Disruption of the hypothalamo-pituitary-interrenal axis in 1+ yellow perch (<i>Perca flavescens</i>) chronically exposed to metals in the environment

Gravel, Amélie; Campbell, Peter G. C.; Hontela, Alice

doi:10.1139/f05-011

Cited by 28 publications

(11 citation statements)

References 49 publications

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“…DDT: Benguira et al, 2002), and metals (Gagnon et al, 2006;Gravel et al, 2005;Hontela, 1998;Laflamme et al, 2000;Norris et al, 1999). In particular, effects on the stress axis similar to those observed in the present study have recently been reported for rainbow trout, Oncorhynchus mykiss, exposed to municipal waste water effluent for 14 days (Ings et al, 2011a,b).…”

Section: Consistency Of These Findings With Previous Studiessupporting

confidence: 89%

The stress response of three-spined sticklebacks is modified in proportion to effluent exposure downstream of wastewater treatment works

et al. 2013

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This study was conducted to investigate whether exposure to wastewater treatment works (WWTW) effluent affects the adaptive stress axis of fish resident within the receiving water.Three-spined sticklebacks (Gasterosteus aculeatus) were sampled from sites downstream of ten WWTWs in north-west England, selected to represent a range of human population equivalents between 1,000 and 125,000. Following capture, indices of stress (whole-body cortisol and glucose concentrations) were measured both prior to, and following, the imposition of a standardised stressor to establish both baseline and stress-induced concentrations of cortisol and glucose. There was considerable between-site variation in size, and to a lesser extent condition, of the fish. Pre-and post-stress cortisol and glucose concentrations also varied significantly between-sites. A large proportion of the variation in both the somatic data and the stress response was explained by variation in the proportion of effluent contributing to total river flow at the study sites. Mass (r 2 = 0.35, P < 0.001) and length (r 2 = 0.37, P < 0.001) of the fish, and cortisol (r 2 = 0.26, P < 0.001) and glucose (r 2 = 0.12, P < 0.01) concentrations in unstressed sticklebacks, were positively related to the concentration of effluent across the sample sites. However, in stressed fish, cortisol (r 2 = 0.32, P < 0.001) and glucose (r 2 = 0.14, P < 0.001) concentrations exhibited a negative trend in relation to the effluent concentrations across sites. Individual variation in fish size did not account for the variation in either cortisol or glucose levels. These data provide the first indication that modulation of the stress axis in fish by anthropogenic factors might be widespread and of greater significance than hitherto assumed.

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Section: Consistency Of These Findings With Previous Studiessupporting

confidence: 89%

The stress response of three-spined sticklebacks is modified in proportion to effluent exposure downstream of wastewater treatment works

et al. 2013

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“…Complementary biochemical measurements were carried out on adult (4+), juvenile (1+), and young of the year (YOY) yellow perch (Levesque et al 2002(Levesque et al , 2003Gravel et al 2005). Blood, liver, and muscle samples were collected and analyzed for hormones (cortisol, triiodothyronine T3, thyroxine T4) and various biochemical parameters (glucose, glycogen, lipids).…”

Section: Endocrine and Metabolic Statusmentioning

confidence: 99%

Subcellular Distribution of Cadmium and Nickel in Chronically Exposed Wild Fish: Inferences Regarding Metal Detoxification Strategies and Implications for Setting Water Quality Guidelines for Dissolved Metals

Campbell

Kraemer

Giguère

et al. 2008

Human and Ecological Risk Assessment: An International Journal

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The objective of this study was to investigate metal detoxification in chronically exposed juvenile yellow perch (YP: Perca flavescens) and to field test the commonly assumed threshold toxicity model. Fish were collected from lakes located along a cadmium (Cd) and nickel (Ni) concentration gradient. Ambient dissolved metal concentrations were measured to evaluate exposure and total hepatic metal concentrations were determined as a measure of metal bioaccumulation. Hepatic metal partitioning among potentially metal-sensitive fractions (heat-denatured proteins, organelles) and detoxified metal fractions (metallothionein) was determined after differential centrifugation of YP liver homogenates. Major proportions of hepatic Cd were found in the heat-stable cytosolic peptides and proteins fraction (HSP; including metallothioneins), whereas Ni was mainly found in the potentially metalsensitive heat-denaturable proteins fraction (HDP). For these chronically exposed fish there was no threshold exposure concentration below which binding of Cd or Ni to the heat-denaturable protein fraction or the organelle fraction did not occur. Metal detoxification was clearly incomplete and P. flavescens was subject to some metal-related stress, as evidenced notably by endocrine perturbations. Similar subcellular partitioning results were obtained when juvenile yellow perch were transferred from a reference lake to a Cd-contaminated lake and Cd accumulation was followed over time; there was no accumulation threshold below which Cd binding to the putative metal-sensitive fractions (HDP and organelles) did not occur. The presence of Cd and Ni in these fractions, even for low exposure concentrations and Metal Detoxification in Native Fish low hepatic accumulation, contradicts the threshold toxicity model that underpins metal toxicology theory and that is implicitly used in setting water quality guidelines for metals. Chronically exposed YP appear to have settled for a tradeoff between the cost of turning on their detoxification apparatus at full capacity, to completely suppress metal binding to metal-sensitive sites, and the alternative cost of allowing some binding of inappropriate metals to metal-sensitive sites.

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“…Previous studies examining wild yellow perch inhabiting metal-contaminated lakes have reported impairments in cortisol stress response, intermediary metabolism, seasonal storage of energy reserves and aerobic capacities in the more contaminated fish (Levesque et al 2002;Rajotte and Couture 2002;Couture and Kumar 2003;Gravel et al 2005;Couture et al 2008a). These investigations were carried out in two areas on the Canadian Precambrian Shield that have been subjected to metal emissions from smelters for over 80 years, the Rouyn-Noranda (Quebec) and the Sudbury (Ontario) regions.…”

Section: Introductionmentioning

confidence: 98%

Transcriptional responses to environmental metal exposure in wild yellow perch (Perca flavescens) collected in lakes with differing environmental metal concentrations (Cd, Cu, Ni)

et al. 2009

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To investigate the mechanisms involved in metal stress in wild fish, yellow perch (Perca flavescens) were collected in eight lakes of the Rouyn-Noranda and Sudbury regions (Canada). Due to mining and smelting activities, these two regions indeed present a broad contamination gradient in metal concentrations (Cd, Cu, Zn and Ni; water, sediment and prey) and offer a unique research opportunity to investigate relationships between metal bioaccumulation and resulting deleterious effects in indigenous biota chronically exposed to metal mixtures. The expression level of genes encoding for proteins involved in metal detoxification (metallothioneins, mts), protein protection (heat shock protein-70, hsp-70), growth (insulin-like growth factor-1, igf-1), aerobic energy metabolism (cytochrome c oxydase, cco-1) and protection against oxidative stress (Cu/Zn superoxide dismutase, sod-1) were assessed in fish liver and muscle in association with protein and enzymatic assays for cytochrome c oxidase (CCO). Bioaccumulation of both Cd and Cu increased in response to higher ambient metal concentrations, but the two metals clearly have different modes of action. For Cd, changes in gene expression levels were more marked in the liver than in the dorsal muscle, whereas for Cu the opposite trend was observed. Hepatic Cd accumulation was linked to decreased cco-1 and sod-1 gene expression, whereas Cu accumulation was associated with a decrease in CCO enzymatic activity and an increase in total protein concentration and in cco-1, mts and hsp-70 gene expression levels. For Ni, no significant correlations were observed at the transcriptional level, but increasing hepatic Ni concentrations were significantly and positively correlated with protein concentrations and CCO activity. By coupling gene expression to biochemical and physiological endpoints, this work provides new insights into the mechanisms involved in metal stress and the adaptive response of fish chronically exposed to metal mixtures.

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Disruption of the hypothalamo-pituitary-interrenal axis in 1+ yellow perch (Perca flavescens) chronically exposed to metals in the environment

Cited by 28 publications

References 49 publications

The stress response of three-spined sticklebacks is modified in proportion to effluent exposure downstream of wastewater treatment works

The stress response of three-spined sticklebacks is modified in proportion to effluent exposure downstream of wastewater treatment works

Subcellular Distribution of Cadmium and Nickel in Chronically Exposed Wild Fish: Inferences Regarding Metal Detoxification Strategies and Implications for Setting Water Quality Guidelines for Dissolved Metals

Transcriptional responses to environmental metal exposure in wild yellow perch (Perca flavescens) collected in lakes with differing environmental metal concentrations (Cd, Cu, Ni)

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