66506 (third author). Contribution 78-321-J, Kansas Agricultural Experiment Station, Manhattan 66502. Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other suitable products.

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10 µg/g, respectively, which were about six times as great as those found in heavily weathered sorghum grain at harvest. Two of four isolates of Alternaría alternata were toxic when corn-rice-AZfemana culture constituted half the diet for chicks and rats. Although all the isolates produced AME, AOH, and ALT, the two that also produced tenuazonic acid and altertoxin I were lethal. Tenuazonic acid was not found in any of the 12 samples of weathered sorghum grain analyzed by gas chromatography.When grain sorghum, Sorghum bicolor (L.) Moench, is exposed to wet weather before harvest, the seeds are often discolored by fungal growth. Two Alternaría metabolites, alternariol (AOH) and alternariol monomethyl ether

Metabolites, alternariol (AOH) and alternariol monomethyl ether (AME), were isolated from discolored, weathered grain sorghum that had been invaded by Alternaría sp. These compounds were found in 1973 spring-harvested (overwintered) sorghum and in weathered sorghum har-vested in the fall of 1973. Thin-layer chromatograms and fluorescence of AME and zearalenone are similar. Means of avoiding confusion of AME, AOH, and other fluorescent substances in grain sorghum with zearalenone and aflatoxin are discussed.

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