Dissection of barley chromosome 5H in common wheat

Abstract

We dissected barley chromosome 5H added to common wheat by a genetic method or the gametocidal system. Firstly, we induced chromosomal breaks in the offspring of a 5H addition line of common wheat carrying a gametocidal chromosome and cytologically screened for plants with structural chromosomal changes involving 5H, such as deletions and translocations. Secondly, we screened the progeny of such plants to establish common wheat lines carrying structurally changed chromosomes containing single segments of the dissected 5H. Using 23 representative 5H dissection lines, we physically mapped 97 barley EST markers assigned to 5H. The ESTs fell into 20 regions of 5H between the breakpoints of the 23 dissected segments, distributing rather evenly along the chromosome, with significantly higher frequency in the distal region of the long arm. The ESTs, in turn, allowed us to distinguish the breakpoints of dissected 5H segments. We demonstrated by PCR (polymerase chain reaction), as well as by in situ hybridization, that these dissected 5H segments were stably transmitted in the dissection lines. We discuss the usefulness of the 5H dissection lines for physical mapping of DNA markers. These 5H dissection lines are available from National BioResource Projects-Wheat, Japan.

Wheat–barley hybridization: the last 40 years

“…These 7H deletion and translocation lines were then used for the physical mapping of the 7H barley chromosome (Serizawa et al 2001). Nasuda et al (2005), Ashida et al (2007) and Sakai et al (2009) performed deletion mapping on barley chromosomes 7H, 5H and 3H using barley dissection lines and barley-specific EST Euphytica (2014) 195:315-329 321 markers. The barley dissection lines were produced from CS-Betzes addition lines, so they all carried chromosome segments from Betzes barley.…”

A high-density transcript linkage map of barley derived from a single population

“…For example, Nasuda et al (2005) and Ashida et al (2007) mapped barley EST markers on the barley chromosome deletion stocks and estimated the physical location of these ESTs. Although the resolution in segmental allocation of markers was low in wheat deletion stock mapping (Sorrells et al, 2003), the approach is a useful first step in developing BAC contigs for genome sequencing.…”

Cytological dissection of barley genome by the gametocidal system

“…Endo (1999, 2000) demonstrated that chromosome 2C is capable of inducing structural changes in every barley chromosome in 'Chinese Spring'. Shi and Endo (2000), Ashida et al (2007) and Sakai et al (2008) employed the Gc system with chromosome 2C or 3C SAT to induce structural changes in barley chromosomes 3H, 5H and 7H, and establish so-called 'barley dissection lines' of common wheat. These works showed that chromosome 3C SAT is as effective as chromosome 2C in inducing structural rearrangements in barley chromosomes.…”

“…Serizawa et al (2001) conducted deletion mapping of AFLP and STS markers that had been used in a genetic mapping and translocation mapping (Künzel et al 2000) and found that the marker orders matched previous maps. Using the barley dissection lines and barley-specific EST markers (Nasuda et al 2005a), Nasuda et al (2005a), Ashida et al (2007) and Sakai et al (2009) conducted deletion mapping of barley chromosomes 7H, 5H and 3H, respectively. All these studies revealed that the distal regions of the barley chromosomes are richer in EST markers than the proximal regions.…”