Dissection of Oxidative Stress Tolerance Using Transgenic Plants

Allen, Randy D.

doi:10.1104/pp.107.4.1049

Cited by 639 publications

(348 citation statements)

References 15 publications

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“…It is vital for plants to adjust the enzymatic and nonenzymatic antioxidant systems to control the amounts of ROS to avoid oxidative stress (Allen 1995). The regulation of the activities of SOD, CAT and ascorbate-glutathione cycle enzymes is a rapid and efficient response to limit the excess of ROS generated by environmental stresses and it was observed in different fruits (Scebba et al 2001;Sofo et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Effect of water stress on antioxidant systems and oxidative parameters in fruits of tomato (Solanum lycopersicon L, cv. Micro-tom)

Murshed

Lopez-Lauri²,

Sallanon

2013

Physiol Mol Biol Plants

100

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The effects of different levels of water stress on oxidative parameters (H 2 O 2 and MDA), the total pool sizes of ascorbate, the activities of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), as well as the activities and relative transcript levels of the enzymes of ascorbate-glutathione cycle ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR) and glutathione reductase (GR) were studied in the fruit of tomato (Solanum lycopersicum L. cv. Micro-Tom). Plants were subjected to three levels of water stress (S50, S25 and S0) and fruits at different development stages were harvested after 3, 6 and 10 days of stress. Changes in H 2 O 2 and MDA contents indicated that water stress induced oxidative stress in fruits. The concentrations of ascorbate (AsA) and dehydroascorbate (DHA) generally modified with water stress treatments. Moreover, changes in SOD and CAT activities and DHAR, MDHAR, APX and GR activities and relative transcript levels were dependent on the fruit development stage and the intensity and the duration of water stress. These results suggest that the response of antioxidant systems of tomato fruits to oxidative stress induced by water stress treatments was different depending on the fruit development stage.

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Section: Discussionmentioning

confidence: 99%

Effect of water stress on antioxidant systems and oxidative parameters in fruits of tomato (Solanum lycopersicon L, cv. Micro-tom)

Murshed

Lopez-Lauri²,

Sallanon

2013

Physiol Mol Biol Plants

100

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“…The generation of ROS could be indicated by an increase in the amount of MDA concentration and electrolytic leakage in the present study, which supports our hypothesis that high light increases the breakdown of lipid membrane. Tolerance to light stress is generally accompanied by an increase in levels of antioxidants, and the activities of antioxidant enzymes (Allen 1995), protecting the photosystem from ROS. T. indica demonstrated diverse responses to antioxidant enzymes indicating that oxidative stress is an influential component of light stress.…”

Section: Discussionmentioning

confidence: 99%

Effect of light irradiations on photosynthetic machinery and antioxidative enzymes duringex vitroacclimatization ofTylophora indicaplantlets

Faisal

Anis

2010

Journal of Plant Interactions

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In the present communication, we studied the effect of light stress on pigment contents, net photosynthetic rate, electrolyte leakage, malondialdehyde concentration and various antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), and ascorbate peroxidase (APX) during acclimatization of micropropagated Tylophora indica plantlets. Pigment (Chlorophyll a, b and carotenoids) contents in ex vitro formed leaves were found significantly higher compared to the in vitro formed ones. In vitro plantlets (day 0) exhibited a low photosynthetic activity, but with the emergence of new leaves a significant increase in net photosynthetic rates was observed. Changes in the activity of the antioxidant enzymes system were also observed during the critical days of acclimatization. Plantlets showed increased levels of SOD production, indicating its preventive mechanism of membrane oxidation and damage to biological molecules in high light (HL) acclimatized plantlets. The CAT activity increased at both low lights (LL) and HL during the whole period of acclimatization. Likewise, photoexposure of plantlets at LL and HL showed elevated activity of GR and APX against 0 day plantlets.

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“…Inevitably, therefore, ROS scavenging capacity has been long recognised as a target for genetic engineering (Foyer et al 1994;Allen 1995;Bhatnagar-Mathur et al 2008), and over-expression of genes encoding enzymic components of these defences has been explored in a number of studies. The most intensively studied has been the over-expression of SOD.…”

Section: Introductionmentioning

confidence: 99%

Modification of reactive oxygen species scavenging capacity of chloroplasts through plastid transformation

et al. 2011

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Reactive oxygen species (ROS), including superoxide anions, hydrogen peroxide and hydroxyl radicals are generated through normal biochemical processes, but their production is increased by abiotic stresses. The prospects for enhancing ROS scavenging, and hence stress tolerance, by direct gene expression in a vulnerable cell compartment, the chloroplast, have been explored in tobacco. Several plastid transformants were generated which contained either a Nicotiana mitochondrial superoxide dismutase (MnSOD) or an Escherichia coli glutathione reductase (gor) gene. MnSOD lines had a three-fold increase in MnSOD activity, but interestingly a five to nine-fold increase in total chloroplast SOD activity. Gor transgenic lines had up to 6 times higher GR activity and up to 8 times total glutathione levels compared to wild type tobacco. Photosynthetic capacity of transplastomic plants, as measured by chlorophyll content and variable fluorescence of PSII was equivalent to non-transformed plants.The response of these transplastomic lines to several applied stresses was examined. In a number of cases improved stress tolerance was observed. Examples include enhanced methyl viologen (Paraquat)-induced oxidative stress tolerance in Mn-superoxidase dismutase overexpressing plants, improved heavy metal tolerance in glutathione reductase expressing lines, and improved tolerance to UV-B radiation in both sets of plants.

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Dissection of Oxidative Stress Tolerance Using Transgenic Plants

Cited by 639 publications

References 15 publications

Effect of water stress on antioxidant systems and oxidative parameters in fruits of tomato (Solanum lycopersicon L, cv. Micro-tom)

Effect of water stress on antioxidant systems and oxidative parameters in fruits of tomato (Solanum lycopersicon L, cv. Micro-tom)

Effect of light irradiations on photosynthetic machinery and antioxidative enzymes duringex vitroacclimatization ofTylophora indicaplantlets

Modification of reactive oxygen species scavenging capacity of chloroplasts through plastid transformation

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