Disproportionation of inorganic sulfur compounds by a novel autotrophic bacterium belonging to Nitrospirota

Umezawa, Kazuhiro; Kojima, Hisaya; Kato, Yukako; Fukui, Manabu

doi:10.1016/j.syapm.2020.126110

Cited by 51 publications

(51 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sulfomarinibacter MAGs encoded rhodonaselike TusA and DsrE2, which act as sulfur-trafficking proteins in reverse-Dsr harboring sulfur-oxidizing bacteria, i.e., they help deliver sulfur to DsrABC for oxidation [126]. Interestingly, the "YTD gene clusters" that encode these enzymes are also common in genomes of anaerobic elemental sulfur-reducing and/or -disproportionating bacteria that have Dsr, and are suggested to be genetic indicators for disproportionation potential among these anaerobes [97]. The TusA proteins from Ca.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Novel taxa of Acidobacteriota implicated in seafloor sulfur cycling

et al. 2021

View full text Add to dashboard Cite

Acidobacteriota are widespread and often abundant in marine sediments, yet their metabolic and ecological properties are poorly understood. Here, we examined metabolisms and distributions of Acidobacteriota in marine sediments of Svalbard by functional predictions from metagenome-assembled genomes (MAGs), amplicon sequencing of 16S rRNA and dissimilatory sulfite reductase (dsrB) genes and transcripts, and gene expression analyses of tetrathionate-amended microcosms. Acidobacteriota were the second most abundant dsrB-harboring (averaging 13%) phylum after Desulfobacterota in Svalbard sediments, and represented 4% of dsrB transcripts on average. Meta-analysis of dsrAB datasets also showed Acidobacteriota dsrAB sequences are prominent in marine sediments worldwide, averaging 15% of all sequences analysed, and represent most of the previously unclassified dsrAB in marine sediments. We propose two new Acidobacteriota genera, Candidatus Sulfomarinibacter (class Thermoanaerobaculia, “subdivision 23”) and Ca. Polarisedimenticola (“subdivision 22”), with distinct genetic properties that may explain their distributions in biogeochemically distinct sediments. Ca. Sulfomarinibacter encode flexible respiratory routes, with potential for oxygen, nitrous oxide, metal-oxide, tetrathionate, sulfur and sulfite/sulfate respiration, and possibly sulfur disproportionation. Potential nutrients and energy include cellulose, proteins, cyanophycin, hydrogen, and acetate. A Ca. Polarisedimenticola MAG encodes various enzymes to degrade proteins, and to reduce oxygen, nitrate, sulfur/polysulfide and metal-oxides. 16S rRNA gene and transcript profiling of Svalbard sediments showed Ca. Sulfomarinibacter members were relatively abundant and transcriptionally active in sulfidic fjord sediments, while Ca. Polarisedimenticola members were more relatively abundant in metal-rich fjord sediments. Overall, we reveal various physiological features of uncultured marine Acidobacteriota that indicate fundamental roles in seafloor biogeochemical cycling.

show abstract

Section: Discussionmentioning

confidence: 99%

“…"YTD gene clusters" encoding sulfur-trafficking rhodonase-like proteins [97] were identified among Ca. Sulfomarinibacter MAGs.…”

Section: Marine Acidobacteriota Use Tetrathionate and Potentially Also Other Sulfur-cycle Intermediatesmentioning

confidence: 99%

Novel taxa of Acidobacteriota implicated in seafloor sulfur cycling

et al. 2021

View full text Add to dashboard Cite

show abstract

“…In the 2 # chamber, the abundances of Planctomycetota , which mainly include anaerobic bacteria 22 and Deinococcota increased. After inputting electric currents, Nitrospirota including nitrifying bacteri a 23 became one of main phyla (7.67%–19.23%) in the 3 # chamber. The abundances of Desulfobacterota including SRB increased from 1.34% to 8.38% in the 4 # chamber.…”

Section: Resultsmentioning

confidence: 99%

Bioelectrochemical sulfate reduction enhanced nitrogen removal from industrial wastewater containing ammonia and sulfate

Ren

Zheng

et al. 2021

AIChE Journal

View full text Add to dashboard Cite

Low organic carbon‐to‐nitrogen ratio and existing sulfate (SO42−) in industrial wastewater limited nitrogen removal. Coupling SO42− reduction with sulfide autotrophic denitrification provides a novel strategy. Herein, bioelectrochemical sulfate reduction was coupled with heterotrophic sulfate reduction to drive sulfide autotrophic denitrification. In this coupled system, total nitrogen (TN) removal efficiency was increased from ~25% to ~85% by inputting −45 mA electricity. With the help of supplying electrons to denitrification through SO42− reduction, coulomb efficiency was improved to 61.5%. Also, bioelectrochemical sulfate reduction could improve sulfur recovery and thus increase TN removal efficiency. Furthermore, through tuning turnover numbers of SO42−, high TN removal efficiency can be obtained at various concentrations of SO42−. Moreover, main functional bacteria in this system were identified. Finally, ~75% TN removal efficiency was achieved with real wastewater in this system. Overall, this work offered a new approach for efficient nitrogen removal from industrial wastewater containing SO42−.

show abstract

“…Alternatively, identifying robust and consistent genomic markers to distinguish MSR from MSD may allow more accurate screening of the metabolic capacity of microorganisms from genome content alone in the absence of characterized isolates. Such markers have not yet been identified but are a target of active investigation (e.g., Umezawa et al, 2020 ). Finally, purely phylogenetic approaches to understanding the evolution of sulfur cycling in the Desulfobulbales provide an understanding of the timing of these processes only in relative evolutionary time.…”

Section: Discussionmentioning

confidence: 99%

Expanded Genomic Sampling Refines Current Understanding of the Distribution and Evolution of Sulfur Metabolisms in the Desulfobulbales

2021

View full text Add to dashboard Cite

The reconstruction of modern and paleo-sulfur cycling relies on understanding the long-term relative contribution of its main actors; these include microbial sulfate reduction (MSR) and microbial sulfur disproportionation (MSD). However, a unifying theory is lacking for how MSR and MSD, with the same enzyme machinery and intimately linked evolutionary histories, perform two drastically different metabolisms. Here, we aim at shedding some light on the distribution, diversity, and evolutionary histories of MSR and MSD, with a focus on the Desulfobulbales as a test case. The Desulfobulbales is a diverse and widespread order of bacteria in the Desulfobacterota (formerly Deltaproteobacteria) phylum primarily composed of sulfate reducing bacteria. Recent culture- and sequence-based approaches have revealed an expanded diversity of organisms and metabolisms within this clade, including the presence of obligate and facultative sulfur disproportionators. Here, we present draft genomes of previously unsequenced species of Desulfobulbales, substantially expanding the available genomic diversity of this clade. We leverage this expanded genomic sampling to perform phylogenetic analyses, revealing an evolutionary history defined by vertical inheritance of sulfur metabolism genes with numerous convergent instances of transition from sulfate reduction to sulfur disproportionation.

show abstract

Disproportionation of inorganic sulfur compounds by a novel autotrophic bacterium belonging to Nitrospirota

Cited by 51 publications

References 45 publications

Novel taxa of Acidobacteriota implicated in seafloor sulfur cycling

Novel taxa of Acidobacteriota implicated in seafloor sulfur cycling

Bioelectrochemical sulfate reduction enhanced nitrogen removal from industrial wastewater containing ammonia and sulfate

Expanded Genomic Sampling Refines Current Understanding of the Distribution and Evolution of Sulfur Metabolisms in the Desulfobulbales

Contact Info

Product

Resources

About