Disorders in structural-functional properties of erythrocytes in experimental acute destructive pancreatitis of alcohol etiology and their correction

Abstract

The effects of various combinations of pharmacological agents on parameters characterizing red blood cell (RBC) membrane proteins and lipids have been investigated in RBC isolated from Wistar male rats with acute destructive pancreatitis induced under conditions of forced alcoholization for 60 days. Administration of a combination of Hepon, Hypoxenum, and Phosphogliv normalized 22.5% of parameters altered change during development of acute destructive pancreatitis under conditions of chronic alcoholization of parameters, corrected towards normal values 42.5% of parameters (35% of parameters remained unchanged). Administration of Glutoxim, Mexidol and Heptral, was more effective: this combination normalized 50.0% of parameters studied, corrected towards normal values 37.5% of parameters, leaving unchanged only 12.5% of parameters studied.

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“…An increase in the number of right-wing Er histograms in all patients from the main group, regardless the level of PR, is associated with a probable (p <0.05) increase in median and mode values. The above mentioned features of the histogram mean that, in response to the effect of Al, Er of small size react primarily (their number decreases sharply), while the number of macrocellular forms gradually increases, which is typical of toxic reactions that are likely to develop as a result of the release of the "first-phase stress-reaction" cells from the reserve pool of total blood circulation (Konoplya et al, 2017). As the reserve capacity of Er decreases and the patients move to a lower level of the PR, the distribution of Ep becomes more extreme: the number of intervals increases, the volumes of the individual classes vary as a result of jumps, and the achievement of the values of the central parameters is wavelike (in the CG for a monotonously rising trend).…”

Proteins of allogeneic hepatocytes and pharmacological preparations for the correction of immunometabolic disorders in experimental liver pathology

“…According to the literature data and basing on the results obtained, we can summarize that in toxic liver dam- age of different etiologies, the neutrophil respiratory burst system is activated, which results in a shift of pro-and antioxidants balance towards weakening the latter ones, that is, towards an increase in LPO processes in cellular membranes, undermining them not only in hepatocytes, but also in erythrocytes (Shikalova et al 2012, Zabrodskiĭ et al 2015, Konoplja et al 2017. The transplantation of xeno-and, especially, allogeneic hepatocytes, to the recipients suffering from ATLD, ALI and CAI limits the processes of free-radical oxidation at the systemic and local (erythrocytes) levels, the systemic inflammatory response at the level of immunity mechanisms, and has significant positive effects on restoring the hepatocyte functional activity and endoglobular metabolism.…”

“…2009, Minasyan 2014, He et al 2017, Pretini et al 2019. There are a large number of experimental and clinical papers on the correction of liver dysfunctions, including the use of cellular technologies (Trubitsyna et al 2016, Danilova et al 2020; there are studies on oxidative, immune and erythrocytic disorders and their correction in liver pathology (Konoplja et al 2017), but in fact there are no papers concerning the corrective effect of xeno-and allogeneic cells and transplantation of their cultural humoral factors.…”

“…There is a large number of experimental and clinical works in the literature on the correction of liver dysfunctions, there are also studies concerning oxidative, immune and erythrocyte disorders and their correction in liver pathologies (Manzini еt al. 2013, Thursz et al 2015, Konoplja et al 2017, Suyavaran 2017.…”

The use of immunomodulators, antioxidants and hepatoprotectors for the correction of the liver, erythrocites and the immune system disorders in chronic ethanol intoxication

“…Основными функциями эритроцитов циркулирующей крови являются: питательная, защитная, буферная, газотранспортная, гуморальная, транспортная, гомеостатическая, дезинтоксикационная, участие в метаболизме гормонов, лекарственных препаратов, регуляция сосудистого тонуса. Известна важная роль эритроцитов в поддержании иммунного гомеостаза при патологии печени и поджелудочной железы [12,13]. В условиях хронической алкогольной интоксикации (ХАИ) изменениям иммунного гомеостаза, структурно-функциональных свойств эритроцитов, роли оксидантного стресса посвящено небольшое количество исследований [14,15].…”