Dissecting the Interaction Deficiency of a Cartilaginous Fish Digestive Lipase with Pancreatic Colipase: Biochemical and Structural Insights

Achouri, Neila; Tomàs-Gamisans, Màrius; Triki-Fendri, Soumaya; Valero, Francisco; Miled, Nabil; Fendri, Ahmed; Smichi, Nabil

doi:10.1155/2020/3064290

Cited by 3 publications

(3 citation statements)

References 43 publications

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“…Genetic and environmental manipulations can be conducted more effectively on bacterial cells to improve cell yields and enzymatic processes, or to generate altered enzymes, owing to their brief generation cycles, simple nutritional requirements, and simple screening procedures for desired properties (Achouri et al, 2020). Bacillus sp.…”

Section: International Journal Of Zoological Investigations Issn: 245...mentioning

confidence: 99%

16S rRNA Sequencing of Lipase Producing Bacterial Strain Isolated from Fish

Deepa¹,

Anuanandhi²,

Sankar³

et al. 2021

IJZI

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Section: International Journal Of Zoological Investigations Issn: 245...mentioning

confidence: 99%

16S rRNA Sequencing of Lipase Producing Bacterial Strain Isolated from Fish

Deepa¹,

Anuanandhi²,

Sankar³

et al. 2021

IJZI

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“…Here we focused on the two types of digestive lipase (E.C. 3.1.1.3) genes, pancreatic lipase gene ( pl ) and bile salt-activated lipase gene ( bsal ), they mainly catalyze the hydrolysis of triacylglycerols into glycerol and fatty acids ( Sæle et al, 2010 ; Holmes and Cox, 2011 ; Achouri et al, 2020 ; Qiu et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, pancreatic lipase is a colipase-dependent lipase, colipase is required for lipid binding and allows the pancreatic lipase to carry out the function of hydrolyzing lipids (Smichi et al, 2017;Anderson et al, 2018;González-Félix ML. et al, 2018;Achouri et al, 2020). The absence of the colipase gene (clps) also can be found in zebrafish (Danio rerio), medaka (Oryzias latipe), Japanese pufferfish (Takifugu rubripes), and three-spined stickleback (Gasterosteus aculeatus) (Saele et al, 2010;Smichi et al, 2017;Anderson et al, 2018;Saele et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of the Molecular Characterization, Evolution, and Structure Modeling of Digestive Lipase Genes Reveals the Different Evolutionary Selection Between Mammals and Fishes

Tang

Liang

et al. 2022

Front. Genet.

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Vertebrates need suitable lipases to digest lipids for the requirement of energy and essential nutrients; however, the main digestive lipase genes of fishes have certain controversies. In this study, two types of digestive lipase genes (pancreatic lipase (pl) and bile salt-activated lipase (bsal)) were identified in mammals and fishes. The neighborhood genes and key active sites of the two lipase genes were conserved in mammals and fishes. Three copies of PL genes were found in mammals, but only one copy of the pl gene was found in most of the fish species, and the pl gene was even completely absent in some fish species (e.g., zebrafish, medaka, and common carp). Additionally, the hydrophobic amino acid residues (Ile and Leu) which are important to pancreatic lipase activity were also absent in most of the fish species. The PL was the main digestive lipase gene in mammals, but the pl gene seemed not to be the main digestive lipase gene in fish due to the absence of the pl gene sequence and the important amino acid residues. In contrast, the bsal gene existed in all fish species, even two to five copies of bsal genes were found in most of the fishes, but only one copy of the BSAL gene was found in mammals. The amino acid residues of bile salt-binding sites and the three-dimensional (3D) structure modeling of Bsal proteins were conserved in most of the fish species, so bsal might be the main digestive lipase gene in fish. The phylogenetic analysis also indicated that pl or bsal showed an independent evolution between mammals and fishes. Therefore, we inferred that the evolutionary selection of the main digestive lipase genes diverged into two types between mammals and fishes. These findings will provide valuable evidence for the study of lipid digestion in fish.

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Intestinal Lipase Characterization in Common Snook (Centropomus undecimalis) Juveniles

Concha‐Frías¹,

Gaxiola²,

Cruz-Alvarado

et al. 2022

Fishes

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The common snook (Centropomus undecimalis) is a euryhaline fish with high commercial demand in the Mexican southeast, Caribbean, and South America. However, some aspects of its digestive physiology are still unknown, particularly in relation to lipid hydrolysis. Therefore, the characterization of the digestive lipase of this species was carried out. Our results show that the digestive lipase’s optimal temperature is 35 °C, being stable between 25 and 35 °C, and shows maximum activity at pH 9, with stability between pH 5 and 8. Different degrees of inhibition were presented by Orlistat (61.4%), Ebelactone A (90.36%), Ebelactone B (75.9%), SDS 1% (80.7%), SDS 0.1% (73.5%), and SDS at 0.01% (34.9%). Orlistat and Ebelactone A and B completely inhibited the lipase band in the zymogram, but not SDS addition. Lipase showed a molecular weight of 43.8 kDa. The high lipase activities in the digestive tract indicate the importance of lipids in the diet of C. undecimalis.

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Dissecting the Interaction Deficiency of a Cartilaginous Fish Digestive Lipase with Pancreatic Colipase: Biochemical and Structural Insights

Cited by 3 publications

References 43 publications

16S rRNA Sequencing of Lipase Producing Bacterial Strain Isolated from Fish

16S rRNA Sequencing of Lipase Producing Bacterial Strain Isolated from Fish

Comparative Study of the Molecular Characterization, Evolution, and Structure Modeling of Digestive Lipase Genes Reveals the Different Evolutionary Selection Between Mammals and Fishes

Intestinal Lipase Characterization in Common Snook (Centropomus undecimalis) Juveniles

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