Disruption of a gene encoding a hypothetical secreted protein from<i>Sclerotinia sclerotiorum</i>reduces its virulence on canola (<i>Brassica napus</i>)

Liang, Yue; Yajima, William; Davis, Maria R.; Kav, Nat N. V.; Strelkov, Stephen E.

doi:10.1080/07060661.2012.745904

Cited by 34 publications

(28 citation statements)

References 20 publications

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“…The virulence results warrant that the lesion diameter of the ∆bchp mutant is substantially lower than WT and ∆bchp-C complementary strains implying the particular role of the BcHP gene in the host infection. These results are inconsistent with the previously published report by Liang et al (2013) reporting that the pathogenicity of knock-out mutants of (ssv263) gene encoding a hypothetical secreted protein in S. sclerotiorum is significantly reduced compared to WT. Similarly, the deletion mutant of extracellular polysaccharide and virulence-related gene (epv) is one of six genes encoding a hypothetical protein in Xanthomonas oryzae pv.…”

Section: Figcontrasting

confidence: 57%

“…This gene show similarity to genes that code for proteins involved in pathogenicity in other fungi [e.g. (ssv263) encoding a hypothetical secreted protein which is required for full pathogenicity in Sclerotinia sclerotiorum] (Liang et al, 2013). To confirm whether the identified gene is exactly responsible for the observed phenotypes of the T-DNA integration mutant, the knock-out and the complementary of the identified gene in the wild-type strain could be performed as described by (Michielse et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Identification of new pathogenicity related to the gene encoding hypothetical protein in the gray mold fungus (Botrytis cinerea)

DafaAlla¹,

Abdalla²,

Algaili³

et al. 2017

Aust J Crop Sci

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Botrytis cinerea is an important necrotrophic fungus that causes gray mold disease in various important crop plants, leading to substantial economic losses. Herein, Agrobacterium tumefaciens-mediated transformation (ATMT) technique was used to create an insertional mutant library of B. cinerea to investigate its pathogenicity-related genes. Among 1,734 transformations only 46 mutants decreased the pathogenicity on tomato leaves. C2M52 is one of the 46 mutants that its pathogenicity reduction was analyzed deeply. The T-DNA integrated into the promoter region of the hypothetical protein (BcHP) gene. The gene replacement approach via homologous recombination was used to generate knock-out mutant (△bchp) and its functional complementary (△bchp-C) strains. The results warranted that the △bchp mutant strain displayed a significant difference in the virulence on tomato leaves relative to the wild type. These results were consistent with the attenuated pathogenicity caused by T-DNA integration mutant (C2M52). That subsequently revealed the crucial role of BcHP gene in the pathogenicity of B. cinerea.

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Section: Figcontrasting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Identification of new pathogenicity related to the gene encoding hypothetical protein in the gray mold fungus (Botrytis cinerea)

DafaAlla¹,

Abdalla²,

Algaili³

et al. 2017

Aust J Crop Sci

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“…The inoculated plants were incubated at 22 8C and photographed at 24 and 48 hpi. The lesion (necrotic) area was quantified using APS Assess: Image Analysis Software for Plant Disease Quantification (APS Press, St. Paul, MN, USA), which was normalized against a square area (1 cm 2 ) photographed in the same image of each leaf (Liang et al, 2013). Italic sequences labelled with 1 and 2 are reverse complemented to the italic sequences labelled with 3 and 4 , respectively.…”

Section: Pathogenicity Assaymentioning

confidence: 99%

Deficiency of the melanin biosynthesis genes SCD1 and THR1 affects sclerotial development and vegetative growth, but not pathogenicity, in Sclerotinia sclerotiorum

Liang

Wang

Steinkellner

et al. 2018

Molecular Plant Pathology

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The fungus Sclerotinia sclerotiorum is a necrotrophic plant pathogen causing significant damage on a broad range of crops. This fungus produces sclerotia that serve as the long-term survival structures in the life cycle and the primary inoculum in the disease cycle. Melanin plays an important role in protecting mycelia and sclerotia from ultraviolet radiation and other adverse environmental conditions. In this study, two genes, SCD1 encoding a scytalone dehydratase and THR1 encoding a trihydroxynaphthalene reductase, were disrupted by target gene replacement, and their roles in mycelial growth, sclerotial development and fungal pathogenicity were investigated. Phylogenetic analyses indicated that the deduced amino acid sequences of SCD1 and THR1 were similar to the orthologues of Botrytis cinerea. Expression of SCD1 was at higher levels in sclerotia relative to mycelia. THR1 was expressed at similar levels in mycelia and sclerotia at early stages, but was up-regulated in sclerotia at the maturation stage. Disruption of SCD1 or THR1 did not change the pathogenicity of the fungus, but resulted in slower radial growth, less biomass, wider angled hyphal branches, impaired sclerotial development and decreased resistance to ultraviolet light.

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“…2014); a polygalacturonase protein that induces calcium signaling and host cell death in soybean (Zuppini et al. 2005); a hypothetical secreted protein that, when disrupted, attenuates virulence in S. sclerotiorum (Liang et al. 2013); a small, cysteine-rich secreted protein with a cyanoviron-N homology (CVNH) domain, which attenuates virulence when deleted (Lyu et al.…”

Section: Introductionmentioning

confidence: 99%

The Complete Genome Sequence of the Phytopathogenic Fungus Sclerotinia sclerotiorum Reveals Insights into the Genome Architecture of Broad Host Range Pathogens

Derbyshire

Denton‐Giles

Hegedus

et al. 2017

Genome Biology and Evolution

186

259

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Sclerotinia sclerotiorum is a phytopathogenic fungus with over 400 hosts including numerous economically important cultivated species. This contrasts many economically destructive pathogens that only exhibit a single or very few hosts. Many plant pathogens exhibit a “two-speed” genome. So described because their genomes contain alternating gene rich, repeat sparse and gene poor, repeat-rich regions. In fungi, the repeat-rich regions may be subjected to a process termed repeat-induced point mutation (RIP). Both repeat activity and RIP are thought to play a significant role in evolution of secreted virulence proteins, termed effectors. We present a complete genome sequence of S. sclerotiorum generated using Single Molecule Real-Time Sequencing technology with highly accurate annotations produced using an extensive RNA sequencing data set. We identified 70 effector candidates and have highlighted their in planta expression profiles. Furthermore, we characterized the genome architecture of S. sclerotiorum in comparison to plant pathogens that exhibit “two-speed” genomes. We show that there is a significant association between positions of secreted proteins and regions with a high RIP index in S. sclerotiorum but we did not detect a correlation between secreted protein proportion and GC content. Neither did we detect a negative correlation between CDS content and secreted protein proportion across the S. sclerotiorum genome. We conclude that S. sclerotiorum exhibits subtle signatures of enhanced mutation of secreted proteins in specific genomic compartments as a result of transposition and RIP activity. However, these signatures are not observable at the whole-genome scale.

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Disruption of a gene encoding a hypothetical secreted protein fromSclerotinia sclerotiorumreduces its virulence on canola (Brassica napus)

Cited by 34 publications

References 20 publications

Identification of new pathogenicity related to the gene encoding hypothetical protein in the gray mold fungus (Botrytis cinerea)

Identification of new pathogenicity related to the gene encoding hypothetical protein in the gray mold fungus (Botrytis cinerea)

Deficiency of the melanin biosynthesis genes SCD1 and THR1 affects sclerotial development and vegetative growth, but not pathogenicity, in Sclerotinia sclerotiorum

The Complete Genome Sequence of the Phytopathogenic Fungus Sclerotinia sclerotiorum Reveals Insights into the Genome Architecture of Broad Host Range Pathogens

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