Disposable microfluidic chip for rapid pathogen identification with DNA microarrays

Peham, Johannes R.; Recnik, Lisa-Maria; Grienauer, Walter; Vellekoop, Michael J.; Nöhammer, Christa; Wiesinger-Mayr, Herbert

doi:10.1007/s00542-011-1401-0

Cited by 11 publications

(6 citation statements)

References 8 publications

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“…Over the past decades, a variety of methods, such as polymerase chain reaction (PCR)-based methods, [3][4][5][6][7] DNA microarrays, [8][9][10] DNA sequencing technology 11,12 enzymelinked immunosorbent assay (ELISA), 13,14 staining, 15 isolation, 16 cell culture, 17 and so on, have been employed for pathogen detection. Although the aforementioned DNA-based methods have been widely used for efficient pathogen identification, they cannot detect pathogenic microorganisms directly.…”

Section: Introductionmentioning

confidence: 99%

A PDMS/paper/glass hybrid microfluidic biochip integrated with aptamer-functionalized graphene oxide nano-biosensors for one-step multiplexed pathogen detection

et al. 2013

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Infectious pathogens often cause serious public health concerns throughout the world. There is an increasing demand for simple, rapid and sensitive approaches for multiplexed pathogen detection. In this paper we have developed a polydimethylsiloxane (PDMS)/paper/glass hybrid microfluidic system integrated with aptamer-functionalized graphene oxide (GO) nano-biosensors for simple, one-step, multiplexed pathogen detection. The paper substrate used in this hybrid microfluidic system facilitated the integration of aptamer biosensors on the microfluidic biochip, and avoided complicated surface treatment and aptamer probe immobilization in a PDMS or glass-only microfluidic system. Lactobacillus acidophilus was used as a bacterium model to develop the microfluidic platform with a detection limit of 11.0 cfu mL−1. We have also successfully extended this method to the simultaneous detection of two infectious pathogens - Staphylococcus aureus and Salmonella enterica. This method is simple and fast. The one-step ‘turn on’ pathogen assay in a ready-to-use microfluidic device only takes ~10 min to complete on the biochip. Furthermore, this microfluidic device has great potential in rapid detection of a wide variety of different other bacterial and viral pathogens.

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Section: Introductionmentioning

confidence: 99%

A PDMS/paper/glass hybrid microfluidic biochip integrated with aptamer-functionalized graphene oxide nano-biosensors for one-step multiplexed pathogen detection

et al. 2013

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“…1.12) (Liu et al ., 2001;Yang et al ., 2002;Hashimoto et al ., 2004;Chan et al ., 2008;Cooney et al ., 2012;Peham et al ., 2012).…”

Section: Polycarbonatementioning

confidence: 94%

Materials and methods for the microfabrication of microfluidic biomedical devices

Wu¹,

Rezai²,

Hsu³

et al. 2013

Microfluidic Devices for Biomedical Applications

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“…The failure in treatment of the bacterial infections can be detrimental and can even lead to sepsis and death. Currently, the widely used diagnosis technique are polymerase chain reaction (PCR)-based methods, DNA microarrays, DNA sequencing technology, ELISA, staining, isolation, cell culture, and biochemical tests [4][5][6][7][8]. Most of these methods are quite complex, time consuming, involve multiple steps and require costly and high-precision instruments that rely on cumbersome procedures.…”

Section: Introductionmentioning

confidence: 99%

Graphene-Based Sensor for Detection of Bacterial Pathogens

Pandit

Chen

et al. 2021

Sensors

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Microbial colonization to biomedical surfaces and biofilm formation is one of the key challenges in the medical field. Recalcitrant biofilms on such surfaces cause serious infections which are difficult to treat using antimicrobial agents, due to their complex structure. Early detection of microbial colonization and monitoring of biofilm growth could turn the tide by providing timely guidance for treatment or replacement of biomedical devices. Hence, there is a need for sensors, which could generate rapid signals upon bacterial colonization. In this study, we developed a simple prototype sensor based on pristine, non-functionalized graphene. The detection principle is a change in electrical resistance of graphene upon exposure to bacterial cells. Without functionalization with specific receptors, such sensors cannot be expected to be selective to certain bacteria. However, we demonstrated that two different bacterial species can be detected and differentiated by our sensor due to their different growth dynamics, adherence pattern, density of adhered bacteria and microcolonies formation. These distinct behaviors of tested bacteria depicted distinguishable pattern of resistance change, resistance versus gate voltage plot and hysteresis effect. This sensor is simple to fabricate, can easily be miniaturized, and can be effective in cases when precise identification of species is not needed.

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Disposable microfluidic chip for rapid pathogen identification with DNA microarrays

Cited by 11 publications

References 8 publications

A PDMS/paper/glass hybrid microfluidic biochip integrated with aptamer-functionalized graphene oxide nano-biosensors for one-step multiplexed pathogen detection

A PDMS/paper/glass hybrid microfluidic biochip integrated with aptamer-functionalized graphene oxide nano-biosensors for one-step multiplexed pathogen detection

Materials and methods for the microfabrication of microfluidic biomedical devices

Graphene-Based Sensor for Detection of Bacterial Pathogens

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