Immune and Metabolic Disturbances in Experimental Acute Toxic Hepatitis: Correction by Xenogenic and Allogenic Hepatocytes

Конопля, А. И.; Литвинова, Е. С.; Быстрова, Н. А.; Разумова, М. С.; Чуева, Т. В.

doi:10.15825/1995-1191-2016-2-91-98

Cited by 4 publications

(4 citation statements)

References 3 publications

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“…The isolation of the "active" principle from the culture fluid of hepatocytes is promising. The reasons for this are the facts earlier obtained in our laboratory and proving that not only the transplantation of allogeneic intact hepatocytes, but also the introduction of culture fluid obtained on their basis to the recipients suffering from experimental acute liver hypoxia, acute toxic hepatitis caused by carbon tetrachloride and prolonged alcohol intoxication significantly reduces the development of immune-inflammatory syndrome in the liver, normalizes hepatocytes synthetic function, prevents the development of oxidative stress and innate immunity impairment (Konoplya et al 2016.…”

Section: Acute Liver Ischemiamentioning

confidence: 95%

“…To enhance our insight about the role of the liver in the diseases pathogenesis, to develop qualitative and quantitative assessment criteria of its morphological and functional indicators in dynamics is a difficult, multifaceted, but extremely urgent task for current knowledge (Chikoteev et al 2003, Konoplya et al 2016.…”

Section: Problemmentioning

confidence: 99%

“…Hepatocytes were prepared daily and immediately introduced after preparation. During all the procedures with the cell suspension, the temperature of the used medium 199 was 36-37 °С (Konoplya 1985, Konoplya et al 2016.…”

Section: Preparation Of Xenogeneic and Allogeneic Hepatocytesmentioning

confidence: 99%

“…One of the most common effects caused by xenobiotics intake to the body is the labialization of lysosomal membranes. This significantly increases the "leakage" of hydrolytic enzymes of lysosomes into the blood plasma (Zabrodskiĭ et al 2015, Konoplya et al 2016.…”

Section: Introductionmentioning

confidence: 99%

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Proteins of allogeneic hepatocytes and pharmacological preparations for the correction of immunometabolic disorders in experimental liver pathology

Литвинова¹,

Konoplya²,

Шульгинова³

et al. 2021

RRP

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Introduction: The relationship of numerous metabolic shifts, disorders of hepatocytes functional activity resulting from hypoxia and toxic liver damage with the function of the immune system has not been sufficiently studied so far, nor have the most effective methods of pharmacological correction been established. Materials and Methods: The studies were carried out on 603 mature male Wistar rats and 45 mice. Acute toxic liver damage (ATLD) was modeled by intramuscular introduction of carbon tetrachloride; acute liver ischemia (ALI) was caused by clamping the hepatoduodenal ligament for 20 minutes; chronic alcohol intoxication (CAI) was modeled by forced intragastric administration of 20% ethanol solution for 60 days. Isolation of xenogeneic (murine) and allogeneic (rat) hepatocytes from newborn mice and rats was carried out according to the method of Berry and Friend (1969); culture fluid of hepatocytes and its protein fractions were prepared according to our developed techniques. The obtained biological material was intraperitoneally introduced into the rats with ATLD, ALI, and CAI. Results and Discussion: In all the models of the liver damage, there developed morphological and biochemical signs of the liver damage, impaired congenital and adaptive immunity, oxidative stress, increased lipid peroxidation processes. Conclusion: The introduction of allogeneic hepatocytes, culture fluid obtained on their basis,and proteins isolated from it with MW less than 130 kDa to the recipients with toxic and ischemic liver damage more effectively corrects the pathologic changes in the liver in comparison with xenogeneic hepatocytes, their culture fluid and pharmacological preparations (combinations of Essentiale N and Hypoxenum or Heptral and Mexicor).

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Section: Acute Liver Ischemiamentioning

confidence: 95%

Section: Problemmentioning

confidence: 99%

Section: Preparation Of Xenogeneic and Allogeneic Hepatocytesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Proteins of allogeneic hepatocytes and pharmacological preparations for the correction of immunometabolic disorders in experimental liver pathology

Литвинова¹,

Konoplya²,

Шульгинова³

et al. 2021

RRP

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Relationship between metabolic and immunological disorders in experimental acute tetrachlomethane, ischemic and alcoholic liver disease

et al. 2020

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The aim of this study was to investigate the relationship of metabolic and immunological disorders in acute tetrachlomethane, ischemic and alcoholic liver damage modelled in adult Wistar male rats weighing 120-160 g. After evaluation of metabolic and immunological parameters at the local and systemic levels, and correlation analysis was used to establish the relationship between the dynamics of the indicators against the background of experimental pathology models. The close correlation between the studied immune and metabolic parameters recognized for the tetrachlomethane, ischemic and alcoholic liver damage shows the existing “tension” between the indicators of immune and metabolic status. Such close correlation between the studied immunological and metabolic parameters at the system and local levels can serve to assess the severity of the disease, its prognosis, treatment effectiveness and preventive measures.

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Relationship Between Metabolic and Immunological Indicators After Correction by Allogenic Hepatocytes in Acute Toxic, Ischemic and Alcohol Liver Injury

Литвинова

Konoplya

Холименко

et al. 2021

Toksikologičeskij vestnik

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Immune disorders arising from liver damage of various origins and the mechanisms of their development are still poorly understood. The functions of the immune system are carried out against the background of metabolic processes and their shifts caused by the action of various agents on the body, as well as liver cells - hepatocytes. Typical metabolic changes that occur when the liver is affected by various toxic factors are combined with certain features of metabolic disorders in certain organs and tissues, due to the specifics of their structural and functional organization, the nature of the inducing agent and the primary link of its effect on cells and the body as a whole. The relationship of numerous metabolic changes, violations of the functional activity of hepatocytes arising in such a pathology with the dysfunction of the immune system has not yet been sufficiently studied, as well as the most effective methods of correction have not been established. Currently, the issues of pathogenesis, diagnosis and treatment of acute liver diseases remain among the most relevant in medicine, both due to the complexity of diagnosis and the choice of optimal treatment methods, and the tendency towards an increase in the number of patients with these diseases. The emergence and development of cellular technologies have created serious scientific prerequisites in this area.

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Immune and Metabolic Disturbances in Experimental Acute Toxic Hepatitis: Correction by Xenogenic and Allogenic Hepatocytes

Cited by 4 publications

References 3 publications

Proteins of allogeneic hepatocytes and pharmacological preparations for the correction of immunometabolic disorders in experimental liver pathology

Proteins of allogeneic hepatocytes and pharmacological preparations for the correction of immunometabolic disorders in experimental liver pathology

Relationship between metabolic and immunological disorders in experimental acute tetrachlomethane, ischemic and alcoholic liver disease

Relationship Between Metabolic and Immunological Indicators After Correction by Allogenic Hepatocytes in Acute Toxic, Ischemic and Alcohol Liver Injury

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