Dissection of the genus Actinobaculum: Reclassification of Actinobaculum schaalii Lawson et al. 1997 and Actinobaculum urinale Hall et al. 2003 as Actinotignum schaalii gen. nov., comb. nov. and Actinotignum urinale comb. nov., description of Actinotignum sanguinis sp. nov. and emended descriptions of the genus Actinobaculum and Actinobaculum suis; and re-examination of the culture deposited as Actinobaculum massiliense CCUG 47753T ( = DSM 19118T), revealing that it does not represent a strain of this spec

Abstract

The remarkable host specificity of the species of the genus Actinobaculum led us to recharacterize these species by a polyphasic approach. A comparative chemotaxonomic study including analysis of whole-cell sugars, amino acid composition of the peptidoglycan, fatty acid methyl esters, respiratory quinones and polar lipids revealed significant differences that, in combination with molecular data, support a dissection of the genus Actinobaculum. The proposals of this study include the reclassification of Actinobaculum schaalii and Actinobaculum urinale as Actinotignum schaalii gen. nov., comb. nov. (type strain DSM 15541 T 5CCUG 27420 T) and Actinotignum urinale comb. nov. (type strain DSM 15805 T 5CCUG 46093 T), respectively. Emended descriptions of the genus Actinobaculum and Actinomyces suis are also provided. The results of 16S rRNA gene sequence analysis and DNA-DNA hybridization also indicated that the type strain of Actinobaculum massiliense deposited as CCUG 47753 T (5DSM 19118 T) should in fact be considered a member of the species Actinobaculum schaalii. In addition, comparative 16S rRNA gene sequencing and DNA-DNA relatedness studies of four strains recovered from clinical materials demonstrated that three of the isolates belonged to Actinotignum schaalii; the remaining strain represents a novel species, for which the name Actinotignum sanguinis sp. nov. is proposed. The type strain is IMMIB L-2199 T (5DSM 26039 T 5CCUG 64068 T). Abbreviation: DDH, DNA-DNA hybridization. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain IMMIB L-2199 T is HG798952. Two supplementary tables and two supplementary figures are available with the online Supplementary Material.

Propionimicrobium lymphophilum and Actinotignum schaalii bacteraemia: a case report

“…As of 2015, the genus Actinobaculum was divided into Actinotignum and Actinobaculum [6]. A. schaalii is currently considered to be an emerging uropathogen because it has been associated with urinary tract infections (UTIs) in many cases [7], [8], [9], [10].…”

Whole-Genome Sequences of Two Closely Related Bacteria, Actinomyces sp. Strain Chiba101 and Actinomyces denticolens DSM 20671 T

“…These isolates have been found to be pathogenic in pigs (2, 3). However, since no type strain of A. suis Franke 1973 was extant (4), it is impossible to compare strain Chiba101 and A. suis Franke 1973. On the other hand, Actinomyces denticolens has been known as a commensal bacterium found in the oral cavity of cattle (5).…”

Ancrocorticia populi gen. nov., sp. nov, isolated from the symptomatic bark of Populus × euramericana canker

“…Following several revisions, membership of the family was restricted to bacterial species. Recently, the application of modern taxonomic techniques such as chemotaxonomics, numerical phonetics, and molecular genetics to the respective organisms has resulted in inclusion of the genera Actinobaculum , Actinomyces , Arcanobacterium , Flaviflexus , Mobiluncus , Trueperella , Varibaculum , and Actinotignum in the Actinomycetaceae (Schaal, Yassin, & Stackebr, ; Yassin et al, ).…”