET11, NO1036, MTA22 and KEB-CP009) and two genotypes (KEB-CP098 and NO193) were susceptible to salt stress and were most affected by salinity. At 0.001 probability level, positive correlations were observed between GP and GI (r = 0.792); GP and CVG (r = 0.774); GI and CVG (r = 0.955) and negative correlations were observed between GP and T50 (r = -0.845); GI and MGT (r = -0.894); CVG and MGT (r = -0.895).

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“…A few drops of Tween 20 (Sigma Aldrich, USA) solution were added with the sterilization solution to wash the seeds in the 250 mL beaker. The seeds held in the mesh were then surface sterilized by adding sterilization solution followed by soaking for 20 min (Sauer and Burroughs, 1986). The seeds were then washed three times with sterile water to remove the detergent.…”

Section: Seed Sterilization For the In Vitro Techniquementioning

confidence: 99%

A timeline for Eustoma grandiflorum seedling production based on an in vitro germination protocol

Roni¹,

Islam²,

Shimasaki³

2017

Plant Omics

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Eustoma (Eustoma grandiflorum) is a high-rank ornamental cut flower, and propagation of plant material by seed is an important approach for high-volume production of Eustoma. Successful propagation using different parts of the Eustoma plant has been reported in numerous studies; however, to date there have been no studies comparing the timeline differences between in vitro and conventional germination growth methods. The objective of this study was to develop a convenient and straightforward in vitro protocol that decreases the lag time to germination and the seedling development periods. Seed germination and seedling growth was studied in Eustoma (Voyage type 2 pink) seeds using two different techniques, in vitro germination and conventional sowing in soil. Seeds sown under in vitro conditions exhibited faster (6 days) germination compared with seeds sown directly in soil (11 days). Halfstrength Murashige and Skoog medium in vitro-grown seedlings (7 weeks) were more vigorous and produced true leaves earlier compared with conventionally-grown seedlings (15 weeks). In addition, when Eustoma seeds were sown in vitro, germination could be more easily tracked and visually compared with seeds sown in soil media. Therefore, the in vitro seed germination protocol developed in this study is straightforward, reproducible, and will amenable to facilitate efficient seedling production of Eustoma for commercial applications.

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Disinfection of Seed Surfaces with Sodium Hypochlorite

Cited by 191 publications

References 0 publications

Genotypic variation in tolerance to salinity of common beans cultivated in Western Cameroon as assessed at germination and during early seedling growth

Genotypic variation in tolerance to salinity of common beans cultivated in Western Cameroon as assessed at germination and during early seedling growth

Salinity tolerance at germination of some main cultivated cowpea (Vigna unguiculata) genotypes from Western Cameroon

A timeline for Eustoma grandiflorum seedling production based on an in vitro germination protocol

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