Disruption of the quorum sensing regulated pathogenic traits of the biofilm-forming fish pathogen<i>Aeromonas hydrophila</i>by tannic acid, a potent quorum quencher

Patel, Bhakti; Kumari, Supriya; Banerjee, Rajanya; Samanta, Mrinal; Das, Surajit

doi:10.1080/08927014.2017.1336619

Cited by 27 publications

(24 citation statements)

References 41 publications

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“…This information might be useful to design QS inhibitors. The same occurs with tannic acid, a potent quorum quencher that regulates the biofilm formation of A. hydrophila [265].…”

Section: Quorum Sensingmentioning

confidence: 90%

“…The "signal" molecule in Gram-negative bacteria is N-acyl homoserine-lactone (AHL) and in Aeromonas, the AHL is able to modulate the host immune response [19,186,259]. Several studies have shown the importance of AHLs in regulating a range of biological functions such as biofilm formation, antibiotic production, and warming motility and the different QS systems harbor differences in their influences [260][261][262][263][264][265][266]. Chan et al [260] found in the whole genome of the strain A. veronii 159 sequences of QS-related genes, providing a model for exploring it roles in virulence and as a potential target for Aeromonas treatment.…”

Section: Quorum Sensingmentioning

confidence: 99%

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An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity

2020

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The genus Aeromonas belongs to the Aeromonadaceae family and comprises a group of Gram-negative bacteria widely distributed in aquatic environments, with some species able to cause disease in humans, fish, and other aquatic animals. However, bacteria of this genus are isolated from many other habitats, environments, and food products. The taxonomy of this genus is complex when phenotypic identification methods are used because such methods might not correctly identify all the species. On the other hand, molecular methods have proven very reliable, such as using the sequences of concatenated housekeeping genes like gyrB and rpoD or comparing the genomes with the type strains using a genomic index, such as the average nucleotide identity (ANI) or in silico DNA–DNA hybridization (isDDH). So far, 36 species have been described in the genus Aeromonas of which at least 19 are considered emerging pathogens to humans, causing a broad spectrum of infections. Having said that, when classifying 1852 strains that have been reported in various recent clinical cases, 95.4% were identified as only four species: Aeromonas caviae (37.26%), Aeromonas dhakensis (23.49%), Aeromonas veronii (21.54%), and Aeromonas hydrophila (13.07%). Since aeromonads were first associated with human disease, gastroenteritis, bacteremia, and wound infections have dominated. The literature shows that the pathogenic potential of Aeromonas is considered multifactorial and the presence of several virulence factors allows these bacteria to adhere, invade, and destroy the host cells, overcoming the immune host response. Based on current information about the ecology, epidemiology, and pathogenicity of the genus Aeromonas, we should assume that the infections these bacteria produce will remain a great health problem in the future. The ubiquitous distribution of these bacteria and the increasing elderly population, to whom these bacteria are an opportunistic pathogen, will facilitate this problem. In addition, using data from outbreak studies, it has been recognized that in cases of diarrhea, the infective dose of Aeromonas is relatively low. These poorly known bacteria should therefore be considered similarly as enteropathogens like Salmonella and Campylobacter.

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“…This information might be useful to design QS inhibitors. The same occurs with tannic acid, a potent quorum quencher that regulates the biofilm formation of A. hydrophila [265].…”

Section: Quorum Sensingmentioning

confidence: 90%

Section: Quorum Sensingmentioning

confidence: 99%

An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity

2020

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“…This study also confirmed that A. veronii isolated from the intestinal tract of fish was different from that isolated from the aquatic environment. Aerolysin is the main virulent factor in Aeromonas, contributing to bacterial pathogenesis [33,34]. However, in the 1990s, the aerolysin gene had not been detected in any A. veronii isolates [35].…”

Section: Discussionmentioning

confidence: 99%

Aeromonas veronii Infection in Commercial Freshwater Fish: A Potential Threat to Public Health

Li¹,

Raza

Yang³

et al. 2020

Animals

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Aeromonas veronii is an important pathogen causing freshwater fish sepsis and ulcer syndrome. An increasing number of cases have demonstrated its significance as an aquatic zoonotic agent. The purpose of this study was to ensure the safety of freshwater products by evaluating the infection status of edible freshwater fish. In this experiment, we isolated A. veronii from several species of apparently healthy freshwater fish, including Carassius auratus, Cyprinus carpio, Ctenopharyngodon idella, and Silurus asotus. A. veronii was identified through bacterial staining, culture characteristics, and 16S rDNA gene sequence. In addition, polymerase chain reaction (PCR) was used to investigate the distribution of seven major virulence genes, including aerolysin (aer: 88.51%), cytotoxic enterotoxin (act: 71.26%), serine proteinase (ser: 54.02%), adhesin (Aha: 40.23%), phospholipase (lip: 45.98%), nuclease (exu: 51.72%), and quorum sensing-controlled virulence factor (LuxS: 59.77%). In total, 496 strains of Aeromonas were isolated, including 87 strains of A. veronii. The isolates of A. veronii were Gram-negative, rod-shaped bacteria, and the colonies are yellow on Rimler-Shotts (RS) medium and showed greater than 99% homology with A. veronii ATCC35624 according to analyses of the 16S rDNA sequence. Nearly 50% of the A. veronii isolates carried at least four or more virulence genes, 25% of the isolates carried at least five types of virulence genes, and 59.77% isolates carried the LuxS gene, and the isolates carrying more virulence genes were found to be more virulent. These results are of great significance for further improving the food safety assessment of freshwater aquatic products.

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“…Turmeric also has been reported able to inhibit virulence genes expression on P. aeruginosa [18]. Tannic acid-treated A. hydrophila showed a considerable reduction in violacein production, blood hemolysis activity, the pattern of swarming motility and biofilm formation [16]. Quorum sensing have been known regulates expression of various virulence factor genes from pathogenic bacteria.…”

Section: Discussionmentioning

confidence: 99%

Quorum Sensing Inhibition Activity of Water Extract of Rhizome Herbs onAeromonas hydrophila

Triyitno

Murwantoko

2020

E3S Web Conf.

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Several bacteria have been reported to be pathogenic to fishes, and some of them were opportunistic pathogen bacteria. Quorum sensing is a widespread phenomenon in bacteria on coordinating gene expression of virulence factors of bacterial pathogens, including biofilms. The aims of this research were to confirm biofilm formation by fish pathogenic bacteria, determine activity of water extracts of medicinal rhizome herb on growth and quorum sensing inhibition of Aeromonas hydrophila. Turmeric (Curcuma longa L.), temu lawak (Curcuma xanthoriza Roxb), ginger (Zingiber officinale Roscoe), kencur (Kaempferia galanga L.) and galangal (Alpinia galangal L.) rhizomes were used in this study. The extracts were examined on their activity to affect growth and inhibition of biofilms formation by A. hydrophila at concentrations of 40, 30, 20 and 10 mg/ml. Extract of rhizome medicinal herbs did not affect the growth of A. hydrophila. Ginger extract at concentration of 40 mg /ml showed the highest inhibition of biofilm formation by 79.7%. Turmeric extract inhibited the biofilm formation by 59.9% at concentration of 30 mg / ml. Kencur extract showed the lowest biofilm inhibitory ability of 7.0%. The results showed the addition of plant rhizome water extracts had anti-quorum sensing activity as evidenced by inhibition of the formation of these bacterial biofilms.

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Disruption of the quorum sensing regulated pathogenic traits of the biofilm-forming fish pathogenAeromonas hydrophilaby tannic acid, a potent quorum quencher

Cited by 27 publications

References 41 publications

An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity

An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity

Aeromonas veronii Infection in Commercial Freshwater Fish: A Potential Threat to Public Health

Quorum Sensing Inhibition Activity of Water Extract of Rhizome Herbs onAeromonas hydrophila

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