Dissection of Trichoderma longibrachiatum-induced defense in onion (Allium cepa L.) against Fusarium oxysporum f. sp. cepa by target metabolite profiling

Abstract

Trichoderma spp. are versatile opportunistic plant symbionts that can cause substantial changes in the metabolism of host plants, thereby increasing plant growth and activating plant defense to various diseases. Target metabolite profiling approach was selected to demonstrate that Trichoderma longibrachiatum isolated from desert soil can confer beneficial agronomic traits to onion and induce defense mechanism against Fusarium oxysporum f. sp. cepa (FOC), through triggering a number of primary and secondary metabolite pathways. Onion seeds primed with Trichoderma T1 strain displayed early seedling emergence and enhanced growth compared with Trichoderma T2-treatment and untreated control. Therefore, T1 was selected for further investigations under greenhouse conditions, which revealed remarkable improvement in the onion bulb growth parameters and resistance against FOC. The metabolite platform of T1-primed onion (T1) and T1-primed onion challenged with FOC (T1+FOC) displayed significant accumulation of 25 abiotic and biotic stress-responsive metabolites, representing carbohydrate, phenylpropanoid and sulfur assimilation metabolic pathways. In addition, T1- and T1+FOC-treated onion plants showed discrete antioxidant capacity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) compared with control. Our findings demonstrated the contribution of T. longibrachiatum to the accumulation of key metabolites, which subsequently leads to the improvement of onion growth, as well as its resistance to oxidative stress and FOC.

Different mechanisms of Trichoderma virens‐mediated resistance in tomato against Fusarium wilt involve the jasmonic and salicylic acid pathways

“…Likewise, T . longibrachiatum treatment of bulb onions improved growth parameters such as seedling height and FW (Abdelrahman et al ., ). The enhanced seed germination rates and plant growth parameters observed in this study might be caused by successful colonization by TriV_JSB100, leading to an improvement in nutrient uptake and induction of indole acetic acid (Abdelrahman et al ., ; Jogaiah et al ., ; Shoresh et al ., ; Yadav et al ., ).…”

“…). A similar correlation was observed in our previous research (Abdelrahman et al ., ), where the treatment of onion ( Allium cepa ) seed with a conidial suspension of T . longibrachiatum isolates produced remarkable changes in terms of early seed germination and improved seedling vigour when compared with the untreated control.…”

“…These limitations in the control of Fusarium wilt disease have led researchers to focus on diverse alternative systems. Amongst these alternatives, beneficial microorganisms have gained considerable attention as an eco-friendly and cost-effective platform for the stimulation of disease resistance through induced systemic resistance (ISR), and for the promotion of growth in plants for sustainable crop production (Abdelrahman et al, 2016;Elsharkawy et al, 2012;Harel et al, 2014;Jogaiah et al, 2013Jogaiah et al, , 2016Murali et al, 2013;Nagaraju et al, 2012;Pel and Pieterse, 2013;Pieterse et al, 2014).…”

A Study on the Biological Control of Fusarium oxysporum Using Trichoderma spp., on Soil and Rockwool Substrates in Controlled Environment

“…has shown numerous positive results, but for hydroponics, the application method has not been studied enough, yet. New studies proved that the biological control can stimulate the secondary metabolism resulting in higher concentrations of bioactive substances (Abdelrahman et al, 2016).…”

Fungos promotores do crescimento vegetal e da fitorremediação de metais pesados em combinação com biochar

“…Para alguns fungos promotores de crescimento vegetal, há certa documentação científica da sua biologia e efeitos em insetos, pragas e plantas, como por exemplo: Beauveria bassiana, Metarhizium anisopliae, Pochonia chlamydosporia, Purpureocillium lilacinum e Trichoderma asperella (LOPEZ; SWORD, 2015;ABDELRAHMAN et al, 2016;ESCUDERO et al, 2016;MARRI et al, 2016 .…”