Disk-shaped immobilized multicomponent reagent for bioluminescent analyses: Correlation between activity and composition

Esimbekova, Elena N.; Kratasyuk, Valentina A.; Torgashina, I. G.

doi:10.1016/j.enzmictec.2006.05.003

Cited by 22 publications

(18 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Adding polysaccharides such as 0.5% maltose or 0.3% starch enhanced significantly the tolerance of T4 phage to the air-drying effect. This is consistent with previous reports that mentioned the usefulness of different polysaccharides in enhancing the stability of various biologically active compounds against desiccation (7,14,21). This study found that freeze-drying was an efficient method to dry phages with little decrease in phage activity, which would help in developing different paper-coating approaches for the commercial manufacture of phage-containing bioactive membranes.…”

Section: Discussionsupporting

confidence: 94%

Biocontrol of Listeria monocytogenes and Escherichia coli O157:H7 in Meat by Using Phages Immobilized on Modified Cellulose Membranes

Anany

Chen

Pelton

et al. 2011

Appl Environ Microbiol

202

140

View full text Add to dashboard Cite

The ability of phages to specifically interact with and lyse their host bacteria makes them ideal antibacterial agents. The range of applications of bacteriophage can be extended by their immobilization on inert surfaces. A novel method for the oriented immobilization of bacteriophage has been developed. The method was based on charge differences between the bacteriophage head, which exhibits an overall net negative charge, and the tail fibers, which possess an overall net positive charge. Hence, the head would be more likely to attach to positively charged surfaces, leaving the tails free to capture and lyse bacteria. Cellulose membranes modified so that they had a positive surface charge were used as the support for phage immobilization. It was established that the number of infective phages immobilized on the positively charged cellulose membranes was significantly higher than that on unmodified membranes. Cocktails of phages active against Listeria or Escherichia coli immobilized on these membranes were shown to effectively control the growth of L. monocytogenes and E. coli O157:H7 in ready-to-eat and raw meat, respectively, under different storage temperatures and packaging conditions. The phage storage stability was investigated to further extend their industrial applications. It was shown that lyophilization can be used as a phage-drying method to maintain their infectivity on the newly developed bioactive materials. In conclusion, utilizing the charge difference between phage heads and tails provided a simple technique for oriented immobilization applicable to a wide range of phages and allowed the retention of infectivity.

show abstract

Section: Discussionsupporting

confidence: 94%

Biocontrol of Listeria monocytogenes and Escherichia coli O157:H7 in Meat by Using Phages Immobilized on Modified Cellulose Membranes

Anany

Chen

Pelton

et al. 2011

Appl Environ Microbiol

202

140

View full text Add to dashboard Cite

show abstract

“…Currently biological polymers gelatin and starch are widely used in biotechnology in media for enzyme immobilization [1][2][3][4][5] or in responsive polymer systems for controlled drug delivery [6]. Gelatin and starch gels meet many requirements for media suitable for immobilization of the biological components (high mechanical strength, porosity, etc.).…”

Section: Introductionmentioning

confidence: 99%

“…Important physico-chemical characteristic of gelatin and starch solutions and gels is the increased viscosity, which affects the rate of diffusion of both the immobilized (entrapped) components and analyte molecules. Viscosity change can influence the functional activity of proteins included into the gel, as well as the rate of drug release [2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

“…The aim of the current study was to analyze the relationship between macro-and microviscosity of solutions and gels based on potato starch and gelatin that were used for immobilization of enzymes from luminous bacteria [2][3][4][5]. The increasing enzyme activity and stability were revealed at certain concentrations of biopolymers [2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

“…The increasing enzyme activity and stability were revealed at certain concentrations of biopolymers [2][3][4][5]. In this context, information on media viscosity is an important element in understanding the mechanisms underlying observed effects.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Contrasting relationship between macro- and microviscosity of the gelatin- and starch-based suspensions and gels

2016

View full text Add to dashboard Cite

The problem of correlation between rheological properties in macroand micro-scales of media with biopolymers of polypeptide (gelatin) and polysaccharide (starch) nature is investigated. The viscosity of the biopolymer solutions with concentrations 0.5-5 wt% was estimated by standard rotational rheometry technique and with fluorescent molecular rotor at 15-50°C. Opposite trends were observed for relationship between microviscosity g m and macroviscosity g for two biopolymers: g m \ \ g for gelatin and g m [ [ g for starch solutions. The temperature dependence of g m followed the monoexponential decay law in all samples over the whole temperature range indicating insensitivity of microviscosity to gel mesh melting under heating. The dissimilarity of macro-and micro-rheological properties of gelatin and starch-containing media is discussed in terms of difference in architecture of the gels.

show abstract

Bioluminescent enzyme assay for the indication of plant stress in enclosed life support systems

et al. 2011

View full text Add to dashboard Cite

The application of bioluminescent sensors for monitoring key metabolites and enzymes that are indicators of stress in plants is demonstrated. The sensitivity of bioluminescent assay for NAD(P)H and NAD(P)(+) was about 0.5 and 1 nmol, respectively. The levels of NAD(P)H and NAD(P)(+) in radish (Raphanus sativus) root extracts from controls and from stress-induced conditions were compared. To induce environmental stress, the plants were grown in enclosed environmental chambers with low pressure (9 or 32 kPa), high humidity (>80%) and low oxygen partial pressure (down to 3.3-6.5 kPa). The concentrations of NAD(P)(+) and NAD(P)H in plants varied under stress conditions. Decreasing both total pressure from 101.5 to 32 or 9 kPa and partial pressure of oxygen increased the ratio of NAD(P)(+) /NAD(P)H from 0.2 to 4 or 6, respectively. The increase in this ratio suggests that plants are undergoing stress in these hypobaric environments. The developed bioluminescent assay for quantification of pyridine nucleotides in plant tissues is rapid, low-cost and easily performed.

show abstract

Disk-shaped immobilized multicomponent reagent for bioluminescent analyses: Correlation between activity and composition

Cited by 22 publications

References 8 publications

Biocontrol of Listeria monocytogenes and Escherichia coli O157:H7 in Meat by Using Phages Immobilized on Modified Cellulose Membranes

Biocontrol of Listeria monocytogenes and Escherichia coli O157:H7 in Meat by Using Phages Immobilized on Modified Cellulose Membranes

Contrasting relationship between macro- and microviscosity of the gelatin- and starch-based suspensions and gels

Bioluminescent enzyme assay for the indication of plant stress in enclosed life support systems

Contact Info

Product

Resources

About