Disseminated<i>Scopulariopsis brevicaulis</i>infection in an allogeneic stem cell recipient: case report and review of the literature

Salmon, Anne-Marie; Debourgogne, Anne; Vasbien, M.; Clement, L.; Collomb, J.; Plenat, F; Bordigoni, Pierre; Machouart, M.

doi:10.1111/j.1198-743x.2009.02878.x

Cited by 5 publications

(6 citation statements)

References 43 publications

(43 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…are high, and those of amphotericin B, voriconazole, and ketoconazole are variable [ 8 – 13 ]. Case reports also describe high-level resistance of S. brevicaulis and S. acremonium to amphotericin B, which correlated with poor clinical outcomes [ 14 – 16 ].…”

Section: Discussionmentioning

confidence: 99%

InvasiveMicroascus trigonosporusSpecies Complex Pulmonary Infection in a Lung Transplant Recipient

Schoeppler

Zamora

Northcutt

et al. 2015

Case Reports in Transplantation

View full text Add to dashboard Cite

Because of the high incidence of morbidity and mortality associated with invasive fungal infections, antifungal prophylaxis is often used in solid organ transplant recipients. However, this prophylaxis is not universally effective and may contribute to the selection of emerging, resistant pathogens. Here we present a rare case of invasive infection caused by Microascus trigonosporus species complex in a human, which developed during voriconazole prophylaxis in a lung transplant recipient. Nebulized liposomal amphotericin B was used in addition to systemic therapy in order to optimize antifungal drug exposure; this regimen appeared to reduce the patient's fungal burden. Despite this apparent improvement, the patient's pulmonary status progressively declined in the setting of multiple comorbidities, ultimately leading to respiratory failure and death.

show abstract

Section: Discussionmentioning

confidence: 99%

InvasiveMicroascus trigonosporusSpecies Complex Pulmonary Infection in a Lung Transplant Recipient

Schoeppler

Zamora

Northcutt

et al. 2015

Case Reports in Transplantation

View full text Add to dashboard Cite

show abstract

“…Few cases of endocarditis due to Scopulariopsis brevicaulis have been reported in the literature [ 2 , 7 – 10 ]. Scopulariopsis species are filamentous fungi that are ubiquitous in the environment and are a known cause of onchomycosis; however, they rarely cause deep infections, such as endocarditis.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, the diagnosis is often based on a combination of histopathologic features and microbiologic data including culture and molecular testing but can be difficult initially, as many fungi that cause endocarditis may look very similar under the microscope. For instance, Scopulariopsis species may demonstrate hyaline or septate hyphae which could potentially appear very similar to Aspergillus , Mucor , Scedosporium , or Candida species; therefore, identification of the etiological agent is necessary for providing appropriate antifungal management of such patients [ 4 , 8 , 10 ].…”

Section: Discussionmentioning

confidence: 99%

“…Given the rarity of such infections, optimal therapy remains undefined [ 11 ]. Combination therapy with amphotericin B, an echinocandin, and/or voriconazole or posaconazole has been utilized and may be reasonable despite the high levels of in vitro resistance as synergy has been reported for some isolates [ 4 , 9 , 10 ]. Despite lack of susceptibility demonstrated in vitro, some success has been seen clinically [ 4 , 8 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A Case ofScopulariopsis brevicaulisEndocarditis with Mycotic Aneurysm in an Immunocompetent Host

Cawcutt

Baddour

Burgess

2015

Case Reports in Medicine

View full text Add to dashboard Cite

Scopulariopsis is a genus of mold that is usually associated with onychomycosis and rarely causes complicated infection in immunocompetent persons. We describe a case of an immunocompetent 65-year-old male with a history of mitral valve repair with prosthetic ring placement who developed acute left posterior knee pain. Imaging showed a left popliteal artery aneurysm and thrombus, and further evaluation with transesophageal echocardiogram demonstrated two large, mobile mitral valve vegetations. He underwent debridement and replacement of the mitral valve, followed by debridement of the left popliteal artery with peroneal artery bypass. The intraoperative cultures grew Scopulariopsis brevicaulis. Due to the resistant nature of the organism, he was initially treated with combination antifungals including liposomal amphotericin B, caspofungin, and voriconazole and was continued on chronic suppression with posaconazole with no evidence of recurrence. Scopulariopsis is a rare cause of fungal endocarditis. Treatment of Scopulariopsis endocarditis is challenging and is not well understood due to its rarity.

show abstract

“…Yet, it is still difficult to perform species identification based on morphological criteria. Moreover, Microascus/Scopulariopsis infections, and disseminated infections in particular might be clinically and histologically indistinguishable from aspergillosis, fusariosis or zygomycosis [ 43 , 44 ]. Since, in the majority of clinical reports on Scopulariopsis spp.…”

Section: Introductionmentioning

confidence: 99%

Rapid Assays for Specific Detection of Fungi of Scopulariopsis and Microascus Genera and Scopulariopsis brevicaulis Species

2016

View full text Add to dashboard Cite

PurposeFungi of Scopulariopsis and Microascus genera cause a wide range of infections, with S. brevicaulis being the most prevalent aetiological agent of mould onychomycosis. Proper identification of these pathogens requires sporulating culture, which considerably delays the diagnosis. So far, sequencing of rDNA regions of clinical isolates has produced ambiguous results due to the lack of reference sequences in publicly available databases. Thus, there is a clear need for the development of new molecular methods that would provide simple, rapid and highly specific identification of Scopulariopsis and Microascus species. The objective of this study was to develop simple and fast assays based on PCR and real-time PCR for specific detection of fungi from Scopulariopsis and Microascus genera, and separately, S. brevicaulis species.MethodsOn the basis of alignment of β-tubulin gene sequences, Microascus/Scopulariopsis-specific primers were designed and S. brevicaulis-specific primers were reevaluated. DNA from cultured fungal isolates, extracted in a two-step procedure, was used in Microascus/Scopulariopsis-specific and S. brevicaulis-specific PCR and real-time PCR followed by electrophoresis or melting temperature analysis, respectively.ResultsThe specificity of the assays was confirmed, as positive results were obtained only for Scopulariopsis spp. and Microascus spp. isolates tested in Microascus/Scopulariopsis-specific assay, and only for S. brevicaulis and S. koningii (syn. S. brevicaulis) isolates in a S. brevicaulis-specific assay, respectively, and no positive results were obtained neither for other moulds, dermatophytes, yeast-like fungi, nor for human DNA.ConclusionsThe developed assays enable fast and unambiguous identification of Microascus spp. and Scopulariopsis spp. pathogens.

show abstract

DisseminatedScopulariopsis brevicaulisinfection in an allogeneic stem cell recipient: case report and review of the literature

Cited by 5 publications

References 43 publications

InvasiveMicroascus trigonosporusSpecies Complex Pulmonary Infection in a Lung Transplant Recipient

InvasiveMicroascus trigonosporusSpecies Complex Pulmonary Infection in a Lung Transplant Recipient

A Case ofScopulariopsis brevicaulisEndocarditis with Mycotic Aneurysm in an Immunocompetent Host

Rapid Assays for Specific Detection of Fungi of Scopulariopsis and Microascus Genera and Scopulariopsis brevicaulis Species

Contact Info

Product

Resources

About