Dissecting the structure–function relationship in lysozyme domain of mycobacteriophage D29‐encoded peptidoglycan hydrolase

Joshi, Himanshu; Seniya, Surya Pratap; Venkatesan, S.; Patidar, Neelam Devidas; Jain, Vikas

doi:10.1002/1873-3468.12848

Cited by 13 publications

(7 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, 71.7% of the prophages have genes encoding lysozymes and 54.7% have genes encoding holins. Lysozymes are peptidoglycan hydrolases and holins cause plasmatic membrane damage, the action of these enzymes leading to cell disruption 11 , 32 . The presence of these enzymes characterizes these phages as infective, highlighting an attractive feature for their isolation by induction and infection assays with different strains, as seen by Walker et al .…”

Section: Discussionmentioning

confidence: 99%

Screening and characterization of prophages in Desulfovibrio genomes

Crispim

Dias

Vidigal

et al. 2018

Sci Rep

View full text Add to dashboard Cite

Bacteria of the genus Desulfovibrio belong to the group of Sulphate Reducing Bacteria (SRB). SRB generate significant liabilities in the petroleum industry, mainly due to their ability to microbiologically induce corrosion, biofilm formation and H2S production. Bacteriophages are an alternative control method for SRB, whose information for this group of bacteria however, is scarce. The present study developed a workflow for the identification of complete prophages in Desulfovibrio. Poly-lysogenesis was shown to be common in Desulfovibrio. In the 47 genomes analyzed 53 complete prophages were identified. These were classified within the order Caudovirales, with 69.82% belonging to the Myoviridade family. More than half the prophages identified have genes coding for lysozyme or holin. Four of the analyzed bacterial genomes present prophages with identity above 50% in the same strain, whose comparative analysis demonstrated the existence of colinearity between the sequences. Of the 17 closed bacterial genomes analyzed, 6 have the CRISPR-Cas system classified as inactive. The identification of bacterial poly-lysogeny, the proximity between the complete prophages and the possible inactivity of the CRISPR-Cas in closed bacterial genomes analyzed allowed the choice of poly-lysogenic strains with prophages belonging to the Myoviridae family for the isolation of prophages and testing of related strains for subsequent studies.

show abstract

Section: Discussionmentioning

confidence: 99%

Screening and characterization of prophages in Desulfovibrio genomes

Crispim

Dias

Vidigal

et al. 2018

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The ability of mycobacteriophage D29 to quickly penetrate and eradicate pathogens is the reason why researchers have considered it as a new therapeutic option against MDR and XDR bacteria, especially Mtb 53. This mycobacteriophage is used for several targets, particularly in TB diagnosis and probably in TB treatment 54.…”

Section: Phage Therapy In Tuberculosismentioning

confidence: 99%

“…Lysin A coded by gp10 hydrolyzes peptidoglycan of bacterial cell walls 57. Gp12 encodes Lysin B that leads to the dissemination of mycolic acid from the peptidoglycans in mycobacterial cell wall 53,58. Holin as a membrane pore-forming protein participates in the transfer of lysins from the cytosol to the periplasm 59.…”

Section: Phage Therapy In Tuberculosismentioning

confidence: 99%

<p>Phage therapy as a renewed therapeutic approach to mycobacterial infections: a comprehensive review</p>

Azimi¹,

Mosadegh²,

Nasiri³

et al. 2019

IDR

View full text Add to dashboard Cite

Mycobacterial infections are considered to a serious challenge of medicine, and the emergence of MDR and XDR tuberculosis is a serious public health problem. Tuberculosis can cause high morbidity and mortality around the world, particularly in developing countries. The emergence of drug-resistant Mycobacterium infection following limited therapeutic technologies coupled with the serious worldwide tuberculosis epidemic has adversely affected control programs, thus necessitating the study of the role bacteriophages in the treatment of mycobacterial infection. Bacteriophages are viruses that are isolated from several ecological specimens and do not exert adverse effects on patients. Phage therapy can be considered as a significant alternative to antibiotics for treating MDR and XDR mycobacterial infections. The useful ability of bacteriophages to kill Mycobacterium spp has been explored by numerous research studies that have attempted to investigate the phage therapy as a novel therapeutic/diagnosis approach to mycobacterial infections. However, there are restricted data about phage therapy for treating mycobacterial infections. This review presents comprehensive data about phage therapy in the treatment of mycobacterial infection, specifically tuberculosis disease.

show abstract

“…In another study, the same authors proposed that the D29 LysA interdomain interaction occurs intramolecularly and is facilitated by a charged linker that connects the two domains [ 27 ]. In addition, the central catalytic domain that has been predicted to possess glycoside hydrolase (GH19) activity was shown to possess a residue R198, positioned away from the catalytic site and not directly involved in substrate binding, which plays a critical role in the maintenance of the substrate-binding cavity [ 76 ]. Taken together these data indicate that mycobacteriophage endolysin activity regulation may be more complex than previously anticipated, involving intramolecular domains interaction and synthesis of secondary gene products in order to optimize mycobacteria CW binding and enzyme activation; these mechanisms may improve PG breakdown which will culminate in optimal bacterial cell rupture and phage release.…”

Section: Mycobacteriophages Endolysin (Lysa)—the Essential Lysis Pmentioning

confidence: 99%

Mycobacteriophage Lysis Enzymes: Targeting the Mycobacterial Cell Envelope

Catalão

Pimentel

2018

Viruses

View full text Add to dashboard Cite

Mycobacteriophages are viruses that specifically infect mycobacteria, which ultimately culminate in host cell death. Dedicated enzymes targeting the complex mycobacterial cell envelope arrangement have been identified in mycobacteriophage genomes, thus being potential candidates as antibacterial agents. These comprise lipolytic enzymes that target the mycolic acid-containing outer membrane and peptidoglycan hydrolases responsive to the atypical mycobacterial peptidoglycan layer. In the recent years, a remarkable progress has been made, particularly on the comprehension of the mechanisms of bacteriophage lysis proteins activity and regulation. Notwithstanding, information about mycobacteriophages lysis strategies is limited and is mainly represented by the studies performed with mycobacteriophage Ms6. Since mycobacteriophages target a specific group of bacteria, which include Mycobacterium tuberculosis responsible for one of the leading causes of death worldwide, exploitation of the use of these lytic enzymes demands a special attention, as they may be an alternative to tackle multidrug resistant tuberculosis. This review focuses on the current knowledge of the function of lysis proteins encoded by mycobacteriophages and their potential applications, which may contribute to increasing the effectiveness of antimycobacterial therapy.

show abstract

Dissecting the structure–function relationship in lysozyme domain of mycobacteriophage D29‐encoded peptidoglycan hydrolase

Cited by 13 publications

References 35 publications

Screening and characterization of prophages in Desulfovibrio genomes

Screening and characterization of prophages in Desulfovibrio genomes

<p>Phage therapy as a renewed therapeutic approach to mycobacterial infections: a comprehensive review</p>

Mycobacteriophage Lysis Enzymes: Targeting the Mycobacterial Cell Envelope

Contact Info

Product

Resources

About