Microbial sulfate reduction (MSR) has increasingly been investigated for its potential to immobilize metals and reduce their bioavailability while also increasing the pH of acid mine drainage (AMD; e.g., Nielsen et al., 2018). The process involves microbes (bacteria and archaea) converting sulfate into sulfide that together with toxic dissolved metals precipitate into less mobile forms. Laboratory bioreactor experiments on MSR show a metal retention of 70% or more under favorable conditions (e.g., Sinharoy et al., 2020;Zhang & Wang, 2016). The activity depends on several factors, such as (bioavailable) carbon and sulfate supply, oxygen level, pH, and temperature (Xu & Chen, 2020). MSR has also been observed in the field at certain locations and time periods, for example, in individual wetlands or near tailing deposits at particular points of time (Mandernack et al., 2000;Praharaj & Fortin, 2004). Recently, Fischer, Jarsjö, et al. (2022) additionally showed evidence of ongoing and considerable MSR in multiple locations (so-called "hot spots") within an AMD-impacted catchment (Imetjoki, Northern Sweden), which is essential if MSR is to be used as an effective mitigation solution for spatially extensive mining sites and their downstream regions. However, large knowledge gaps remain regarding catchment-scale MSR in freshwater systems, where specific catchment and seasonal conditions could differ substantially from site to site. It is therefore not known to what extent MSR more generally could provide a basis for viable bioremediation, for instance, as part of nature-based solutions for sites impacted by (acid) mine drainage across the world. This includes the Arctic, which counts as one of the world's larger mining regions with numerous examples of largescale mine drainage development, and where cold conditions and pronounced seasonality may hamper the activity of freshwater sulfur-reducing microbes (SRM).Current evidence shows that point locations which are relatively favorable for MSR contain soil and sediments with sufficiently high organic matter content to support the metabolism of SRM, and that they are associated with