The effects of various combinations of pharmacological agents on parameters characterizing red blood cell (RBC) membrane proteins and lipids have been investigated in RBC isolated from Wistar male rats with acute destructive pancreatitis induced under conditions of forced alcoholization for 60 days. Administration of a combination of Hepon, Hypoxenum, and Phosphogliv normalized 22.5% of parameters altered change during development of acute destructive pancreatitis under conditions of chronic alcoholization of parameters, corrected towards normal values 42.5% of parameters (35% of parameters remained unchanged). Administration of Glutoxim, Mexidol and Heptral, was more effective: this combination normalized 50.0% of parameters studied, corrected towards normal values 37.5% of parameters, leaving unchanged only 12.5% of parameters studied.

ГБОУ ВПО «Курский государственный медицинский университет» Минздрава РФ, Курск, Российская Федерация Цель -изучение корригирующих эффектов аллогенных и ксеногенных гепатоцитов на метаболиче ские нарушения в условиях острого токсического поражения печени. Материал и методы. Исследования про-ведены на 75 половозрелых крысах-самцах Вистар массой 120-160 г, 15 крысах и 25 мышах на 5-6-й день после рождения. Острое токсическое поражение печени (ОТПП) моделировали путем внутримышечного введения четыреххлористого углерода в дозе 3 мл/кг в виде 50% раствора в оливковом масле пятикратно с интервалом 24 ч. Выделение ксеногенных (мышиных) и аллогенных гепатоцитов от новорожденных животных производилось по методике M.N. Berry, D.S. Friend. Суспензию клеток готовили ежедневно и вводили в концентрации 2 × 10 6 /кг реципиентам с ОТПП внутрибрюшинно, пятикратно, через 24 ч, одновременно с первой инъекцией гепатотропного яда. Результаты. Интоксикация четыреххлористым углеродом вызывает развитие биохимических синдромов поражения печени, активацию функционально-метаболической активности нейтрофилов периферической крови и свободно-радикального окисления, нарушает внутриэритроцитарный метаболизм. Введение аллогенных гепатоцитов реципиентам с токси-ческой гепатопатией более эффективно по сравнению с ксеногенными гепатоцитами, корригирует сис-темные и локальные метаболические нарушения, возникающие вследствие воздействия гепатотропного яда. Заключение. Трансплантация ксеногенных гепатоцитов, в большей степени аллогенных, в условиях ОТПП является эффективным средством по восстановлению функционально-метаболической активнос-ти гепатоцитов, нейтрофилов и эритроцитов. Ключевые слова: трансплантация ксено-и аллогенных гепатоцитов, острое токсическое поражение печени, коррекция метаболических нарушений. IMMUNE AND METABOLIC DISTURBANCES IN EXPERIMENTAL ACUTE TOXIC HEPATITIS: CORRECTION BY XENOGENIC AND ALLOGENIC HEPATOCYTES A.I. Konoplya, E.S. Litvinova, N.A. Bystrova, M.S. Razumova, T.V. ChuyevaKursk State Medical University, Ministry of Healthcare of the Russian Federation, Kursk, Russian Federation Aim. To study the corrective effects of allogeneic and xenogeneic hepatocytes on metabolic disturbances in acute liver toxicity. Material and methods. Investigations were carried out on 75 adult male Wistar rats weighing 120-160 g, 15 rats and 25 mice on the 5-6th days after birth. Acute toxic hepatitis (ATH) was modeled by intramuscular injection of carbon tetrachloride at a dose of 3 ml / kg as a 50% solution in olive oil, fi ve times at 24-hour intervals. Isolating xenogeneic (mouse) and allogeneic hepatocytes was performed by method of Berry M.N., Friend D.S. The cell suspension was prepared daily and administered at a concentration of 2 × 10 6 /kg in recipients with ATH intraperitoneally, fi ve times at 24-hour intervals, simultaneously with the fi rst injection of hepatotropic poison. Results. Intoxication by carbon tetrachloride causes development of the biochemical syndromes of liver damage, activation of the functional metabolic activ...

Introduction: The relationship of numerous metabolic shifts, disorders of hepatocytes functional activity resulting from hypoxia and toxic liver damage with the function of the immune system has not been sufficiently studied so far, nor have the most effective methods of pharmacological correction been established. Materials and Methods: The studies were carried out on 603 mature male Wistar rats and 45 mice. Acute toxic liver damage (ATLD) was modeled by intramuscular introduction of carbon tetrachloride; acute liver ischemia (ALI) was caused by clamping the hepatoduodenal ligament for 20 minutes; chronic alcohol intoxication (CAI) was modeled by forced intragastric administration of 20% ethanol solution for 60 days. Isolation of xenogeneic (murine) and allogeneic (rat) hepatocytes from newborn mice and rats was carried out according to the method of Berry and Friend (1969); culture fluid of hepatocytes and its protein fractions were prepared according to our developed techniques. The obtained biological material was intraperitoneally introduced into the rats with ATLD, ALI, and CAI. Results and Discussion: In all the models of the liver damage, there developed morphological and biochemical signs of the liver damage, impaired congenital and adaptive immunity, oxidative stress, increased lipid peroxidation processes. Conclusion: The introduction of allogeneic hepatocytes, culture fluid obtained on their basis,and proteins isolated from it with MW less than 130 kDa to the recipients with toxic and ischemic liver damage more effectively corrects the pathologic changes in the liver in comparison with xenogeneic hepatocytes, their culture fluid and pharmacological preparations (combinations of Essentiale N and Hypoxenum or Heptral and Mexicor).

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Difficulties in describing such notions as vernacular, common slang and slang and the reference of certain lexical units or texts to a particular language phenomenon stem from the variety of opinions and ways of defining each of them. These debatable questions have become more distinctive in Russian linguistics with the growing contacts with European linguistic schools and therefore copying the terms without adapting them to Russian theory of language. The using of these terms is becoming chaotic due to the fact that modern Russian linguistics often neglects the basic achievements of Soviet linguistics in the field of distinguishing language varieties. The article considers two approaches to the definition of vernacular, common slang and slang: from the point of view of their being fully functional language varieties and from the point of view of their functional facilities. As a result of the analysis of the data about language varieties, a conclusion is drawn about the common and different in these concepts. If these notions considered as similar to major language varieties such as standard language, standard colloquial speech and territorial dialects, then they cannot be called fully functional language varieties, since they have an extremely vague social base, they are characterized by a low degree of standardization, functional diversity and the intersection of their lexical content. Thus, it can be concluded that such language phenomena belong to the specific kind of language variations that are defined by their transitional nature. If functional facility of vernacular, common slang and slang is considered, it is worth noting that there are a lot of stylistically marked lexical units in slang. They are slightly less numerous in vernacular, and even less numerous in common slang. This peculiarity stems from the fact that common slang includes lexical units able to function as the fillers of the gaps in standard language, standard colloquial speech or territorial dialects. It should be noted that the lexical units of all three language phenomena are used in various communication situations even by the people who are well versed in the norms of the standard language. The fact that lexical units belong to vernacular, common slang or slang does not prevent well-educated speakers from using all stylistic functions of such units. Speakers who know the difference between standard and slang or vernacular words are able to vary these language tools to attract the attention of a certain category of people (for example, for advertising, communicating with youth, etc.).