Disposable amperometric immunosensing strips fabricated by Au nanoparticles-modified screen-printed carbon electrodes for the detection of foodborne pathogen Escherichia coli O157:H7

Gold Nanoparticles in Biosensing Analyses

“…In a fluorescent-based competition analyses, the binding affinity of the protein (Lectin Con A) against lyconanoparticles (AuNPs conjugated with underivatized mono-, oligo-and polysaccharides) was determined [50]. A wide range of additional protein biomarkers have been detected besides cancer biomarkers, including cholera toxin [55], inter-leukin [56] vascular endothelial growth factors [57], Annexin II and MUC5AC antigens [58], antigens of Schistosoma japonicum [59], Salmonella typhi [60,61], Escherichia coli O157:H7 [62] and osteoprotegerin [63]. AuNP-based electrochemical sandwich immunosensor was designed for the detection of fenolase (ENO1) antigen, a potential diagnostic marker for lung cancer, with a detection limit of 11.9 fg [64].…”

Nanomaterial-based sensors for detection of foodborne bacterial pathogens and toxins as well as pork adulteration in meat products

“…An E. coli O157:H7 eaeA gene-specific thiolated probe conjugated to PEB was exposed to E. coli gene fragment amplified by PCR and the resultant mass change was measured as frequency shift of PEB, with the detection limit obtained being 1.2 Â 10 2 CFU/mL in the linear working range of 10 2 e10 6 CFU/mL [20]. For detection of E. coli O157:H7 in milk, a disposable immunosensing strip containing double antibodies for indirect sandwich enzyme-linked immunoassay was fabricated by attaching 13-nm GNPs onto screen-printed carbon electrodes (SPCEs) [21]. The electrode was coupled with the first E.…”

Screen-printed integrated microsystem for the electrochemical detection of pathogens

“…The screen-printed microdevice is used as substrate for particle-bacteria immobilization as well as tunable low volume detection chamber (down to 10 L) and it incorporates the lysing material that aids releasing the detectable compounds in the vicinity of the electrode surface. The use of screen-printed materials as working electrode has been reported in pathogen detection devices [21,22]; however, in the work reported here the carbonbased screen-printed materials not only act as functional but also as structural component, improving the simplicity of the design and the ease of fabrication. In this work it is also shown the immo- bilization of paramagnetic particles by means of electrophoretic deposition, which consisted of successfully depositing epoxy particles with a paramagnetic core of iron oxide on the surface of an electrode by electrophoretic deposition.…”

“…Many works reporting electrochemically based bacteria detection rely on the detection of intracellular compounds or metabolytes excreted by the bacteria or directly on the detection of compounds released to the solution after the cell lysis [18,19]. Both electrochemical impedance and amperometric measurements are commonly used to quantify the pathogenic loads [5,8,11,13,20,21]. It becomes clear that electrochemistry is a well-reported method implemented in the quantification of bacterial loads.…”