Dissection of Antigen-Specific Humoral and Cellular Immune Responses for the Development of Experimental Immune-Mediated Blepharoconjunctivitis in C57BL/6 Mice

Fukushima, Atsuki; Ozaki, Akemi; Zhang, Jian; Ishida, Waka; Fukata, Kazuyo; Ueno, Hideki; Liu, Fu‐Tong

doi:10.1080/02713680590927560

Cited by 43 publications

(51 citation statements)

References 14 publications

(10 reference statements)

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“…Similar to data obtained for RW-induced EC, [5][6][7][8][9][10] we observed both EPR and LPR in Cry j-induced EC. Further- Fig.…”

Section: Resultssupporting

confidence: 79%

“…In animal models of AC, short ragweed pollen (RW) is most common antigen used. [5][6][7][8][9][10] However, Cry j-induced AC is far more common in Japan than RW-induced AC. 11) Since different allergens may induce different immune response characteristics, 12) investigations using Cry j-induced conjunctivitis can provide information that is relevant to Japanese pollinosis patients.…”

mentioning

confidence: 99%

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Cryptomeria japonica-Induced Allergic Conjunctivitis in Mice

Fukushima

Shii

Sumi

et al. 2007

Biological & Pharmaceutical Bulletin

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Japanese cedar pollen (Cryptomeria japonica, Cry j) is the most common allergen causing pollinosis in Japan. However, short ragweed pollen is used commonly as the antigen for experimentally-induced allergic conjunctivitis (EC) and Cry j-induced EC in mice has not been published. We actively immunized BALB/c mice with Cry j, and then performed a challenge with eye drops containing Cry j. We evaluated the early phase and late phase reactions in the conjunctiva, using Evans blue dye leakage and eosinophil infiltration, respectively. Significant inhibition of the early phase reaction was observed following pre-challenge with eye drops that block histamine H1 receptor in the conjunctiva. Thus, Cry j-induced EC appears to represent a suitable model for the study of pollinosis in Japan.

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“…Similar to data obtained for RW-induced EC, [5][6][7][8][9][10] we observed both EPR and LPR in Cry j-induced EC. Further- Fig.…”

Section: Resultssupporting

confidence: 79%

mentioning

confidence: 99%

Cryptomeria japonica-Induced Allergic Conjunctivitis in Mice

Fukushima

Shii

Sumi

et al. 2007

Biological & Pharmaceutical Bulletin

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“…Supporting this is that Th2-type cytokines are detected in tears of most patients with severe AC (7,8). To understand how Th2 cells are involved in AC development, our group has established an experimental model of AC in rats (9,10) and mice (11,12) denoted as experimental immune-mediated blepharoconjunctivitis (EC). This model involves injecting the animals with an allergen, followed by challenge with allergen-containing eye drops.…”

Section: A Llergic Conjunctivitis (Ac)mentioning

confidence: 99%

“…Alternatively, the animals are passively primed by adoptive transfer of splenocytes or T cells from allergen-primed animals or allergenspecific IgE. In mice, the active sensitization method induces prominent Th2 immune responses and abundant eosinophilic infiltration into the conjunctiva, especially in Th2-prone BALB/c mice (11). The passive sensitization of naive BALB/c mice by allergen-primed T cells or allergen-specific IgE also induces severe eosinophilic infiltration (13).…”

Section: A Llergic Conjunctivitis (Ac)mentioning

confidence: 99%

Engagement of 4-1BB Inhibits the Development of Experimental Allergic Conjunctivitis in Mice

Fukushima

Yamaguchi

Ishida

et al. 2005

The Journal of Immunology

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The 4-1BB receptor acts as a costimulator in CD8+ T cell activation. Agonistic stimulation through this molecule by treatment with anti-4-1BB Abs has been demonstrated to inhibit various experimentally induced diseases in animals. However, the effect of anti-4-1BB Abs on experimental allergic diseases has not been reported. We investigated the effect of anti-4-1BB Abs on the development and progression of experimental allergic conjunctivitis in mice. To examine the effects of Abs during the induction or effector phase, actively immunized mice or passively immunized mice by splenocyte transfer were treated with agonistic anti-4-1BB Abs, blocking anti-4-1BB ligand Abs, or normal rat IgG. Eosinophil infiltration into the conjunctiva was significantly reduced in wild-type mice by the anti-4-1BB Ab treatment during either induction or effector phase. Th2 cytokine production by splenocytes and total serum IgE were significantly reduced by the anti-4-1BB Ab treatment, while IFN-γ production was increased. The anti-4-1BB Ab treatment induced a relative increase of CD8-positive cell numbers in the spleens. Moreover, inhibition of eosinophil infiltration by the treatment with anti-4-1BB Abs was also noted in actively immunized IFN-γ knockout mice. Taken altogether, in vivo treatment with agonistic anti-4-1BB Abs in either induction or effector phase inhibits the development of experimental allergic conjunctivitis, and this inhibition is likely to be mediated by suppression of Th2 immune responses rather than up-regulation of IFN-γ.

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“…Once initiated, LPR can proceed in the presence of little or no detectable allergen-specific IgE antibody. LPR can also be induced by adoptively transference of T cells from allergensensitized donors to naïve recipients prior to challenging the ocular surface with the specific antigen (Fukushima et al, 2005). LPR could lead to corneal complications secondary to eosinophil infiltration.…”

Section: Effector Phase Reactionmentioning

confidence: 99%